Saturday
1:00 pm - 5:00 pm
Arrival and Check-in
3:00 pm - 3:15 pm
Introductory Comments by GRC Staff / Welcome and Introduction from the Chairs
3:15 pm - 4:00 pm
Membrane Proteins in Disease
Discussion Leader: Remo Bättig (École polytechnique fédérale de Lausanne (EPFL), Switzerland)
3:15 pm - 3:25 pm
Austin Tedman (Indiana University, United States)
"Ribosomal Frameshifting Selectively Modulates the Expression, Assembly, and Function of a Misfolded CFTR Variant"
3:25 pm - 3:30 pm
Discussion
3:30 pm - 3:40 pm
Kathryn Brewer (Vanderbilt University, United States)
"Assessing KCNQ1 Stability and its Role in Long QT Syndrome"
3:40 pm - 3:45 pm
Discussion
3:45 pm - 3:55 pm
Andrew Molina (University of Chicago, United States)
"Uncovering the Mechanism of Potassium Channel Folding and Assembly"
3:55 pm - 4:00 pm
Discussion
4:00 pm - 5:30 pm
Poster Session
5:30 pm - 7:30 pm
Probing Membrane Protein Folding with Computational Approaches and Empirical Screening
Discussion Leader: Samyuktha Ramesh (The Walter and Eliza Hall Institute, Australia)
5:30 pm - 5:40 pm
Antoniya Aleksandrova (National Institute of Neurological Disorders and Stroke, NIH, United States)
"Rules of Interaction: Contributions of Symmetry in Membrane Protein Function and Assembly"
5:40 pm - 5:45 pm
Discussion
5:45 pm - 5:55 pm
Daniel Montezano (University of Kansas, United States)
"Contacts Reveal the Barrel"
5:55 pm - 6:00 pm
Discussion
6:00 pm - 6:10 pm
Dane Evan Zambrano (University of Washington Department of Medicine, Biochemistry, United States)
"De Novo Design of Hybrid Protein-Lipid Nanomaterials"
6:10 pm - 6:15 pm
Discussion
6:15 pm - 6:25 pm
Karen Noguera (Indiana University, United States)
"Probing Endoplasmic Reticulum Membrane Protein Complex (EMC) Recognition by Deep Mutational Scanning"
6:25 pm - 6:30 pm
Discussion
6:30 pm - 6:40 pm
Katherine Stefanski (Vanderbilt University, United States)
"High-Throughput Screening to Identify Modulators of Membrane Protein Raft Affinity in Giant Plasma Membrane Vesicles"
6:40 pm - 6:45 pm
Discussion
6:45 pm - 6:55 pm
Lucas Rudden (EPFL, Switzerland)
"Computational Design of Multi-Domain Receptors with Programmable Sensing and Signaling Functions"
6:55 pm - 7:00 pm
Discussion
7:00 pm - 7:10 pm
Ben Hardy (University of Bristol, United Kingdom)
"Computational Design and Cellular Production of a De Novo Transmembrane Cytochrome"
7:10 pm - 7:15 pm
Discussion
7:15 pm - 7:25 pm
Donovan Trinidad (University of California, San Francisco, United States)
"Exploring the Structure Function Landscape of the Human Sodium–Taurocholate Co-Transporting Polypeptide (NTCP)"
7:25 pm - 7:30 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Sunday
7:30 am - 8:30 am
Breakfast
9:00 am - 11:00 am
Effects of Lipids and Physical Forces on Membrane Proteins
Discussion Leader: Mason Wilkinson (Vanderbilt University, United States)
9:00 am - 9:10 am
Brandon Dzuba (University of Colorado Boulder, United States)
"Towards High-Throughput AFM-based Force-Spectroscopy Studies of Membrane Proteins Using Site-Specific Attachment"
9:10 am - 9:15 am
Discussion
9:15 am - 9:25 am
Abigail Schroeter (University of Chicago, United States)
"Using HDX-MS To Probe The Dynamics and Denatured State of GlpG, a Helical Membrane Protein"
9:25 am - 9:30 am
Discussion
9:30 am - 9:40 am
Melanie Ernst (Washington University in St. Louis, United States)
"Mapping Out the Physical Driving Forces of Functional Assembly of the Fluoride Channel Fluc in Membranes"
9:40 am - 9:45 am
Discussion
9:45 am - 9:55 am
Aamna Jain (Saarland University, Germany)
"Isolation of Endoplasmic Reticulum Subdomains from Mammalian Cells"
9:55 am - 10:00 am
Discussion
10:00 am - 10:10 am
Justin Westerfield (Stockholm University, Sweden)
"Cotranslational Membrane Insertion of the Voltage-Gated Potassium Channel KvAP"
10:10 am - 10:15 am
Discussion
10:15 am - 10:25 am
Yein Christina Park (NHLBI/Johns Hopkins, United States)
"Lipid Bilayer Thickness Modulates the Folding Stability of Outer Membrane Phospholipase A"
10:25 am - 10:30 am
Discussion
10:30 am - 10:40 am
Kathryn Diederichs (National Institutes of Health, United States)
"Towards Mechanistic Understanding of Mitochondrial beta-barrel Biogenesis: Structural Studies of the Sorting and Assembly Machinery"
10:40 am - 10:45 am
Discussion
10:45 am - 10:55 am
Daniel Williams-Jones (University of Oxford, United Kingdom)
"Tuneable Force-Transduction through the Escherichia Coli Periplasm"
10:55 am - 11:00 am
Discussion
11:00 am - 12:30 pm
Poster Session
Coffee will be served in the poster area from 11:00 am - 11:30 am
12:30 pm - 1:30 pm
Lunch
1:30 pm - 2:30 pm
Mentorship Component: Careers in Membrane Protein Folding
Discussion Leader: Alexis Reyes (UCSD, United States)
1:30 pm - 2:30 pm
Panel Discussion
Q&A Panel for Early Career Scientists
Heedeok Hong (Michigan State University, United States)
Raymond Sierra (LCLS Sample Environment and Delivery, United States)
Jonathan Schlebach (Indiana University, Bloomington, United States)
Cristina Risueno (Sibylla Biotech, Italy)
Alberto Boldrini (Sibylla Biotech S.p.A., Italy)
2:30 pm - 3:00 pm
Evaluation Period
Complete the GRS Evaluation Forms; Election of Future Chair(s)
3:00 pm
Seminar Concludes