Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Site Staff / Welcome from the GRC Chair
7:40 pm - 9:30 pm
Keynote Session: Fundamental Mechanisms of Mutagenesis
Discussion Leader: Nuria Lopez-Bigas (IRB Barcelona, Spain)
7:40 pm - 8:20 pm
Sue Jinks-Robertson (Duke University Medical Center, United States)
"Topoisomerases and Their Distinctive Mutation Signatures"
8:20 pm - 8:35 pm
Discussion
8:35 pm - 9:15 pm
Reuben Harris (HHMI & UT Health San Antonio, United States)
"APOBEC Mutagenesis in Cancer - Molecular Mechanism and Translational Opportunities"
9:15 pm - 9:30 pm
Discussion
Monday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Understanding Mutation Signatures in Cancer and Aging
Discussion Leader: K.J. Patel (MRC Weatherall Institute of Molecular Medicine, United Kingdom)
9:00 am - 9:20 am
Nuria Lopez-Bigas (IRB Barcelona, Spain)
"Tumor Genomes Shed Light Into DNA Damage, Repair and Mutational Processes"
9:20 am - 9:30 am
Discussion
9:30 am - 9:50 am
Ruben van Boxtel (Princess Máxima Center for Pediatric Oncology, The Netherlands)
"Tracking Mutational Footprints and Clonal Dynamics in Human Hematopoiesis"
9:50 am - 10:00 am
Discussion
10:00 am - 10:10 am
Mia Petljak (Broad Institute of MIT and Harvard, United States)
"Mechanisms of APOBEC3 Mutagenesis in Human Cancer Cells"
10:10 am - 10:15 am
Discussion
10:15 am - 10:25 am
Steven Roberts (Washington State University, United States)
"Insertion and Deletion Mutagenesis Induced by APOBEC3A and APOBEC3B"
10:25 am - 10:30 am
Discussion
10:30 am - 11:00 am
Coffee Break
11:00 am - 11:20 am
Polina Shcherbakova (Eppley Institute for Research in Cancer and Allied Diseases, United States)
"Mechanisms of DNA Polymerase Episilon-Driven Genomic Instability in Cancers"
11:20 am - 11:30 am
Discussion
11:30 am - 11:40 am
Martin Reijns (MRC Human Genetics Unit, United Kingdom)
"Transcription-Associated Mutagenesis by Topoisomerase 1 in Cancer and the Germline"
11:40 am - 11:45 am
Discussion
11:45 am - 11:55 am
Lisa Casimir (Universite de Sherbrooke, Canada)
"Mutational Signatures of Genotoxic Exposures in Human Cells"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:20 pm
Martin Taylor (MRC Human Genetics Unit, University of Edinburgh, United Kingdom)
"Mutagenic DNA Repair and the Signatures of Lesion Bypass in Transcription and Replication"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
3:00 pm - 4:00 pm
The GRC Power Hour™
The GRC Power Hour™ is designed to address diversity and inclusion in the scientific workplace by providing a safe environment for informal and meaningful conversations amongst colleagues of all career stages. The program supports the professional growth of all members of our communities, including ethnicity, race and/or gender identity by providing an open forum for discussion and mentoring.
Organizers: Hannah Klein (New York Grossman University School of Medicine, United States) and Susan Lovett (Brandeis University, United States)
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Mutagenesis Arising From Replication Errors
Discussion Leader: Susan Lovett (Brandeis University, United States)
7:30 pm - 7:50 pm
Thomas Kunkel (National Institute of Environmental Health Sciences, NIH, United States)
"The Contribution of Extrinsic Proofreading to Eukaryotic Nuclear DNA Replication Fidelity"
7:50 pm - 8:00 pm
Discussion
8:00 pm - 8:20 pm
Roger Woodgate (National Institutes of Health, United States)
"Active Site Mutants of E.coli DNA Polymerase III with Altered Base and Sugar Selectivity"
8:20 pm - 8:30 pm
Discussion
8:30 pm - 8:40 pm
Pierre Murat (MRC Laboratory of Molecular Biology, United Kingdom)
"The Mutagenic Footprint of DNA Replication Initiation"
8:40 pm - 8:45 pm
Discussion
8:45 pm - 8:55 pm
Daria Ostroverkhova (Queen's University, Canada)
"Specific Patterns of Mutations Induced by Mutated DNA Polymerase Epsilon in Endometrial Cancer Genomes"
8:55 pm - 9:00 pm
Discussion
9:00 pm - 9:20 pm
David Pellman (Dana-Farber Cancer Insitute / Harvard Medical School, United States)
"Mechanisms Driving Rapid Genome Evolution"
9:20 pm - 9:30 pm
Discussion
Tuesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
DNA Damage Tolerance at the Replication Fork
Discussion Leader: Sarah Lambert (Institut Curie / CNRS, France)
9:00 am - 9:20 am
Susan Lovett (Brandeis University, United States)
"Template-Switch Mutagenesis by Protein DNA Crosslinks"
9:20 am - 9:30 am
Discussion
9:30 am - 9:50 am
Mark Hedglin (The Pennsylvania State University, United States)
"Re-Thinking the Replication of DNA Lesions: What Happens when a DNA lesion is Encountered in A Lagging Strand Template?"
9:50 am - 10:00 am
Discussion
10:00 am - 10:10 am
Sarah McMillan (University of Wisconsin - Madison, United States)
"Characterizing DnaC Replication Restart Bypass Variants"
10:10 am - 10:15 am
Discussion
10:15 am - 10:25 am
Youri Pavlov (Eppley Institute for Research in Cancer, University of Nebraska Medical Center, United States)
"Minimal Mutasome in Yeast"
10:25 am - 10:30 am
Discussion
10:30 am - 11:00 am
Group Photo / Coffee Break
11:00 am - 11:20 am
Wei Yang (National Institutes of Health, United States)
"Is Fork Reversal Post-Replicational?"
11:20 am - 11:30 am
Discussion
11:30 am - 11:40 am
Mai Tran (Tufts University, United States)
"SLX1/SLX4 Endonuclease is Important for DNA Damage Tolerance in the Absence of Translesion Synthesis"
11:40 am - 11:45 am
Discussion
11:45 am - 11:55 am
Maximilian Donsbach (Ludwig Maximilians Universität München, Germany)
"The FANCJ Helicase Remodels DNA-Protein Crosslinks to Promote Their Cleavage by SPRTN"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:20 pm
Dana Branzei (FIRC Institute for Molecular Oncology Foundation, Italy)
"To Switch or Not to Switch: The Choice Between Homologous Recombination and Mutagenic Bypass"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Visualizing Mutagenic Mechanisms
Discussion Leader: Wei Yang (National Institutes of Health, United States)
7:30 pm - 7:50 pm
Joseph Loparo (Harvard Medical School, United States)
"Finding Their Way: How Error-Prone Polymerases Gain Access to the Replication Fork"
7:50 pm - 8:00 pm
Discussion
8:00 pm - 8:20 pm
Joseph Yeeles (MRC Laboratory of Molecular Biology, United Kingdom)
"Priming in the Eukaryotic Replisome"
8:20 pm - 8:30 pm
Discussion
8:30 pm - 8:40 pm
Edwin Antony (Saint Louis University School of Medicine, United States)
"cryoEM Structures Reveal an Intrinsic Allostery in Rad52 that Mediates Rad51 Nucleation on RPA-Coated ssDNA in Homologous Recombination"
8:40 pm - 8:45 pm
Discussion
8:45 pm - 8:55 pm
Claudia Aloisi (Ecole Normale Superieure, France)
"Single-Molecule Fluorescence and Nanomanipulation to Characterize the Facilitated Recruitment of DNA Strand Break Repair by the PARP System"
8:55 pm - 9:00 pm
Discussion
9:00 pm - 9:20 pm
David Rueda (Imperial College London, United Kingdom)
"Searching and Finding Targets on Genomes"
9:20 pm - 9:30 pm
Discussion
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Repeat and Barrier-Induced Mutagenesis
Discussion Leader: Hannah Klein (New York Grossman University School of Medicine, United States)
9:00 am - 9:20 am
Marcel Tijsterman (Leiden University Medical Center, The Netherlands)
"Mutagenicity of Persistent Replication Fork Barriers"
9:20 am - 9:30 am
Discussion
9:30 am - 9:50 am
Catherine Freudenreich (Tufts University, United States)
"Mechanisms of Chromosome Fragility at Structure-Forming DNA Sequences"
9:50 am - 10:00 am
Discussion
10:00 am - 10:10 am
Kara Pytko (Pennsylvania State University, United States)
"Characterization of Human DNA Polymerase Delta-Mediated Replication of a Dinucleotide Repeat Sequence Associated with a Common Fragile Site"
10:10 am - 10:15 am
Discussion
10:15 am - 10:25 am
MaryElizabeth Stein (Penn State College of Medicine, United States)
"The Significance of G-Quadruplex Variation to Mutagenesis: Sequence and Topology Differentially Impact Human DNA Polymerase Fidelity"
10:25 am - 10:30 am
Discussion
10:30 am - 11:00 am
Coffee Break
11:00 am - 11:20 am
Sarah Lambert (Institut Curie / CNRS, France)
"Understanding Replication Fork Processing and Restart at a Single Fork Resolution Level"
11:20 am - 11:30 am
Discussion
11:30 am - 11:40 am
Jacqueline Barlow (University of California, Davis, United States)
"Redundant Role of Senataxin and RNase H2 in Class Switch Recombination"
11:40 am - 11:45 am
Discussion
11:45 am - 11:55 am
Yuqing Feng (University of Toronto, Canada)
"FAM72A Antagonizes UNG2 to Promote Mutagenic Repair During Antibody Maturation"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:20 pm
Sergei Mirkin (Tufts University, United States)
"Genomic Weak Links: Mechanisms of Genome Instability Caused by Structure-Prone DNA Repeats"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 5:30 pm
Poster Session
5:30 pm - 7:30 pm
RNA-Dependent Mutagenesis
Discussion Leader: Thomas Kunkel (National Institute of Environmental Health Sciences, NIH, United States)
5:30 pm - 5:50 pm
Karlene Cimprich (Stanford University School of Medicine, United States)
"The Causes and Consequences of Replication Stress"
5:50 pm - 6:00 pm
Discussion
6:00 pm - 6:20 pm
Andres Aguilera (University of Seville, Spain)
"New Roles for Chromatin Remodelers in Co-Transcriptional R Loops and Associated Genome Instability"
6:20 pm - 6:30 pm
Discussion
6:30 pm - 6:40 pm
Oyku Sensoy (Vanderbilt University, United States)
"Investigating the Mechanisms Behind R-Loop Induced Lethality Upon Replication-Transcription Conflicts"
6:40 pm - 6:45 pm
Discussion
6:45 pm - 6:55 pm
Krystian Lazowski (Polish Academy of Sciences, Institute of Biochemistry and Biophysics, Poland)
"Division of Labour Between RNase HII and RNase HI During Ribonucleotide Excision Repair on the Leading and Lagging DNA Strands in E.coli"
6:55 pm - 7:00 pm
Discussion
7:10 pm - 7:30 pm
Hannah Klein (New York Grossman University School of Medicine, United States)
"Genome Damage and Mutations Arising From Persistent Ribonucleotides in DNA"
7:30 pm - 7:40 pm
Discussion
8:00 pm - 9:00 pm
Dinner
Thursday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Business Meeting
Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair
9:00 am - 12:30 pm
Environmental and Endogenous Mutagenesis
Discussion Leaders: Marcel Tijsterman (Leiden University Medical Center, The Netherlands)
9:00 am - 9:20 am
Shana Sturla (ETH Zurich, Switzerland)
"Tracking the Chemical Basis of Mutagenesis: From Missing Hydrogen Bonds in Bases to Genome-Wide Signatures"
9:20 am - 9:30 am
Discussion
9:30 am - 9:50 am
Juan Carvajal Garcia (Vanderbilt University, United States)
"Oxidative Damage Makes Nucleotide Excision Repair Mutagenic"
9:50 am - 10:00 am
Discussion
10:00 am - 10:10 am
Niketa Bhawsinghka (National Institute of Environmental Health Sciences, United States)
"Duplex Sequencing for Determining Bacterial Mutation"
10:10 am - 10:15 am
Discussion
10:15 am - 10:25 am
Kaitlynne Bohm (Washington State University, United States)
"Genome-Wide Maps of Rare UV Photoproducts Reveal Distinct Patterns of DNA Damage Formation in Chromatin"
10:25 am - 10:30 am
Discussion
10:30 am - 11:00 am
Coffee Break
11:00 am - 11:20 am
K.J. Patel (MRC Weatherall Institute of Molecular Medicine, United Kingdom)
"Aldehyde-Induced Mutagenesis"
11:20 am - 11:30 am
Discussion
11:30 am - 11:40 am
Sriram Vijayraghavan (MUSC, United States)
"Analyzing the Mutation Spectrum and Signature of Acetaldehyde in Yeast and Cancers"
11:40 am - 11:45 am
Discussion
11:45 am - 11:55 am
Nicola Zilio (Institute of Molecular Biology (IMB), Germany)
"Genome-Wide Mapping of DNA Damage by GLOE-Seq"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:20 pm
Bjoern Schwer (University of California, San Francisco, United States)
"DNA Double-Strand Break Repair and Neural Function"
12:20 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Evolvability and Adaptation
Discussion Leader: Houra Merrikh (Vanderbilt University, United States)
7:30 pm - 7:50 pm
Ivan Matic (Université Paris Cité, France)
"Heterogeneity of Spontaneous Mutation Rates Followed in Single Living Bacterial Cells"
7:50 pm - 8:00 pm
Discussion
8:00 pm - 8:20 pm
Melissa Gymrek (UNIVERSITY OF CALIFORNIA, SAN DIEGO, United States)
"The Mutational Landscape of Tandem Repeat Variation Across Human Populations"
8:20 pm - 8:30 pm
Discussion
8:30 pm - 8:40 pm
Sophia Tintori (New York University, United States)
"Nematode Genomes Reveal a Shift in Mutation Spectrum in the Chernobyl Exclusion Zone"
8:40 pm - 8:45 pm
Discussion
8:45 pm - 8:55 pm
Yuko Nakajima (Brandeis University, United States)
"Spontaneous Gene Conversion and Cas9-Induced Plasmid Loss in Cultured Borrelia"
8:55 pm - 9:00 pm
Discussion
9:00 pm - 9:20 pm
Daniel Jarosz (Stanford University, United States)
"High Frequency Drug Tolerance and Resistance: A Stress-Induced Interplay of Epigenetic and Genetic Mechanisms"
9:20 pm - 9:30 pm
Discussion
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure