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Sunday evening, June 6
Myosin and kinesin: high resolution structures and mechanism
7:30 Roger Goody, Chair; Kathy Trybus, Poster/Discussion Chair
7:30 Welcome Sarah Hitchcock-DeGregori and GRC Staff
7:50 Roger Goody
Introduction
8:00 Eckhard Mandelkow
Structure of kinesin and interactions with microtubules
8:25 Anne Houdusse
Aree conformational states of scallop myosin S1 fragment by X-ray crystallography
8:50 Discussion
Kathy Trybus Evidence for the pre-power stroke
Ken Holmes
Monday morning, June 7
Single molecule studies of myosin and other motors
9:00 David Warshaw, Chair; Toshio Yanagida, Poster/Discussion Chair
9:00 David Warshaw
Molecular mechanisms and mechanics of muscle myosins
9:25 Justin Molloy
Mechanical events observed during the cross-bridge cycles of myosins I, II and V
9:50 Kazuhiko Kinosita
Matsubara Honorary Lecture
A rotary motor that can work at near 100% efficiency
10:15 Coffee break and photograph
11:00 Discussion
Toshio Yanagida Processive movement of single one-headed motors
Joe Howard
Jim Sellers
Monday afternoon, June 7
4:30 Reception and Poster Session
Monday evening, June 7
Myosin, kinesin and other motors
7:30 David Hackney, Chair; Sharyn Endow, Poster/Discussion Chair
7:30 David Hackney
Where's the stator?
7:55 Nobutaka Hirokawa
A processive monomeric motor KIF 1A: its motility, structure andjunction
8:20 Mchael Ostap
Relating chemistry tofiniction: myosin-I, 11, and V
8:45 Discussion
Sharyn Endow Kinesin motors: polarity and motility
Edwin Taylor
Tuesday morning, June 8
Actin filament dynamics
9:00 Emil Reisler, Chair; Clarence Schutt, Poster/Discussion Chair
9:00 Emil Reisler
Dynamic changes at the suhdomains 211 interface in F-actin
9:25 Ed Egelman
FA,f studies of F-actin structural states and cooperativity
9:50 Marie-France Carlier
Control of actin dynamics and movement hy regulatory proteins: Arp213 complex, actin
depolymerizingjactor, andprofilin
10:15 Dorit Hanein
Conformational variahility in actomyosin complexes
10:40 Coffee Break
11:10 Discussion
Clarence Schutt
Tom Pollard
Tuesday afternoon, June 8
4:30 Afternoon at the movies
Tuesday evening, June 8
Thin filament regulation: structure
7:30 Michael Geeves, Chair; John Squire, Poster/Discussion Chair
7:30 Michael Geeves
Introduction
7:40 Brian Sykes
AMR structural and dynamics studies of the interaction of T11C and TnI
8:05 Takeyuki Wakabayashi
Calcium-induced relocation of tropomyosin-troponin on actinfilaments revealed by EA/1
8:30 Discussion
John Squire The polite regulatory proteins tropomyosin and troponin: "After you! ", "No. After you!
Zenon Grabarek
Bill Lehman
Jill Trewhella
Wednesday morning, June 9
Muscle fiber studies: the intact actomyosin system
9:00 Roger Cooke, Chair; Yale Goldman, Poster/Discussion Chair
9:00 Roger Cooke
Thefraction of myosin heads strongly bound to actin in active muscle, monitored by
pyrene fluorescence
9:25 Yale Goldman
Myosin structural dynamics byfluorescence polarization
9:50 Bernhard Brenner
Cross-bridge attachment during isometric contraction probed using 2D X-ray diffraction
10:15 Vincenzo Lombardi
X-ray diffraction of single musclefibers: theftaction of myosin heads attached to actin
and the unitary step spectroscopy
10:40 Coffee Break
11:10 Discussion
Yale Goldman
Robert Simmons
Dave Thomas
Wednesday afternoon, June 9
4:30 Poster Session
Wednesday evening, June 9
Analysis of engineered proteins in living fibers and cells
7:30 Donald Winkelmann, Chair; David Maugham, Poster/Discussion Chair
7:30 Donald Winkelmann
Assembly and activity of GFP-tagged myosin in live muscle cells
7:55 Lee Sweeney
Using viral gene transfer to assess protein junction in skeletal muscle
8:20 Jeff Robbins
Transgenic modeleing of cardiac musclefunction
8:45 Discussion
David Maugham
Thursday morning, June 10
Thin filament activation: from molecules to fibers
9:00 Earl Homsher, Chair; Joseph Chalovich, Poster/Discussion Chair
9:00 Earl Homsher
Isometricforce and sliding speed measurements of isolated regulated thin filaments
9:25 Michael Geeves
Cooperativity in thinfilament regulation
9:50 Shin'ichi Ishiwata
Regulation mechanism as revealed using a thinfilament-reconstituted contractile system
10:15 Al Gordon
Thin filament regulation in skinned fibers and in vitro motility systems
10:40 Coffee break
11:10 Discussion
Joe Chalovich Regulation by troponin-tropomyosin of the transition between non-activating (weak) and activating (strong) states of myosin
Roger Craig
Sarah Hitchcock-DeGregori
Fernando Reinach
Thursday afternoon, June 10
5:00 Business Meeting
Thursday evening, June 10, 1999
Muscle at the new millennium: summary and review session
7:30 Sarah Hitchcock-DeGregori, Chair
7:30 Sarah Hitchcock-DeGregori Introduction
7:40 Hugh Huxley
8:05 Edwin Taylor
8:30 Fernando Reinach
8:55 Tom Pollard
9:30 Party
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