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2:00 pm - 11:00 pm
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Arrival and registration
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6:00 pm
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Dinner
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7:30 am - 8:30 am
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Breakfast
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9:00 am
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Conference photo
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9:15 am
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Welcome: J. Stuart Nelson, Stefan Andersson-Engels
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Session Title: |
Animation and Video Sequences of Laser
Interactions with Biological Tissues
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Chair: Steven L. Jacques, Oregon Medical Laser Center
Session summary The dynamics of laser-tissue interactions and light
transport in biological tissues
can often be better conveyed visually by theory-based animation and an experimentally acquired
video sequences of images. A complex interaction can be better conveyed visually. This session
brings together investigators who have used animation and video sequences to convey the complexity
of laser effects to a general audience. The talks will include an introduction to the basic theory
underlying visual presentations.
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9:25 am - 9:30 am
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Steven L. Jacques, Oregon Medical Laser Center
"Introduction"
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9:30 am - 9:55 am
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Lihong V. Wang, Texas A&M University
"Laser pulse propagation in biological tissue"
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9:55 am - 10:10 am
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Discussion
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10:10 am - 10:35 am
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Rudolf Verdaasdonk, Utrecht Medical Center, The Netherlands
"Laser-tissue interactions with biological tissues revealed by real-time imaging techniques and
tissue modelling"
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10:35 am - 10:50 am
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Discussion
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10:50 am - 11:10 am
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Coffee break
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11:10 am - 11:35 am
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Duncan Maitland, Lawrence Livermore National Laboratory
"Photomechanical medical devices"
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11:35 am - 11:50 am
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Discussion
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11:50 am - 12:15 pm
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Joseph T. Walsh, Northwestern University
"Polarized light imaging of tissue: stress propagation in phantoms and tissue"
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12:15 pm - 12:30 pm
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Discussion
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12:30 pm - 1:30 pm
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Lunch
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1:30 pm - 6:00 pm
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Free of all formal activities
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6:00 pm
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Dinner
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Session Title: |
Optical Based Molecular Assays
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Chair: Stefan Seeger, Physical Chemistry Institute, Universitat Zurich, Switzerland
Session summary Users of modern in vivo diagnostic techniques demand accurate,
precise, and rapid quantification of specific molecular and particulate species. Systems must also meet numerous
sample processing and data handling needs. Both sample preparation and ultrasensitive detection will push
bioanalytical applications like clinical diagnostics and drug screening into new dimensions. This session will
provide a description of the various phenomena associated with laser interaction with blood and other body fluids.
Novel laser-based sample isolation and preparation with new detection methods such as multiphoton-spectroscopy and
single-molecule detection by laser-induced fluorescence will be discussed.
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7:30 pm - 7:35 pm
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Stefan Seeger, Physical Chemistry Institute, Universitat Zurich, Switzerland
"Introduction"
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7:35 pm - 8:05 pm
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Bert Hecht, ETH-Zentrum, Zurich, Switzerland
"Single molecular assays based on statistical interactions"
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8:05 pm - 8:20 pm
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Discussion
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8:20 pm - 8:50 pm
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Robert Bonner, National Institute of Child Health and Human Development
"Laser capture microdissection and the macromolecular analysis of specific cell populations within normal and
pathological tissues"
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8:50 pm - 9:05 pm
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Discussion
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9:05 pm - 9:35 pm
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Jason Shear, University of Texas at Austin
"Probing neurotransmitters using multiphoton-excited fluorescence"
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9:35 pm - 9:50 pm
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Discussion
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7:30 am - 8:30 am
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Breakfast
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Session Title: |
High Resolution Functional Imaging in Cells and Tissues: New Methods and Contrast Mechanisms
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| Chair: Bruce Tromberg,
Beckman Laser Institute and Medical Clinic, University of California, Irvine
Session summary When non-linear interactions are combined with analytical tools
capitalizing on the spectral, temporal, and coherence properties of light, microscopy methods are pushed beyond the
domain of simple image formation and into the regime of high-resolution functional analysis. At the core of this work
is the drive to characterize molecular composition and cellular structure on a spatial scale that is determined by
non-linear processes such as multi-photon fluorescence excitation and third harmonic generation. State-of-the-art
methods that combine computational models, optical spectroscopy, and imaging with specific molecular probes of
biological processes will be introduced. These methods can provide dramatic enhancements in functional
signal-to-noise ratios, resulting in sub-wavelength resolved images of structures in single cells at depths of up to
hundreds of microns beneath a tissue surface. Applications to single cell tomographic imaging will be shown that
demonstrate unique contrast elements based on principles of third harmonic generation. Studies of skin physiology and
tumor biology will be presented that utilize intravital imaging strategies.
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9:00 am - 9:05 am
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Bruce Tromberg, Beckman Laser Institute and Medical Clinic, University of California, Irvine
"Introduction"
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9:05 am - 9:35 am
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Jeff Squier, University of California, San Diego
"Third harmonic generation microscopy: a new technique for visualizing low contrast interfaces in biological
systems"
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9:35 am - 9:50 am
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Discussion
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9:50 am - 10:20 am
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Changhuei Yang and Adam Wax, Massachusetts Institute of Technology
"Interferometric Phase-Based Biomedical Measurements - Dialogue between a cell-biologist
and an optical engineer"
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10:20 am - 10:35 am
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Discussion
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10:35 am - 10:55 am
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Coffee break
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10:55 am - 11:25 am
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Peter So, Massachusetts Institute of Technology
"Microscopic diagnosis of tissue physiological states using two-photon spectroscopy"
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11:25 am - 11:40 am
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Discussion
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11:40 am - 12:10 pm
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Rakesh Jain, Harvard Medical School, Massachusetts General Hospital
"In vivo molecular and functional imaging of tumors: new insights from intravital microscopy"
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12:10 pm - 12:25 pm
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Discussion
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12:30 pm - 1:30 pm
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Lunch
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1:30 pm - 4:30 pm
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Free of all formal activities
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4:30 pm - 6:00 pm
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Poster Session
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6:00 pm
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Dinner
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Session Title: |
Biomedical Optics and Laser Treatment of Human Skin
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Chair: R. Rox Anderson, Harvard Medical School, Massachusetts General Hospital
Session summary Lasers are now the treatment of choice for several entities for
which no reliable or effective modality was previously available. However, in addition to many exciting and
beneficial advances in the clinical use of lasers in dermatology, there have been a disturbing number of unkept
promises and oversold applications. The objectives of this session are to: 1) identify recent scientific and
technologic advances in optical technologies as applied to human skin; 2) examine the limitations of current lasers
and define those problems that must be resolved to improve their clinical utility; 3) emphasize the important
contribution of basic optics research in the development of clinical applications using lasers; and 4) allow
participants to develop consensus that guides future research, product development and clinical applications. The
invited speakers comprise a cross-section of leaders in the field of biomedical optics and clinical practitioners.
Much of the research to be presented at this conference will be cutting edge, not previously peer-reviewed or
published.
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7:30 pm - 7:35 pm
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R. Rox Anderson, Harvard Medical School, Massachusetts General Hospital
"Introduction"
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7:35 pm - 8:00 pm
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David Goldberg, University of Medicine and Dentistry of New Jersey
"Non-ablative dermal remodeling"
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8:00 pm - 8:15 pm
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Discussion
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8:15 pm - 8:45 pm
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Dieter Manstein, Harvard Medical School, Massachusetts General Hospital
"Lasers and hair follicles"
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8:45 pm - 9:00 pm
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Discussion
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9:00 pm - 9:30 pm
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Allan Halpern, Sloan-Kettering Cancer Center
"Optical diagnosis of melanoma"
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9:30 pm - 9:45 pm
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Discussion
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9:45 pm - 10:00 pm
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Poster Discussion
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7:30 am - 8:30 am
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Breakfast
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Session Title: |
Optical Coherence Tomography and Speckle Interferometry
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| Chair: Alexander Knuettel, ISIS Optronics GmbH,
Mannheim, Germany
Session summary Tremendous advancements in OCT and speckle interferometry have
occurred over the past few years. The spatial resolution issue in OCT has come to a point where cell layers or even
cells can be delineated in tissue. Beyond morphology, new or refined approaches permit tissue characterization based
on inherent physical parameters. The goals of this session are to demonstrate very recent progress in trying to
establish non-invasive interferometry parallel to invasive biopsy applications. The partly complementary nature of
acquired data from interferometry and other microscopic methods will be an important feature of the session. Newest
results from non- and minimally-invasive clinical experimentation will demonstrate the degree of maturity for
industrial applications.
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9:00 am - 9:05 am
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Alexander Knuettel, ISIS Optronics GmbH, Mannheim, Germany
"Introduction"
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9:05 am - 9:35 am
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Guillermo Tearney, Harvard Medical School, Massachusetts General Hospital
"Optical coherence tomography in the clinic"
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9:35 am - 9:50 am
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Discussion
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9:50 am - 10:20 am
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Joseph Izatt, Case Western Reserve University, University Hospitals of Cleveland
"Functional optical coherence tomography"
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10:20 am - 10:35 am
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Discussion
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10:35 am - 10:55 am
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Coffee break
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10:55 am - 11:25 am
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Emmanuel Beaurepaire, Ecole Supérieure de Physique et Chimie Industrielles, Paris, France
"Tissue imaging with combined optical coherence and two-photon excitation microscopy"
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11:25 am - 11:40 am
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Discussion
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11:40 am - 12:10 pm
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Adrian Podoleanu, University of Kent at Canterbury, UK
"Advances in the simultaneous OCT and confocal imaging of the retina"
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12:10 pm - 12:25 pm
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Discussion
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12:30 pm - 1:30 pm
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Lunch
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1:30 pm - 4:30 pm
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Free of all formal activities
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Special Seminar: 4:30 pm - 6:00 pm |
Career Opportunities in Biomedical Optics
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| Chair: Michael S. Feld, Massachusetts Institute of Technology
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Session Title: |
Recent Developments in Photodynamic Therapy
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Chair: Willem Star,
Daniel den Hoed Cancer Centre, Rotterdam
Session summary This session will outline the principles of PDT and give a very brief
overview of its current applications and future potential leading into the three talks concerning potential
applications to the brain, skin, and eye. Brain malignancies represent an important target tissue for PDT because
enhanced local destruction of the tumor could significantly extend survival and improve quality of life. PDT of skin
lesions will explore the many recent developments in the use of aminolevulinic acid and its esters for malignant
tumors and psoriasis. One of the most exciting developments in PDT has been the realization that the technique can
be used to prevent the progression of age-related macular degeneration which is the major cause of blindness in the
developed world. This approach to macular degeneration will be discussed along with the most recent findings in terms
of new photosensitizing drugs, light sources and therapeutic opportunities.
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7:30 pm - 7:45 pm
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Willem Star,
Daniel den Hoed Cancer Centre, Rotterdam
"Recent results using fractionated topical ALA-PDT"
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7:45 pm - 8:15 pm
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Brian Wilson, Ontario Cancer Institute, Toronto, Canada
"Can photodynamic techniques help with the brain tumor problem?"
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8:15 pm - 8:30 pm
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Discussion
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8:30 pm - 9:00 pm
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Katarina Svanberg, Lund University Hospital, Sweden
"Photodynamic therapy of skin lesions using aminolevulinic acid - diagnostic measurements and clinical outcome"
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8:00 pm - 9:15 pm
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Discussion
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9:15 pm - 9:45 pm
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Reginald Birngruber, Medical Laser Center, Lübeck, Germany
"Photodynamic therapy of age related macula degeneration:
Principles and clinical applications"
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9:45 pm - 10:00 pm
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Discussion
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7:30 am - 8:30 am
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Breakfast
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Session Title: |
Inverse Problems and Statistical Methods in Optical Biology
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| Chair: Chair: Thomas Milner, University of Texas
at Austin
Session summary The non-invasive nature and ultra-wide signal bandwidth native to
optical methods are being increasingly utilized to investigate novel techniques for tissue diagnostics. Use of
recorded optical data to determine quantitative information characterizing a tissue invariably involves finding the
solution to an inverse problem. Because a solution can be extremely sensitive to measurement noise and is not
necessarily unique, inverse problems are difficult to solve and frequently require incorporation of additional
information. We will explore contemporary inverse problems in biomedical optics and spectroscopy that have
application to tissue diagnostics.
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9:00 am - 9:05 am
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Thomas Milner, University of Texas at Austin
"Introduction"
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9:05 am - 9:35 am
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Irene Georgakoudi and Michael Feld, Massachusetts Institute of Technology
"Fluorectification: inverting tissue spectra to extract diagnostic information - a Socratic dialog"
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9:35 am - 9:50 am
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Discussion
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9:50 am - 10:20 am
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Rebecca Richards-Kortum, University of Texas at Austin
"Cervical tissue autofluorescence: what the models don't tell us"
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10:20 am - 10:35 am
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Discussion
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10:35 am - 10:55 am
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Coffee break
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10:55 am - 11:25 am
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Andreas Hielscher, SUNY Health Sciences Center
"The inverse problem in optical tomographic imaging"
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11:25 am - 11:40 am
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Discussion
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11:40 am - 12:10 pm
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Scott Prahl, Oregon Medical Laser Center
"Inverse diffusion problems--an oxymoron?"
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12:10 pm - 12:25 pm
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Discussion
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12:30 pm - 1:30 pm
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Lunch
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1:30 pm - 4:30 pm
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Free of all formal activities
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4:30 pm - 6:00 pm
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Poster Session
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6:00 pm
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Dinner
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Session Title: |
35 Years of Research and Development and 20
Gordon Research Conferences on Lasers in Medicine and Biology: What Can We Learn from the Past and What Do We Expect
for the Future?
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Chairs: Franz Hillenkamp, Institute for Medical Physics & Biophysics, University of
Muenster, Germany and Myron Wolbarsht, Duke University
Session summary Only two to three years after the first lasers were realized in
physics and electronics laboratories were the first medical applications suggested and attempted. Early on, many
medical applications were guided by wishes (lasers can heal melanoma!) rather than an understanding of the properties
of laser radiation and its interaction with cells and tissue. The series of Gordon Research Conferences on Lasers in
Medicine and Biology which started in the mid-60's have played a key role in putting such medical applications in
proper perspective and guiding scientists to conceptualize new ones. Even though the majority of participants have
always been natural scientists and engineers, it was the mix of physicians, biologists, (bio)chemists and the strong
international participation which generated the genuinely stimulating innovative and productive atmosphere at these
conferences. This session intends to recapture some of the early enthusiasm by reflecting on those developments which
have successfully passaged from bench to bedside. We will also analyze those endeavors which remain stuck in the
trenches of basic research and engineering perfection as well as commemorating the not too few ones which have fallen
by the wayside.
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7:30 pm - 7:50 pm
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Franz Hillenkamp, Institute for Medical Physics & Biophysics, University of Muenster, Germany
"Introduction"
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7:50 pm - 8:10 pm
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A. J. Welch, University of Texas at Austin
"Thermal response of tissue to laser irradiation"
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8:10 pm - 8:20 pm
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Discussion
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8:20 pm - 8:40 pm
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Carmen Puliafito, New England Eye Center
"Lasers and the eye: a paradigm for technology transfer"
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8:40 pm - 8:50 pm
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Discussion
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8:50 pm - 9:10 pm
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R. Rox Anderson, Harvard Medical School, Massachusetts General Hospital
"Lasers in dermatology: past and future"
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9:10 pm - 9:20 pm
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Discussion
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9:20 pm - 9:40 pm
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Myron Wolbarsht, Duke University
"Prowling through past programs: successes and failures"
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9:40 pm - 9:50 pm
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Discussion
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7:30 am - 8:30 am
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Breakfast
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Session Title: |
Imaging and Spectroscopy of Deep Tissue with Diffuse Light
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| Chair: Arjun Yodh, University of Pennsylvania
Session summary Application of near-infrared (NIR) optical methods for in-vivo
imaging and spectroscopy of deep tissues appear feasible with levels of specificity and resolution superior to early
work as a result of the separation of scattering and absorption afforded by the diffusion approximation. NIR optical
methods have several unique measurable parameters including, but not limited to, blood dynamics, blood volume, blood
oxygen saturation, tissue water and fat content, and cell organelle concentration and size. In this session, we focus
on applications of these tools for brain and breast characterization, and tissue diagnostics in PDT and esophageal
cancer.
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9:00 am - 9:05 am
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Arjun Yodh, University of Pennsylvania
"Introduction"
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9:05 am - 9:35 am
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David Boas, Harvard Medical School, Massachusetts General Hospital
"Diffuse optical tomography of brain: difference versus baseline imaging?"
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9:35 am - 9:50 am
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Discussion
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9:50 am - 10:20 am
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Michael Patterson, Hamilton Regional Cancer Center, Canada
"Can multiply scattered light provide useful information for photodynamic therapy dosimetry?"
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10:20 am - 10:35 am
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Discussion
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10:35 am - 10:55 am
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Coffee break
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10:55 am - 11:25 am
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Joe Culver, University of Pennsylvania
"Large, rapid data sets for 3D diffuse optical mammography"
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11:25 am - 11:40 am
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Discussion
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11:40 am - 12:10 pm
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Lev Perelman, Massachusetts Institute of Technology
"Light scattering spectroscopy for probing epithelial tissues"
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12:10 pm - 12:25 pm
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Discussion
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