| SUNDAY |
| 2:00 pm - 9:00 pm | Arrival and check-in |
| 6:00 pm | Dinner |
| Session 1. | New Technologies for Studies of Second Messenger Signaling |
| Discussion Leaders: E. Degerman (Univ. of Lund) & S. Francis (Vanderbilt University) |
| 7:30-8:00 | Opening Remarks |
| 8:00-8:30 | Natalie Ahn University of Colorado at Boulder, Boulder, CO
Analysis of regulated protein dynamics by deuterium exchange mass spectrometry |
| 8:30-9:00 | Jeffrey Karpen University of Colorado School of Medicine, Denver, CO
Resolution of cAMP signals in three-dimensional microdomains using novel, real-time sensors. |
| 9:00-9:30 | Wolfgang Dostmann University of Vermont, Burlington, VT
Cygnets: the dynamics of intracellular cGMP revealed by genetically encoded fluorescent indicators |
| 9:45-10:45 pm | Welcome Reception |
| MONDAY |
| 7:30-8:30 | Breakfast |
| 8:45 | Conference Photo |
| Session 2. | Cyclic Nucleotides & Regulation of Energy Metabolism |
| Discussion Leaders: J. Corbin (Vanderbilt University) & M. Houslay (Univ. of Glasgow) |
| 9:00-9:30 | Sven Enerbäck Goteborg University, Goteborg, SWEDEN
FOXC2 is a winged helix gene that counteracts obesity, hypertriglyceridemia and diet induced insulin resistance |
| 9:30-10:00 | Vince Manganiello National Heart and Lung Institute, Bethesda, MD.
Characterization of PDE3B KO mice. |
| 10:00-10:30 | Hideichi Makino Ehime University School of Medicine, Ehime, Japan
Identification of a PDE3B interacting protein using the yeast two-hybrid system |
| 10:30-11:00 | Break |
| 11:00-11:30 | Tamar Michaeli Albert Einstein School of Medicine, New York, NY
PDEs and regulation of insulin secretion and action |
| 11:30-12:00 | Allen Zhao University of Pittsburgh School of Medicine, Pittsburgh, PA
Leptin, PDE3B, and regulation of body weight. |
| 12:00-12:15 | Eva Degerman (Short Talk) University of Lund, Lund, SWEDEN
Role of PDE3B in the regulation of energy metabolism. |
| 12:15-12:30 | Hanguan Liu (Short talk) National Heart and Lung Institute, Bethesda, MD
Characterization of the PDE3B promotor. |
| 12:30 pm | Lunch |
| 1:30 pm - 4:30 pm | Free Time |
| 4:30 pm - 6:00 pm | Poster Session I |
| 6:00 pm | Dinner |
| Session 3. | GAFs and Cyclic nucleotide-binding domains |
| Discussion Leaders: N. Artemyev (Univ. of Iowa) & R. Cote (Univ. of New Hampshire) |
| 7:30-8:00 | L. Aravind National Center for Biotechnology Information, NIH, Bethesda, MD
Evolutionary history and diversity of cNMP cyclases, phosphodiesterases and associated small molecule binding domains. |
| 8:00-8:30 | Joe Beavo University of Washington, Seatttle, WA
PDE2 GAF/cGMP-binding site structure: How good a model for other GAF domains? |
| 8:30-9:00 | Joachim Schultz University of Tuebingen. Tubingen, Germany
A GAF-domain operated cAMP switch: adenylyl cyclase from Anabaena |
| 9:00-9:30 | Rick Cote University of New Hampshire, Durham NH
Allosteric interactions between catalytic and inhibitory subunits of PDE6 mediated by its GAF domains. |
| 9:30-9:45 | Gary Kruh (Short talk) Fox Chase Cancer Center, Philadelphia, PA
Cyclic nucleotide efflux pumps |
| TUESDAY |
| 7:30-8:30 | Breakfast |
| Session 4. | Cyclic Nucleotides & PDEs: Proliferation, Apoptosis, & Differentiation |
| Discussion Leaders: M. Conti (Stanford University) & C. Lugnier (University of Strasbourg) |
| 9:00-9:30 | Leonard Forte University of Missouri School of Medicine, Columbia MO
Gene expression in colon tumors: guanylin and uroguanylin, receptor-guanylate cyclase and cyclic GMP signaling molecules. |
| 9:30-10:00 | Joe Thompson Cell Pathways, Inc., Horsham, PA
SAANDS induce apoptosis via cGMP-mediated mechanisms. |
| 10:00-10:30 | Tom Lincoln University of Alabama at Birmingham, Birmingham, AL
Expression of PKG-I in smooth muscle cells: implications in vascular disease. |
| 10:30-11:00 | Break |
| 11:00-11:30 | Claire Lugnier University of Strasbourg, Illkirch, FRANCE
PDEs as potential targets in angiogenesis. |
| 11:30-11:45 | Dietrich Dettmer (Short talk) University of Leipzig, Leipzig, GERMANY
Behavior of PDE2 and PDE3 during transformation of rat hepatic stellate cells to myofibroblast-like cells |
| 11:45-12:00 | Douglas Tilley (short talk) Queen's University, Kingston, ON, Canada
"Vascular smooth muscle PDE3 and PDE4 activities and levels are regulated by cAMP levels." |
| 12:00-12:15 | Hermann Tenor (short talk) Byk Gulden, Konstanz, Germany
" PDE isoenzyme families in osteoarthritis chondrocytes-functional importance of PDE4." |
| 12:15-12:30 | Adam Lerner (short talk) Boston Medical Center, Boston, MA
Caspase 9 plays a key role in rolipram-induced apoptosis in primary chronic lymphocytic leukemia cells |
| 12:30 pm | Lunch |
| 1:30 pm - 4:30 pm | Free Time |
| 4:30 pm - 6:00 pm | Poster Session I (continued) |
| 6:00 pm | Dinner |
| Session 5. | Selective Expression of PDEs: Regulation & Function |
| Discussion Leaders: C. Jin (Stanford University) & D. Dettmer (Univ. of Leipzig) |
| 7:30-8:00 | Marco Conti , Stanford University Medical Center, Stanford, CA
Structure and function of different PDE4 isoforms. |
| 8:00-8:30 | Don Maurice, Queens University at Kingston, Kingston, Ontario, CANADA
The impact of vascular smooth muscle cell phenotype on phosphodiesterase expression: Impact on regulated cell function. |
| 8:30-9:00 | Carolyn Smith , DesignWrite, Princeton, NJ
Regulation of cAMP PDE3 and cGMP PDE5 genes in cardiovascular injury (heart failure and hypertension). |
| 9:00-9:30 | Ching Lin , University of California, San Francisco
Human PDE5A gene expression and regulation. |
| 9:30-9:45 | Chen Yan (Short talk) University of Rochester, Rochester, NY
Regulation of PDE1A gene expression in vascular smooth muscle cells. |
| WEDNESDAY |
| 7:30-8:30 | Breakfast |
| Session 6. | Compartmentalization of Cyclic Nucleotides & Their Intracellular Targets |
| Discussion Leaders: D. Maurice (Queen's University) & C. Smith (DesignWrite) |
| 9:00-9:30 | Laurence Brunton University of California-San Diego, La Jolla CA
Compartmentation of cyclic nucleotide signaling in cardiac cells |
| 9:30-10:00 | Rodolphe Fischmeister Universite de Paris-Sud, Paris. France
Contribution of different PDE subtypes to cAMP compartmentation in cardiac myocytes |
| 10:00-10:15 | Manuela Zaccolo (Short Talk) University of Padua, Padua, ITALY
cAMP microdomains in heart cells. |
| 10:15-10:45 | Break |
| 10:45-11:15 | Miles Houslay University of Glasgow, Glasgow, SCOTLAND
Intracellular targeting of PDE4 isoforms |
| 11:15-11:45 | Kjetil Tasken University of Oslo, Oslo, NORWAY
PKA-PDE signalling units |
| 11:45-12:00 | Jun Kotera (short talk) Tanabe Seiyaku Company, Toda, Saitama, JAPAN
Subcellular localization of phosphodiesterase 10A variants |
| 12:00-12:15 | Matthew Movsesian (short talk) University of Utah, Salt Lake City, UT
Intracellular compartmentation of PDE3 activity and effects of cAMP and
cGMP hydrolysis in cardiac and vascular myocytes |
| 12:15-12:30 | Wilma van Staveren Maastricht University, Maastricht,
The Netherlands
Characterization of the cellular localization of cGMP-hydrolyzing PDEs in the rat hippocampus and related structures |
| 12:30 pm | Lunch |
| 1:30 pm - 4:30 pm | Free Time |
| 4:30 pm - 6:00 pm | Poster Session II |
| 6:00 pm | Dinner |
| Session 7. | Pharmacological Targeting of Cyclic Nucleotide Signaling Pathways |
| Discussion Leaders: M. Barnette (GlaxoSmithKline) & R. Colman (Temple Univ.) |
| 7:30-8:00 | Natalie Mount Pfizer Global Research and Development, Sandwich, ENGLAND
New therapeutic opportunities for PDE5 inhibitors. |
| 8:00-8:30 | Jun-ichi Kambayashi Otsuka Maryland Research Institute, Rockville, MD
Current progress in clinical and pre-clinical research on cilostazol |
| 8:30-9:00 | Erwin Bischoff Bayer-AG
Physiological effects of pharmacologic cGMP elevation. |
| 9:00-9:30 | Zheng Huang Merck Frosst, Pointe-Claire-Dorval, CANADA
Mechanistic insights into PDE4 catalyzed cAMP hydrolysis and implications to inhibitor design. |
| 9:30-9:45 | Peter Schafer (short talk) Celgene Corporation, Warren, NJ
CC-110004: a novel PDE4 inhibitor with an improved therapeutic index in a lung neutrophilia model |
| THURSDAY |
| 7:30-8:30 | Breakfast |
| Session 8. | Insights into Structure & Function of PDEs |
| Discussion Leaders: J. Beavo (University of Washington) & J. Thompson (Cell Pathways, Inc.) |
| 9:00-9:30 | Hengming Ke University of North Carolina at Chapel Hill, Chapel Hill, NC
Crystal structure of the PDE4-rolipramcomplex provides insight into the binding and the inhibitor selectivity. |
| 9:30-10:00 | Robert Colman Temple University School of Medicine, Philadelphia, PA
PDE3A: Active and inhibitory sites as revealed by mutagenesis, affinity labeling, and molecular modeling. |
| 10:00-10:30 | Nikolai Artemyev University of Iowa School of Medicine, Iowa City IA
Structural determinants of PDE6 function |
| 10:30-11:00 | Break |
| 11:00-11:30 | Ted Wensel Baylor College of Medicine, Houston, TX
Regulation of PDE6 in rod and cone photoreceptors. |
| 11:30-12:00 | Jackie Corbin/Sharron Francis Vanderbilt University School of Medicine, Nashville, TN
Allosteric and catalytic sites of PDE5 |
| 12:00-12:15 | Kenji Omori (Short talk) Tanabe Seiyaku Company, Toda, Saitama, JAPAN
Comparison of characteristics of PDE8 family |
| 12:15-12:30 | Wito Richter (short talk) Stanford University, Stanford, CA
Involvement of upstream conserved regions in the dimerization of the
cAMP-specific PDE4 |
| 12:30 pm | Lunch |
| 1:30 pm - 4:30 pm | Free Time |
| 4:30 pm - 6:00 pm | Poster Session II (continued) |
| 6:00 pm | Dinner |
| Session 9. | Selected PDEs: Specificity in Catalysis, Regulation & Function |
| Discussion Leaders: V. Manganiello (National Institutes of Health) & T. Michaeli (Albert Einstein) |
| 7:30-8:00 | Business Session |
| 8:00-8:30 | Akio Yamazaki Wayne State University School of Medicine, Detroit, MI
Turnoff of GTP-activated PDE6 withouth GTP hydrolysis: role of cyclin-dependent protein kinase 5. |
| 8:30-9:00 | Doris Koesling Ruhr-Universitat Bochum, Bochum, GERMANY
A new regulatory mechanism within NO/cGMP signalling |
| 9:00-9:15 | Karnam Murthy (Short Talk) Medical College of Virginia, Richmond, VA
Regulation of PDE5 and soluble guanylate cyclase by cGMP-dependent
protein kinase in smooth muscle |
| 9:15-9:30 | Kelley Bentley Univ. of Michigan, Ann Arbor, MI
Increases in PDE2 phosphorylation by an associated NGF-stimulated kinase |
| FRIDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am | Departure |
