Session 1: 7:30 PM - 9:20 PM, Sunday Evening, June 28. |
"Fixing Nitrogen-Overviews of the Genetic-Biochemical-Chemical Systems"
7:30-7:40 | Chair: Lance Seefeldt, Utah State University |
7:40-8:20 | Hans-Martin Fischer - Zurich "Genetic Overview" |
8:20-8:30 | Discussion |
8:30-9:10 | James B. Howard - Univ. of Minnesota "Overview of Chemistry and Biochemistry" |
9:10-9:20 | Discussion |
Session 2: 9:00 AM - 12:30 PM, Monday Morning, June 29. |
"Mechanisms of Gene Regulation"
9:00-9:20 | Chair: Christina Kennedy, University of Arizona "Introductory remarks" |
9:20-9:50 | Kenton Rodgers-North Dakota SU "Spectroscopic studies of the heme-based oxygen sensor, FixL" |
9:50-10:00 | Discussion |
10:00-10:30 | Coffee Break and Group Photo |
10:30-10:45 | Eric Soupenne-U.C. Berkeley "Ammonia acquisition in enteric bacteria: physiological role of AmtB" |
10:45-10:50 | Discussion |
10:50-11:20 | Stefan Nordlund-Stockholm U "Transcription of the glnBA operon and the role of ATase in Rhodospirillum rubrum" |
11:20-11:30 | Discussion |
11:30-12:00 | Martin Drummond-John Innes Centre "Multiple signals integrated by AnfA and VnfA in transcriptional control of the alternative nitrogenases" |
12:00-12:10 | Discussion |
12:10-12:25 | Emanuel Maltempi de Souza-UFPR Brazil "Control of the activity of NifA in Herbaspirillum seropedicae" |
12:25-12:30 | Discussion |
12:30 | Lunch |
Session 3: 4:00 PM - 5:30 PM, Monday Afternoon, June 29 |
"Poster Session-Regulation"
Chair-Ruth Schmitz, Gottingen-Germany
- Speakers selected from posters
Session 4: 7:30 PM - 10:00 PM, Monday Evening, June 29. |
"Related Systems"
7:30-7:40 | Chair: Lance Seefeldt, Utah State University |
7:40-8:20 | Robert Hausinger-Michigan State University "Urease" |
8:20-8:30 | Discussion |
8:30-9:10 | Ortwin Meyer-Germany "Superoxide-dependent nitrogenase" |
9:10-9:20 | Discussion |
9:20-9:50 | Huub Haaker-Netherlands "Nitrogenase mechanism" |
9:50-10:00 | Discussion |
Session 5: 9:00 AM - 12:30 PM, Tuesday Morning, June 30. |
"Biochemical Mechanism-Part I"
9:00-9:10 | Chair: Barbara Burgess, UC-Irvine |
9:10-9:35 | Douglas Rees-CalTech "Nitrogenase structures" |
9:35-9:45 | Discussion |
9:45-10:10 | Hayley Angove-UC Irvine "The roles of an all ferrous Fe protein" |
10:10-10:20 | Discussion |
10:20-10:50 | Coffee break |
10:50-11:15 | Britt Hedman-Stanford "XAS studies of nitrogenase" |
11:15-11:25 | Discussion |
11:25-11:50 | Brian Hales-LSU "Acetylene interactions with nitrogenase" |
11:50-12:00 | Discussion |
12:00-12:20 | Gary Watt-Brigham Young Univ. "ATP and the all ferrous Fe protein" |
12:20-12:30 | Discussion |
12:30 | Lunch |
Session 6: 7:30 PM - 10:00 PM, Tuesday Evening, June 30. |
"Biochemical Mechanism-Part II"
7:30-7:40 | Chair: Roger Thorneley, John Innes Centre |
7:40-8:05 | C.J. Pickett-John Innes Centre "FeMoco electrochemistry" |
8:05-8:15 | Discussion |
8:15-8:40 | S.J. George-John Innes Centre "Time resolved infra-red spectroscopy of functioning nitrogenase" |
8:40-8:50 | Discussion |
8:50-9:15 | David Lowe-John Innes Centre "ENDOR and ESEEM studies on nucleotide binding to nitrogenase" |
9:15-9:25 | Discussion |
Session 7: 9:00 AM - 12:30 PM, Wednesday Morning, July 1. |
"Metallocenter Assembly"
9:00-9:10 | Chair: Paul Ludden, University of Wisconsin |
9:10-9:30 | Gary Roberts-U. of Wisconsin "Maturation of the NifDK gene products-the role of gamma" |
9:30-9:40 | Discussion |
9:40-10:05 | C. Rutiman-Johnson-U. Wisconsin "The role of VnfX in the biosynthesis of FeV-co" |
10:05-10:15 | Discussion |
10:15-10:40 | Coffee break |
10:40-11:05 | Dorothea Kessler-Germany "Cysteine C-S-lyase in FeS cluster synthesis" |
11:05-11:15 | Discussion |
11:15-11:40 | Werner Klipp-Germany "Molybdenum metabolism in Rhodobacter capsulatus" |
11:40-11:50 | Discussion |
11:50-12:05 | P.J. Goodwin-VPI "NifNE properties" |
12:05-12:10 | Discussion |
12:10-12:25 | Richard Pau-John Innes Centre "Early steps in molybdenum metabolism" |
12:25-12:30 | Discussion |
12:30 | Lunch |
Session 8: 4:00 PM - 5:30 PM, Wednesday Afternoon, July 1. |
"Poster Session-Mechanism/Assembly"
Chair-John Peters, Utah State University
| W. Lanzilotta-Utah S.U. "Role of nitrogenase P-clusters" |
| N. Gavini-Bowling Green SU "A possible role for the NifM in biological nitrogen fixation" |
| -Additional speakers selected from posters |
Session 9: 7:30 PM - 10:20 PM, Wednesday Evening, July 1. |
"Chemistry of Nitrogen Fixation"
7:30-7:40 | Chair: Edward Stiefel, Exxon Research |
7:40-8:10 | Richard Holm-Harvard "Metallocenter models of nitrogenase" |
8:10-8:20 | Discussion |
8:20-8:50 | Dieter Sellmann-Germany "The relevance of chemical principles and structural copies for the N2 fixation problem" |
8:50-9:00 | Discussion |
9:00-9:30 | Richard Henderson-John Innes Centre "Why R-homocitrate is essential for nitrogen fixation: Kinetic studies on extracted FeMo-cofactor" |
9:30-9:40 | Discussion |
9:40-10:10 | Dimitri Coucouvanis-Univ. of Michigan "Synthesis and reactivity
of new Fe/Mo/S clusters of possible relevance to nitrogenase function" |
10:10-10:20 | Discussion |
Session 10: 9:00 AM - 12:30 PM, Thursday Morning, July 2. |
"Nitrogen Cycle"
9:00-9:10 | Chairs: Dan Arp, Oregon State Univ. and Walter Zumft, Germany |
9:10-9:40 | Dan Arp-Oregon S.U. "Ammonia monooxygenase from Nitrosomonas europaea" |
9:40-9:50 | Discussion |
9:50-10:20 | David Arciero-U. of Minnesota "Hydroxylamine oxidoreductase of Nitrosomonas" |
10:20-10:30 | Discussion |
10:30-10:50 | Coffee break |
10:50-11:20 | Walter Zumft-Germany "Denitrification reactions" |
11:20-11:30 | Discussion |
11:30-12:00 | Michael Barber-U. of South Florida "Nitrate reductase" |
12:00-12:10 | Discussion |
12:30 | Lunch |
Session 11: 8:00 PM - 9:30 PM, Thursday Evening, July 2. |
Round Table Discussion: "Challenges for the Next Two Years"
Discussion Leader: William Newton, VPI.
- Panelists:
- Roger Thorneley, John Innes Centre
- Barry Smith, John Innes Centre
- Cees Veeger, The Netherlands
- Christina Kennedy, University of Arizona
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