| SUNDAY |
| 2:00 pm - 9:00 pm | Arrival and Check-in |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | New collagens |
| The analysis of the human genome suggest that there are more than 40 bona fide collagen genes and perhaps an equal number of genes that harbor short or extended sequences that bear a triple helical signature. The collagen genes encode the chains of at least 27 collagen types with a marked diversity of structures and tissue-specific functions. Collagenous matrix proteins are found in almost every part of the vertebrate organism and in many non-vertebrates in a rich structural variety that includes fibril- and microfibril-forming collagens, network-forming collagens, fibril-associated collagens and transmembrane proteins; and soluble proteins with collagenous domains such as complement factors. Collagens are not only responsible for tissue integrity and stability, but also regulate cellular differentiation and morphogenetic processes during development and tissue remodeling. In addition, alterations in collagen metabolism lead to a large spectrum of diseases, including common disorders like osteoarthritis, atherosclerosis and fibrotic diseases. The genomic analysis will further our understanding of the structure, evolution, and functions of collagens and their interplay with other matrix macromolecules and should help to direct new studies of collagen functions. |
| 7:30 pm - 7:45 pm | Discussion Leader: Florence Ruggiero
Introduction |
| 7:45 pm - 8:15 pm | Ulrike Hopfer
Targeted disruption of Col8a1 and Col8a2 genes in mice leads to anterior segment abnormalities in the eye |
| 8:15 pm - 8:45 pm | Manuel Koch
New collagens: From type XXII onwards |
| 8:45 pm - 9:00 pm | Rebecca Hjorten
Collagen XXVII: A possible mineralization connection in hard tissues |
| 9:00 pm - 9:15 pm | Francois X. Maquart
The NC1 domain of type XIX collagen inhibits tumor growth |
| 9:15 pm - 9:30 pm | Barbara Maertens
Gliomedin: Characterization of a novel transmembrane collagen located at nodes of Ranvier |
| MONDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | Expression, biosynthesis and regulation of collagens |
| The collagen "transcriptome", the complex of genes that is coordinately regulated in a cell or tissue, is now being defined by use of expression arrays, definition of genes required in exogenous settings such as yeast for the production of intact molecules, the analysis of the effects of mutations in collagen genes or their modifying enzymes and the analysis of knock-out mice. In addition, many collagen types come in alternatively spliced isoforms, and the regulation of splicing mechanisms will also be addressed. Collagen biosynthesis requires an unusually large number of co-translational and post-translational modifications, many of which are unique to collagens and proteins with collagen-like sequences. There are 3-4 isoenzymes of human prolyl 4-hydroxylase, at least 4 lysyl hydroxylase genes, several forms of procollagen -proteinases, and at least 4-5 isoenzymes of lysyl oxidase. Homozygous knock-out mice for many of these loci have been generated and naturally occurring human genetic diseases that result in null alleles have been discovered. |
| 9:00 am - 9:15 am | Discussion Leader: Johanna Myllyharju
Introduction |
| 9:15 am - 9:50 am | Linda Sandell
Transcriptional and post-transcriptional regulation of cartilage genes |
| 9:50 am - 10:10 am | Volkhard Lindner
Collagen triple helix repeat containing-1, a novel regulator of collagen matrix synthesis |
| 10:10 am - 10:50 am | Coffee Break / Photo |
| 10:50 am - 11:25 am | Janice Vranka
Post-translational modifications: Prolyl 3-hydroxylases |
| 11:25 am - 12:00 pm | Raija Soininen
Functions of collagen modifying enzymes analyzed in knock-out mouse models |
| 12:00 pm - 12:15 pm | Vera Hintze
Tight binding or fast cleaving: The interaction of recombinant subdomains of the procollagen C-proteinase with procollagen I |
| 12:15 pm - 12:30 pm | Amy Bradshaw
SPARC Influences post-translational events in the maturation of collagen I |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | Protease processing of matrix |
| The traditional focus of proteolysis of collagens has been the degradation of the extracellular matrix. However, it becomes increasingly clear that proteolytic processing is pivotal to the functions of collagens. Almost universally, collagens are subject to proteolytic activation in order to attain their aggregation competence. In addition, collagen processing also is important for the direction of cellular functions, including cell proliferation, migration and metabolism. Further elucidation of the roles of processing at the cell surface and within the matrix on the generation of the structures, regulation and functions of the various matrix assemblies is a growing area of interest. In addition, collagen precursor chains are degraded during or immediately after synthesis by cytosolic proteosomes or endogenous ER proteases. The determinants of degradation, the pathways by which nascent chains reach degradation sites, and the mechanisms by which these pathways are controlled will be of interest. |
| 7:30 pm - 7:45 pm | Discussion Leader: Suneel Apte
Introduction |
| 7:45 pm - 8:15 pm | Carl Blobel
Role of ADAMs in EGF-receptor signaling and cancer |
| 8:15 pm - 8:30 pm | Hideaki Nagase
Mechanism of MMP-1 cleavage of collagens: Helix stability and enzyme activity |
| 9:00 pm - 9:15 pm | Dan Greenspan
Extracellular modulation of BMP-1/Tolloid-like proteinase function |
| 9:15 pm - 9:30 pm | Walter Stoecker
Procollagen c-proteinase unlimited |
| TUESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | Supramolecular matrix assemblies |
| The mechanism of collagen organization at the level of fibrils, microfibrils, networks, and other supramolecular assemblies in the extracellular matrix are only partly understood yet crucial to the analysis of tissue function in health and disease. And the cellular and molecular tools controlling organization of such suprastructures into tissue architectures is even less well understood. In this context, collagens cooperate with non-collagenous matrix macromolecules. Recent work has focused on defining the peptide sequence motifs in fibrillar and nonfibrillar collagens that direct the specificity of supramolecular assembly using site-directed mutagenesis, in vitro assays, and the analysis of tissues or cell and tissue cultures. Recent studies also assigned decisive organizational roles to enzymes processing precursors to aggregation-competent matrix macromolecules, to alloyed mixtures of collagens and non-colllagenous molecules, or to cellular structures guiding tissue assembly (e.g., "fibripositors"). The molecular details of collagen recognition by cell surface receptors will be a central issue. |
| 9:00 am - 9:15 am | Discussion Leader: Paul Bornstein
Introduction |
| 9:15 am - 9:50 am | Karl Kadler
Procollagen trafficking, processing and fibrillogenesis |
| 9:50 am - 10:10 am | Elizabeth Canty
Cellular control of collagen fibril assembly in the late secretory pathway |
| 10:10 am - 10:35 am | Billy Hudson
Molecular mechanism for chain-specific assembly of collagen IV networks |
| 10:35 am - 11:00 am | Coffee Break |
| 11:00 am - 11:35 am | Helene Sage
Assembly by SPARC; a tale of two matrices |
| 11:35 am - 11:55 am | Uwe Hansen
Collagen fibrils as tissue-specific, macromolecular alloys |
| 11:55 am - 12:30 pm | Bob Mecham
Live imaging of ECM assembly: watching it happen |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | Collagens in cell adhesion and migration |
| Collagens are crucial substrates for cells and the collagen receptors on the cell surface are critically involved in cellular responses to clues from the extracellular matrix. The structural basis of integrin interactions with their collagen-like substrates will be of interest. In addition, the number of cell surface proteins interacting with collagens also includes discoidin domain receptors as well as glycoprotein VI which serves as a trigger for platelet aggregation in hemostasis. Animal models of tissue-specifically inactivated matrix receptors shed more light into the complex functions of these molecules. |
| 7:30 pm - 7:45 pm | Discussion Leader: Johannes A. Eble
Introduction |
| 7:45 pm - 8:15 pm | Wolfgang Vogel
Structure-to-function relationships in discoidin-domain collagen receptors |
| 8:15 pm - 8:30 pm | D. Gullberg
Latest insights into the function of α11ß1 integrin |
| 8:30 pm - 8:45 pm | J.K. Kim
Novel binding site on Collagen III for collagen binding integrins |
| 8:45 pm - 9:15 pm | Peter Friedl
Diversity of cell invasion mechanisms through 3D collagen: Integrins and matrix proteases |
| 9:15 pm - 9:30 pm | L. Bruckner-Tuderman
Transmembranous collagens as cell adhesion molecules |
| WEDNESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | Invertebrate and vertebrate animal models |
| Several animal models for matrix functions have been established. Transgenic mice have tought is many important lessons and continue to be powerful tools to answer question concerning the function of collagens in their biological context. In addition, zebra fish have become plentiful providers of information on tissue development and homeostasis and an ever increasing number of invertebrate models becomes available. This no longer is restricted to the "classics" Drosophila melanogaster and Caenorhabditis elegans, but now also includes hydra or planarians, creatures with a tremendous potential of tissue regeneration. |
| 9:00 am - 9:15 am | Discussion Leader: Kathy Cheah, Hong Kong
Introduction |
| 9:15 am - 9:50 am | Tom van Agtmael
Collagen IV mutations define a spectrum of phenotypes affecting eye and kidney |
| 9:50 am - 10:10 am | Doug Gould
Influence of genetic context on pleiotropic phenotypes in mice with a Col4a1 mutation |
| 10:10 am - 10:40 am | Coffee Break |
| 10:40 am - 11:15 am | Kiyokazu Agata
Planarians: A model for gene regulation in development and regeneration |
| 11:15 am - 11:50 am | Ray Boot-Hanford
Characterisation of a knock-in mouse model of metaphyseal chondrodysplasia type |
| 11:50 am - 12:10 pm | Danny Chan
Chondrocytes survive ER stress caused by unfolded collagen X via re-programming |
| 12:10 pm - 12:30 pm | Short Talk TBA |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | Collagen diseases |
| "Experiments of nature" also have contributed to our understanding of matrix function and their investigation will be of importance not only to rare diseases but also to the most common afflictions. In addition, the knowledge gained from studying rare diseases will help us to improve our possibilities to understand and to treat common disorders. Even after more than two decades of research in collagen I mutations, we are still discovering new disease connections of this protein. |
| 7:30 pm - 7:35 pm | Discussion Leader: Leena Ala-Kokko
Introduction |
| 7:35 pm - 8:10 pm | Robert C. Gensure
Novel COL1A1 mutation in infantile cortical hyperostosis (Caffey disease) expands the spectrum of collagen-related disorders |
| 8:10 pm - 8:30 pm | James Pace
Defects of the amino and carboxyl propeptides of type I procollagen |
| 8:30 pm - 8:50 pm | Richard Wenstrup
Ehlers Danlos syndrome: The collagen V - fibrillogenesis connection |
| 8:50 pm - 9:15 pm | Jean Baum
Structural and dynamic consequences of disease-causing collagen mutations |
| 9:15 pm - 9:30 pm | Kathy Cheah
Procollagen IIA regulates BMP signaling in heart development via a regulatory loop |
| THURSDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | Gene therapy and regenerative medicine |
| Stem cells have become the overpowering subject of research into possibilities to regenerate tissues or to genetically modulate tissue functions. Again, the extracellular matrix, and collagens in particular, not only serve as inert scaffolds but also direct stem cells to make use of their phenotypic plasticity and to meet their developmental fates. It also has become apparent that gene targeting also is strongly influence by the static exrtracellular environment. Not only will we improve our means to manipulate cells into desired directions but also we will learn about basic biology from the crucial decisions stem cells take when undergoing matrix interactions. |
| 9:00 am - 9:10 am | Discussion Leader: Leena Bruckner-Tuderman
Introduction |
| 9:10 am - 9:50 am | Darwin J. Prockop
Characterization and potential uses of adult stem cells from bone marrow to repair tissues |
| 9:50 am - 10:30 am | Yann Barrandon
In and out of the stem cell niche |
| 10:30 am - 11:00 am | Coffee Break |
| 11:00 am - 11:40 am | Guerrino Meneguzzi
Gene therapy involving proteins of the dermoepidermal junctions: Collagen VII |
| 11:40 am - 12:00 pm | Angelique Balguid
The collagen organization in heart valves as a target for tissue engineered valves |
| 12:00 pm - 12:20 pm | Short Talk TBA |
| 12:20 pm - 12:30 pm | Summary (Leena Bruckner-Tuderman) |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | Business Meeting / Hot topics |
| Business Meeting |
| Discussion Leader: F. Ramirez
Introduction |
| (Topics and talks will be selected from the abstracts during the conference) |
| FRIDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am | Depart |
