Evening Session
I. MOLECULAR STRUCTURE OF E-C COUPLING COMPONENTS
Leaders:
David Stokes
Terry Wagenknecht
| Time |
Speaker |
|
Topic |
|
| 19:45-20:00 |
Terry Wagenknecht |
|
Introduction to the structural basis of e-c coupling. |
| 20:00-20:30 |
Eduardo Perozo |
|
Molecular basis of proton-dependent activation gating in KcsA. |
| 20:30-21:00 |
Angela Dulhunty |
|
Structure of an RyR binding domain on the skeletal DHPR II-III loop:
consequences for activation or inhibition of RyR. |
| 21:00-21:30 |
Wah Chiu |
|
Electron cryomicroscopy of skeletal muscle
Ca2+ release channels in different states. |
| 21:30-22:00 |
ChulHee Kang |
|
Structure of calsequestrin: implications for calcium regulation. |
| 22:00-22:30 |
David Stokes |
|
Structure and dynamics of the Ca2+ ATPase by
electron microscopy. |
|
Morning Session
II. GENETIC DISEASE RESULTING FROM DEFECTS IN E-C COUPLING PROTEINS
Leaders:
Frank Lehmann-Horn
Charles Louis
| Time |
Speaker |
|
Topic |
|
| 9:00-9:15 |
Frank Lehmann-Horn |
|
News and views in channelopathies. |
| 9:15-9:45 |
Karin Jurkat-Rott |
|
A second gene for hypokalemic periodic paralysis. |
| 9:45-10:15 |
Tommie McCarthy |
|
Malignant hyperthermia susceptibility and central core disease: what
causes the difference. |
| 10:15-10:45 |
Coffee break and group picture. |
| 10:45-11:15 |
Jacques Veerkamp |
|
Alterations in native and cultured muscle of Brody disease patients. |
| 11:15-11:30 |
David MacLennan |
|
Molecular genetics of Brody disease. |
| 11:30-12:00 |
Steve Goldstein |
|
Channels involved in long QT syndrome and ventricular fibrillation. |
| 12:00-12:30 |
Poster highlights. |
|
|
III. STRUCTURE-FUNCTION ANALYSIS OF RYANODINE RECEPTORS
Leaders:
Jianje Ma
Wayne Chen
| Time |
Speaker |
|
Topic |
|
| 19:00-19:15 |
Jianjie Ma |
|
Limits and difficulties in the functional expression of ryanodine receptors
in heterologous cells |
| 19:15-19:45 |
GuoGuang Du |
|
The use of chimera formation and site-directed mutagenesis to analyze
structure/function relationships in the ryanodine receptor. |
| 19:45-20:15 |
Manjunatha Bhat |
|
Membrane topology and localization of ryanodine receptor calcium release
channel. |
| 20:15-20:45 |
Gerhard Meissner |
|
Probing the ion conducting pore of the Ca2+
release channel. |
| 20:45-21:15 |
Gregory Mignery |
|
InsP3 receptor structure and function: "Pore Progress". |
| 21:15-21:45 |
Wayne Chen |
|
Isoform-specific formation of heteromeric ryanodine receptor
Ca2+ release channels. |
| 21:45-22:15 |
Poster highlights. |
|
|
Morning Session
IV. INTERACTIONS BETWEEN RYANODINE RECEPTORS AND
alpha1-DIHYDROPYRIDINE RECEPTORS
Leaders:
Paul Allen
Clara Franzini-Armstrong
| Time |
Speaker |
|
Topic |
|
| 09:00-09:30 |
Clara Franzini-Armstrong |
|
DHPR-RyR: spatial relationships. |
| 09:30-10:00 |
Kurt Beam |
|
Bi-directional signalling between DHPRs and RyRs. |
| 10:00-10:30 |
Paul Allen |
|
EC-Coupling, orthograde and retrograde signalling through RyR1. |
| 10:30-11:00 |
coffee break |
| 11:00-11:30 |
Noriaki Ikemoto |
|
Peptide probe study of the signal transmission mechanism. |
| 11:30-12:00 |
Hector Valdivia |
|
Activation of ryanodine receptors by Imperatoxin A and a peptide segment
of the II-III loop of the dihydropyridine receptor. |
| 12:00-12:30 |
Bernhard Flucher |
|
Molecular domains involved in targeting and modulation of the skeletal
muscle DHP-Receptor. |
|
|
V. MODULATION OF RYANODINE RECEPTOR FUNCTION BY ACCESSORY PROTEINS AND BY
PROTEIN MODIFICATION
Leaders:
Susan Hamilton
Andy Marks
| Time |
Speaker |
|
Topic |
|
| 19:30-20:00 |
Andy Marks |
|
The ryanodine receptor as a macromolecular complex. |
| 20:00-20:30 |
Sidney Fleischer |
|
Ryanodine receptors and FK-Binding Proteins: Isoforms, association and
function. |
| 20:30-21:00 |
Susan Hamilton |
|
Functional domains of RyR1. |
| 21:00-21:30 |
Charles Louis |
|
Isoform-specific regulation of ryanodine receptors by calmodulin and
dantrolene. |
| 21:30-22:00 |
Francesco Zorzato |
|
Molecular and functional characterization of junctional sarcoplasmic
reticulm proteins. |
| 22:00-22:30 |
Meredith Bond |
|
A-kinase anchoring protein targeting of PKA to junctional sarcoplasmic
reticulum in cardiac muscle. |
|
|
Morning Session
VI. MODULATION OF RYANODINE RECEPTOR FUNCTION BY TRANSMEMBRANE GRADIENTS
AND PHARMACOLOGICAL AGENTS
Leaders:
Isaac Pessah
Alan Williams
| Time |
Speaker |
|
Topic |
|
| 09:00-09:30 |
Isaac Pessah |
|
Variations in sensitivity of Ryr isoforms to physiologic and pharmacologic
modulators. |
| 09:30-10:00 |
Graham Lamb |
|
Effects of SR Ca2+loading level and cytoplasmic
factors on Ca2+ release by depolarization or caffeine
in skinned fibers. |
| 10:00-10:30 |
Sandor Gyorke |
|
Ca2+ regulation of ryanodine receptors. |
| 10:30-11:00 |
coffee break |
| 11:00-11:30 |
Poster highlights |
| 11:30-12:00 |
Cecilia Hidalgo |
|
Effects of SH oxidation on the regulation of ryanodine receptor function
by Ca2+ and Mg2+. |
| 12:00-12:30 |
Alan Williams |
|
The interaction of novel ryanoids with the single RyR channels. |
|
|
VII. ELEMENTARY EVENTS OF Ca2+ RELEASE
Leaders:
Eduardo Rios
Gil Weir
| Time |
Speaker |
|
Topic |
|
| 19:30-19:45 |
Eduardo Rios |
|
Unanswered questions about Ca2+ sparks |
| 19:45-20:15 |
Ernst Niggli |
|
Cellular and subcellular refractoriness of
Ca2+-release in cardiac muscle. |
| 20:15-20:45 |
Heping Cheng |
|
Coupling of single L-type channels to Ca2+
sparks in heart. |
| 20:45-21:15 |
Ian Parker |
|
Puffs, blips, sparks and quarks: comparative anatomy of elementary
Ca2+ events. |
| 21:15-21:45 |
Chris Ward |
|
Modulation of local Ca2+ release events in
skeletal muscle. |
| 21:45-22:15 |
Adom Gonzalez |
|
Events induced by Ca release agonists in skeletal muscle. Insights from
video-rate scanning microscopy. |
| 22:15-22:30 |
Poster highlights |
|
Michael Klein |
|
How many Ca2+ sparks can be released in a single
sarcomere? |
|
|
Morning Session
VIII. PHYSIOLOGICAL Ca2+ RELEASE AND REUPTAKE
Leaders:
Knox Chandler
Donald Bers
| Time |
Speaker |
|
Topic |
|
| 09:00-09:45 |
Don Bers |
|
Integrated Ca2+ management in heart cells. |
|
Poster highlights |
|
Godfrey Smith |
|
Effects of reduced [ATP] on spontaneous
Ca2+ release in permeabilized single cardiac cells |
| 09:45-10:15 |
Mike Stern |
|
Local control theory in cardiac muscle. |
| 10:15-10:45 |
coffee break |
| 10:45-11:15 |
Steve Baylor |
|
Ca2+ release and spread within the sarcomere
of intact skeletal muscle fibers. |
| 11:15-12:00 |
Natalia Shirokova |
|
Control of Ca2+ release in mammalian skeletal
muscle. |
|
Poster highlights |
|
Carlo Caputo |
|
Potentiation of Ca2+ release affects its
Ca2+ dependent inactivation. |
| 12:00-12:30 |
David Allen |
|
Ca2+ release,
Ca2+ stores and muscle fatigue. |
|
|
IX. RyR3, IP3R AND OTHER E/C COUPLING PROTEINS
IN STRIATED MUSCLE
Leaders:
Barbara Block
Yasuo Ogawa
| Time |
Speaker |
|
Topic |
|
| 19:30-20:00 |
Yasuo Ogawa |
|
Different contributions between alpha-RyR and beta-RyR to CICR activity
in sarcoplasmic reticulum vesicles from frog skeletal muscles. |
| 20:00-20:15 |
Barbara Block |
|
Fiber type specific ryanodine receptors in fish. |
| 20:15-20:45 |
Vincenzo Sorrentino |
|
Functional properties and mechanisms of subcellular localization of RyR3
channels. |
| 20:45-21:15 |
Hiroshi Takeshima |
|
Mitsugumins - new protein components of the triad junction. |
| 21:15-21:45 |
Masamitsu Iino |
|
Structure-function relationships in the IP3 receptor. |
| 21:45-22:15 |
Enrique Jaimovich |
|
Function and subcellular localization of IP3R in muscle cells. |
|
|
|
