Sunday, May 14
7:30 p.m. - 9:30 p.m. | Biotechnological approaches in glyco/sphingolipid research |
Chairperson/Discussion Leader: Y. Nagai (Tokyo, Japan), S. Sonnino (Milan, Italy)
| 7:20 - 7:30 |
Opening comments from Site Staff and Conference Chair |
| 7:30 - 7:45 |
Introductory Lecture Y. Nagai (Tokyo, Japan): "On the possibility of developing super glycobiology" |
| 7:45 - 8:00 |
R.L. Proia (Bethesda, MD, U.S.A.): "Functions of sphingolipids revealed by gene targeting" |
| 8:00 - 8:08 |
Discussion |
| 8:08 - 8:23 |
G. Schwarzmann (Bonn, Germany): "Glycolipid distribution and traffic in cells: studies with labeled analogs" |
| 8:23 - 8:31 |
Discussion |
| 8:31 - 8:46 |
A. Pavan (L'Aquila, Italy): "Recognition of cell endogenous sphingolipids by a new electromicroscopic procedure" |
| 8:46 - 8:54 |
Discussion |
| 8:54 - 9:09 |
T. Taki (Tukushima, Japan): "Preparation of glyco-replica peptides and their medical application" |
| 9:09 - 9:17 |
Discussion |
| 9:17 - 9:27 |
C. Sullards (Atlanta, GA, U.S.A.): "Identification and quantitation of sphingolipids by tandem mass spectrometry" |
| 9:27 - |
Discussion |
Monday, May 15
9:00 a.m. - 12:30 p.m. | Medical implications of glyco/sphingo-lipids. I |
Chairperson/ Discussion Leader: H. Willison (Glasgow, U.K.), R.L. Schnaar (Baltimore, MD, U.S.A.)
| 9:00 - 9:20 |
Introductory Lecture: H. Willison (Glasgow, U.K.): "The origin and characteristics of anti-glycolipid antibodies in human neuropathies" |
| 9:20 - 9:35 |
A. Moran (Galway, Ireland): "Campylobacter jejuni and Guillain-Barré syndrome" |
| 9:35 - 9:43 |
Discussion |
| 9:43 - 9:53 |
G. De Libero (Basel, Switzerland): "Glyco/sphingolipids as new auto-antigens stimulating T cells in autoimmune diseases" |
| 9:53 - 10:00 |
Discussion |
| 10:00 - 10:50 |
Coffee Break and Conference Photo |
| 10:50 - 11:05 |
G. Ritter (New York, U.S.A.): "Towards fully synthetic ganglioside cancer vaccines: Synthetic GM2 as a novel immunogen in melanoma vaccines" |
| 11:05 - 11:13 |
Discussion |
| 11:13 - 11:28 |
E. Noll (Boston, U.S.A.): "GM3: a novel and selective inducer of apoptosis in malignant human gliomas" |
| 11:28 - 11:36 |
Discussion |
| 11:36 - 11:51 |
M. Cabot (Santa Monica, CA, U.S.A.): "Control of chemotherapy resistance through regulation of ceramide glycosylation" |
| 11:51 - 11:59 |
Discussion |
| 11:59 - 12:14 |
W.H. Holleran (San Francisco, CA, U.S.A.): "Omega-hydroxy ceramides: formation and function" |
| 12:14 - |
Discussion |
| 4:00 - 5:30 |
Poster Session |
Monday, May 15
5:30 p.m. - 7:30 p.m. | Medical implications of glyco/sphingo-lipids. II |
Chairperson /Discussion Leader: M. Saito (Tokyo, Japan), G. Van Meer (Amsterdam, The Netherlands)
| 5:30 - 5:35 |
Chair Introduction |
| 5:35 - 5:50 |
K. Suzuki (Chapel Hill, NC, U.S.A.): "Manipulation of sphingolipidosis phenotype by cross-breeding with other mutants" |
| 5:50 - 5:58 |
Discussion |
| 5:58 - 6:13 |
F.M. Platt (Oxford, U.K.): "Substrate deprivation therapy for the glycosphingolipidoses" |
| 6:13 - 6:21 |
Discussion |
| 6:21 - 6:36 |
J. Shayman (Ann Arbor, Mich, U.S.A.): "Treatment of α-galactosidase: A deficiency with third generation glucosylceramide synthase inhibitors" |
| 6:36 - 6:44 |
Discussion |
| 6:44 - 6:54 |
T. Miyagi (Miyagi, Japan): "Development of non-insulin-dependent diabetes mellitus in the transgenic mice over-expressing a ganglioside sialidase" |
| 6:54 - 6:59 |
Discussion |
| 6:59 - 7:09 |
W. Drobnik (Regensburg, Germany): "Concentration of distinct plasma ceramides is increased in patients with advanced atherosclerosis: role for ceramides as proinflammatory systemic mediators?" |
| 7:09 - 7:14 |
Discussion |
| 7:14 - 7:24 |
R. Kannagi (Aichi, Japan): "Carbohydrate ligands for selectins on leukocytes and endothelial cells: roles of sulfation and sialylation" |
| 7:24 - |
Discussion |
Tuesday, May 16
9:00 a.m. - 12:30 a.m. | Membrane domains and glyco/sphingo-lipids: structural and functional aspects. I |
Chairpersons/Discussion Leaders: S.I. Hakomori (Seattle, WA, USA), A. Pavan (L'Aquila, Italy)
| 9:00 - 9:05 |
Chair Introduction |
| 9:05 - 9:20 |
D. Toomre (Heidelberg, Germany): "Size and dynamics of lipid rafts" |
| 9:20 - 9:28 |
Discussion |
| 9:28 - 9:43 |
T.V. Kurzchalia (Berlin, Germany): "Membrane microdomains and glycolipids" |
| 9:43 - 9:51 |
Discussion |
| 9:51 - 10:01 |
M. Masserini (Milan, Italy): "Supramolecular organization of lipids in caveole: new findings" |
| 10:01 - 10:06 |
Discussion |
| 10:06 - 10:26 |
Coffee Break |
| 10:26 - 10:41 |
S. Sonnino (Milan, Italy): "Sphingolipid enriched domains in cultured cerebellar neurons" |
| 10:41 - 10:49 |
Discussion |
| 10:49 - 11:04 |
R.L. Schnaar (Baltimore, MD, U.S.A.): "Structural and functional implications of ganglioside/lectin binding in the brain" |
| 11:04 - 11:12 |
Discussion |
| 11:12 - 11:27 |
S. Pfeiffer (Farmington, CT, U.S.A.): "Structure and function of glycosphingolipid/ cholesterol rafts in myelin" |
| 11:27 - 11:35 |
Discussion |
| 11:35 - 11:50 |
K. Kasahara (Tokyo, Japan): "Involvement of glycosphingolipid in GPI-anchored neuronal cell adhesion molecule TAG-1 signaling via src-family kinase Lyn in lipid rafts" |
| 11:50 - 11:58 |
Discussion |
| 11:58 - 12:08 |
A. Tepper (Amsterdam, The Netherlands): "Sphingomyelin hydrolysis to ceramide during the execution phase of apoptosis results from loss of plasma membrane phospholipid asymmetry and alters cell surface morphology" |
| 12:08 - 12:13 |
Discussion |
| 12:13 - 12:23 |
E. Gulbins (Tuebingen, Germany): "The role of acid sphingomyelinase and ceramide in receptor clustering" |
| 12:23 - |
Discussion |
| 4:00 - 5:30 |
Poster Session |
Tuesday, May 16
5:30 p.m. - 7:30 p.m. | Membrane domains and glyco/sphingo-lipids: structural and functional aspects. II |
Chairpersons/Discussion Leaders: T.V. Kurzchalia (Berlin, Germany), G. Tettamanti (Milan, Italy)
| 5:30 - 5:35 |
Chair Introduction |
| 5:35 - 5:50 |
S.I. Hakomori (Seattle, WA, USA): "Disruption and reconstitution of glycolipid signalling domain" |
| 5:50 - 5:58 |
Discussion |
| 5:58 - 6:13 |
C. Lingwood (Toronto, Canada): "Use of retrograde transport of globotriaosyl ceramide to target exogenous DNA to the nucleus" |
| 6:13 - 6:21 |
Discussion |
| 6:21 - 6:36 |
J.I. Inokuchi (Sapporo, Japan): "Glycosphingolipid deficiency affects functional microdomain formation in Lewis lung carcinom cells" |
| 6:36 - 6:44 |
Discussion |
| 6:44 - 6:54 |
A. Draeger (Bern, Switzerland): "Cell membrane dynamics: Ca2+- regulated association of lipid microdomains" |
| 6:54 - 6:59 |
Discussion |
| 6:59 - 7:09 |
L.K. Vogel (Copenhagen, Denmark) "Studies on apical targeting and raft association, using the serpin family as model proteins" |
| 7:09 - 7:14 |
Discussion |
| 7:14 - 7:24 |
T. Mutoh (Fukui, Japan) "Unglycosylated Trk protein does not co-localize nor associate with ganglioside GM1 in stable clone over-expressing Trk of PC12 cells" |
| 7:24 - |
Discussion |
Wednesday, May 17
9:00 a.m. - 12:30 a.m. | Sphingolipids in signalling and regulatory processes. I |
Chairperson/Discussion Leader: Y.A. Hannun (Charleston, S.C., USA), S. Spiegel (Washington, DC, USA)
| 9:00 - 9:20 |
Introductory Lecture: Y.A. Hannun (Charleston, S.C., USA): "Sphingolipid metabolism in cell regulation" |
| 9:20 - 9:35 |
R.N. Kolesnik (New York, N.Y., USA): "Lessons from the acid sphingomyelinase knockout mouse" |
| 9:35 - 9:43 |
Discussion |
| 9:43 - 9:58 |
T. Futerman (Rehovot, Israel): "Regulatory roles for sphingolipids in neuronal development" |
| 9:58 - 10:06 |
Discussion |
| 10:06 - 10:26 |
Coffee Break |
| 10:26 - 10:41 |
L. Riboni (Milan, Italy): "Regulatory role of ceramide in glial cell proliferation" |
| 10:41 - 10:49 |
Discussion |
| 10:49 - 11:04 |
T. Levade (Toulouse, France): "Role of FAN (Factor Associated with Neutral sphingomyelinase activation) in cytokine-induced apoptosis" |
| 11:04 - 11:12 |
Discussion |
| 11:12 - 11:22 |
M. Ito (Fukuoka, Japan): "Molecular cloning of neutral/alkaline ceramidases involved in sphingolipid signaling" |
| 11:22 - 11:27 |
Discussion |
| 11:27 - 11:37 |
G. Dawson (Chicago, IL, U.S.A.): "Protein palmitoylation and ceramide-induced apoptosis" |
| 11:37 - 11:42 |
Discussion |
| 11:42 - 11:52 |
B.J. Kroesen (Groningen, The Netherlands): "The involvement of sphingolipid metabolism in B-cell receptor induced apoptosis" |
| 11:52 - 11:57 |
Discussion |
| 11:57 - 12:07 |
R. Bansal (Farmington, CT, USA): "Negative regulation of oligodendrocyte differentiation by galactosphingolipids" |
| 12:07 - 12:12 |
Discussion |
| 12:12 - 12:22 |
T. Tai (Tokyo, Japan): "Characterization of galactosylceramide expression factor-1 (HGF-regulated tyrosine kinase substrate) in epithelial cells" |
| 12:22 - |
Discussion |
| 4:00 - 5:30 |
Poster Session |
Wednesday, May 17
5:30 p.m. - 7:30 p.m. | Sphingolipids in signalling and regulatory processes. II |
Chairpersons/Discussion Leaders: R.N. Kolesnick (New York, USA), Y.T. Li (New Orleans, LA, USA)
| 5:30 - 5:35 |
Chair Introduction |
| 5:35 - 5:50 |
S. Spiegel (Washington, DC, USA): "The role of sphingosine-1-phosphate in angiogenesis" |
| 5:50 - 5:58 |
Discussion |
| 5:58 - 6:08 |
P. Xia (Adelaide, Australia): "Sphingosine kinase acts as an oncogene" |
| 6:08 - 6:13 |
Discussion |
| 6:13 - 6:28 |
H. Higashi (Tokyo, Japan): "Saccharide signals to stimulate intracellular protein kinases and cytoskeletal reorganization via a cell surface receptor" |
| 6:28 - 6:36 |
Discussion |
| 6:36 - 6:46 |
D. Perry (Charleston, S.C., USA): "Regulation of de novo ceramide synthesis in mammalian stress response" |
| 6:46 - 6:51 |
Discussion |
| 6:51 - 7:01 |
M. Hedlund (Lund, Sweden): "Glycoshingolipid dependent activation of epithelial cytokine responses" |
| 7:01 - 7:06 |
Discussion |
| 7:06 - 7:21 |
P. Joshi ( Bangalore, India): "Signalling events activated by anti glycosphingolipid antibodies in neural cells" |
| 7:21 - |
Discussion |
Thursday, May 18
9:00 a.m. - 12:30 a.m. | Metabolism and trafficking of glyco/sphingo-lipids |
Chairperson/Discussion Leader: A. Futerman (Rehovot, Israel), A.H. Merrill (Atlanta, GA, USA)
| 9:00 - 9:20 |
Introductory Lecture: K. Sandhoff (Bonn, Germany): "Intra- and extracellular membrane digestion: topology and mechanism" |
| 9:20 - 9:35 |
G. Van Meer (Amsterdam, The Netherlands): "Glucosylceramide on the cytosolic surface: substrate for MDR1 P-glycoprotein and function in protein transport" |
| 9:35 - 9:43 |
Discussion |
| 9:43 - 9:58 |
Y. Hirabayashi (Tokyo, Japan): "Biological functions of ceramide glycosylation in vivo: lessons from Drosophila" |
| 9:58 - 10:06 |
Discussion |
| 10:06 - 10:26 |
Coffee Break |
| 10:26 - 10:41 |
A.H. Merrill (Atlanta, GA, U.S.A.): "Regulation of de novo sphingolipid biosynthesis" |
| 10:41 - 10:49 |
Discussion |
| 10:49 - 11:04 |
S.C. Basu (Notre Dame, IN, U.S.A.): " Characterization of ceramide glycanase (acid and neutral pH-optima) from developing brain tissues" |
| 11:04 - 11:12 |
Discussion |
| 11:12 - 11:22 |
H.J. Maccioni (Cordoba, Argentina) "Functional association between GM2- and GM1-synthase in Golgi membranes" |
| 11:22 - 11:27 |
Discussion |
| 11:27 - 11:42 |
Y.T. Li (New Orleans, LA, U.S.A.): "Catabolism of GM2 ganglioside" |
| 11:42 - 11:50 |
Discussion |
| 11:50 - 12:00 |
C.S. Wright (Charlottesville, VA, USA): "Crystal structure of GM2 activator protein: implications of a novel ß-sheet fold for glycolipid binding" |
| 12:00 - 12:05 |
Discussion |
| 12:05 - 12:20 |
S. Ladisch (Washington, DC, U.S.A.): "Molecular strategies to alter glycosphingolipid metabolism, and effects on tumorigenesis" |
| 12:20 - |
Discussion |
| 3:30 - 5:30 p.m. |
Business Meeting (Chair: A.H. Merrill) |
Thursday, May 18
5:30 p.m. - 7:30 p.m. | Structural biology and genetics of glyco/sphingolipids |
Chairpersons/Discussion Leaders: K. Suzuki (Chapel Hill, NC, USA), K. Sandhoff (Bonn, Germany)
| 5:30 - 5:50 |
Introductory lecture: M. Saito (Tokyo, Japan): "Molecular characterisation of ganglioside GM3 synthetic sialytransferase: its expression characteristics and genomic structure in humans and mice" |
| 5:50 - 6:05 |
A. Suzuki (Tokyo, Japan): "Kidney tubular cell specific regulation of ß1-6G1cNAc transferase transcription" |
| 6:05 - 6:12 |
Discussion |
| 6:12 - 6:27 |
R.C. Dickson (Lexington, KY, USA): "The role of sphingolipid long chain base phosphates in yeast stress response" |
| 6:27 - 6:34 |
Discussion |
| 6:34 - 6:49 |
L. Obeid (Charleston, SC, U.S.A.): "Yeast genetic approaches to study sphingolipid metabolism and cell regulation" |
| 6:49 - 6:56 |
Discussion |
| 6:56 - 7:11 |
K. Honke (Osaka, Japan): "Glycolipid sulfotransferase knockout mouse" |
| 7:11 - 7:18 |
Discussion |
| 7:18 - 7:28 |
K. Furukawa (Nagoya, Japan): "Structure and function of CD77/Gb3 synthase gene" |
| 7:28 - |
Discussion |
|
