| Sunday |
| 2:00-11:00 PM | Arrival and Registration |
| 6:00 PM | Dinner |
| Genetic and physiology of processing I |
| 7:15 | Welcoming remarks |
| 7:30 | Intro by discussion leader: Donald F. Steiner, University of Chicago |
| 7:45 | C.J. Grimmelikhuijzen, University of Copenhagen: Neurohormone Biosynthesis in The Primitive Nervous Systems of Cnidarians |
| 8:15 | Paul Taghert, Washington University: Processing enzymes and their substrates in Drosophila |
| 8:45 | Jan Christian, Oregon Health Sciences University: Regulation of embryonic cell-cell signaling by proprotein convertases. |
| 9:15 | Dianna M. Milewicz, University of Texas-Houston Medical School: Profibrillin-1 proteolytic processing: cellular regulation and human genetic variation |
| 9:35 | Daniel Constam, Harvard University: Axial defects in mice lacking SPC4/PACE4 |
| Reception in Wheeler Hall |
| Monday |
| 7:30-8:30 AM | Breakfast |
| Biochemistry and structural biology of proprotein and propeptide processing enzymes |
| Discussion leader: Richard Mains, University of Connecticut Health Center |
| 9:00 | Nabil Seidah, Clinical Research Institute of Montreal: Structure and function in processing of proprotein convertases |
| 9:30 | Iris Lindberg, Louisiana State University Medical Center: Endogenous and synthetic inhibitors of processing enzymes |
| 10:00 | Conference Photo |
| 10:30 | Break |
| 11:00 | Vivian Hook, UC San Diego: Novel protease and protease inhibitor components in regulated secretory vesicles of chromaffin cells |
| 11:30 | Tomoko Komiyama, University of Michigan Medical School: Engineering Eglin c for Inhibition of the Kexin/Furin Family of Proprotein Processing Proteases |
| 11:50 | Mario Amzel, Johns Hopkins University: 3 dimensional structure of PHM and its mechanistic implications |
| 12:30-1:30 | Lunch |
| 1:30-4:00 | Free Time |
| 4:00-6:00 | Poster Session I: last name A-K stand by posters 4-5:30 |
| 6:00 | Dinner |
| Newly emerging processing enzymes in physiology and disease |
| Discussion Leader: Bob Fuller, University of Michigan, Ann Arbor |
| 7:30 | Ann Wakefield, University of Oxford: The PRT1 protease gene family of the fungal pathogen Pneumocystis carinii |
| 8:00 | Susan Michaelis, Johns Hopkins University: Purification of the yeast a-factor integral membrane metalloprotease, Ste24p/Afc1p, provides direct evidence for its sufficiency to promote prenyl protein processing |
| 8:30 | Peter Espenshade, University of Texas Southwestern Medical Center: Transport Dependent Proteolysis: Control of Cholesterol Homeostasis by SREBPs |
| 9:00 | Dennis Selkoe, Harvard University Medical School: Intramembranous Proteolysis of APP and Notch and the Pathogenesis of Alzheimer's Disease |
| 9:30 | Robert Vassar, Amgen: b-secretase and APP processing |
| Tuesday |
| 7:30-8:30 AM | Breakfast |
| Structure and regulation in protein sorting and localization |
| Discussion Leader: Peter Arvan, Albert Einstein Medical College |
| 9:00 | Steve Nothwehr, University of Missouri: Mechanisms for localization of TGN proteolytic processing enzymes in yeast |
| 9:30 | Fred Maxfield, Cornell University Medical College: Determinants of protein traffic between endosomes and the Golgi |
| 10:00 | Gary Thomas, Vollum Institute: PACs1 and dynamic regulation of protein localization by a phosphorylation/dephosphorylation cycle |
| 10:30 | Break |
| 11:00 | Oleg Varlamov, Albert Einstein College of Medicine: The cytoplasmic tail of carboxypeptidase D binds protein phosphatase 2A: Implications for trafficking of TGN proteins |
| 11:20 | Linton Traub, Washington University, St. Louis: Clathrin-coatassembly: strength in numbers |
| 11:50 | Tommy Kirchhausen, Harvard University: Atomic structure of clathrin: a molecular explanation of how it sorts |
| 12:30-1:30 | Lunch |
| 1:30-4:00 | Free Time |
| 4:00-6:00 | Poster Session I: last name L-Z stand by posters 4-5:30 |
| 6:00 | Dinner |
| Novel features of secretory and processing compartments |
| Discussion Leader: Sharon Tooze, Imperial Cancer Research Fund |
| 7:30 | Brad Marsh and Kathryn Howell (speaker), University of Colorado Health Sciences Center: High resolution 3-D structure of the Golgi complex in pancreatic b cells |
| 8:00 | Todd Graham, Vanderbilt University: Drs2p, a P-type ATPase and potential lipid flippase implicated in clathrin function at the yeast TGN |
| 8:30 | Daryll DeWald, Utah State University: The Pik1p Phosphatidylinositol 4-kinase Regulates Secretion at the Golgi in Yeast |
| 9:00 | Betty Eipper, University of Connecticut Health Center: PAM: meeting the special needs of metalloenzymes |
| 9:30 | Judith Klumperman, University of Utrecht: Ultrastructural analysis of secretory protein sorting and concentration |
| Wednesday |
| 7:30-8:30 AM | Breakfast |
| Generation and maturation of secretory vesicles and granules |
| Discussion Leader: Iris Lindberg, Louisiana State University Medical Center |
| 9:00 | Y. Peng Loh, National Institutes of Health: Sorting of proinsulin to the regulated secretory pathway |
| 9:30 | Peter Arvan, Albert Einstein College of Medicine: Luminal protein sorting and precursor processing in the distal secretory pathway. |
| 10:00 | Priscilla Dannies, Yale University: Storing hormones in secretory granules: the process of hormone aggregation in cells |
| 10:20 | Kathleen Shennan, Institute of Medical Sciences, Aberdeen, Scotland, Involvement of the membrane lipid bilayer in sorting PC2 into the regulated secretory pathway |
| 10:40 | Break |
| 11:00 | Anirban Siddhanta (sub for Dennis Shields), Albert Einstein College of Medicine: Phospholipid biosynthesis and the generation of secretory vesicles |
| 11:30 | Sharon Tooze, Imperial Cancer Research Fund, London: Maturation of neuroendocrine secretory granules-the role of cytosolic components |
| 12:00 | Yan Feng, Harvard Institute of Chemistry and Cell Biology: Chemical genetics of membrane trafficking |
| 12:30-1:30 | Lunch |
| 1:30-4:00 | Free Time |
| 4:00-6:00 | Poster Session II: last name A-K stand by posters 4-5:30 |
| 6:00 | Dinner |
| New approaches to understanding peptide diversity |
| Discussion Leader: Betty Eipper, University of Connecticut Health Center |
| 7:30 | Baldomero Olivera, University of Utah: Diversity of Cone Snail Neuropeptides: Hypermutation and post-translational modification. |
| 8:00 | Jonathan Sweedler, University of Illinois: From Prohormone to Final Peptides: Single Cell Peptide Processing in Aplysia californica As Revealed by Mass Spectrometry |
| 8:30 | Sarah B. Pierce, Harvard University Medical School: INS-1, one of many insulin-related genes in C. elegans, can regulate dauer formation |
| 8:50 | Lloyd Fricker, Albert Einstein College of Medicine: Identification of novel peptides from carboxypeptidase E-deficient fat/fat mice: Implications for neuropeptide processing |
| 9:20 | Bob Day, University of Sherbrooke: Generation of functional diversity of neuropeptide precursors through differential processing by the subtilase-like pro-protein convertases (SPCs) |
| Thursday |
| 7:30-8:30 AM | Breakfast |
| The machinery and regulation of exocytosis |
| Discussion Leader: Kathryn Howell, University of Colorado Health Sciences Center |
| 9:00 | Hsiao-Ping Moore, University of California, Berkeley: Molecular steps involved in the assembly of functional secretory granules |
| 9:30 | Peter Novick, Yale University: Spatial Regulation of Exocytosis |
| 10:00 | John Hutton, University of Colorado Health Sciences Center: Regulated phosphorylation of dense core granule membrane proteins and insulin secretion |
| 10:30 | Break |
| 11:00 | David Zenisek, Vollum Institute: Transport, capture and exocytosis of single synaptic vesicles imaged at active zones of a giant synapse |
| 11:30 | Tom Martin, University of Wisconsin, Madison: Docking, priming and fusion of dense-core vesicles. |
| 12:30-1:30 | Lunch |
| 1:30-4:00 | Free Time |
| 4:00-5:30 | Poster Session II: last name L-Z stand by posters 4-5:30 |
| 5:30 | Business Meeting, all conferees should attend |
| 6:00 | Dinner |
| Genetic and physiology of processing II and late-breaking stories |
| 7:30 | Perspectives on meeting by Discussion Leader: Nabil Seidah, Clinical Research Institute of Montreal |
| 7:45 | Donald F. Steiner, University of Chicago: Lessons from PC Gene Disruptions and Mutations |
| Majambu Mbikay, Loeb Research Institute, Ottawa: Allele Transmission and Precursor Processing in a PC1/3 Mutant Mouse |
| 8:45 | Neil Taylor, University of Leuven, Belgium, The role of the proprotein convertase furin in pancreatic development and function |
| 9:05 | Virginie Laurent, Louisiana State University Medical Center: Recent progress on the 7B2 knock-out mouse |
| Party with entertainment (?!?!) by Fricker |
