| Sunday Afternoon, July 2 |
| 2:00 PM - 6:00 PM | Registration and Check-in |
| Sunday Evening | Overview |
| 7:30-7:40 | Discussion Leader: Paul Ludden, University of Wisconsin |
| 7:40-8:30 | Barry Smith, John Innes Centre
"The Development of Chemical, Biological, and Genetic Approaches to the Study of Nitrogen Fixation Over the Past 30 Years" |
| 8:30-9:00 | Discussion |
| 9:00- | Reception |
| Monday Morning | Nitrogenase Mechanism: Chemical Aspects |
| 9:00-9:10 | Chair: Barbara Burgess, University of California-Irvine |
| 9:10-9:40 | Richard Holm, Harvard University
"Construction of bridged metal assemblies relevant to protein clusters" |
| 9:40-9:50 | Discussion |
| 9:50-10:20 | Patrick Frank, Stanford University
"Ligand binding constants and evidence for FeMoco self-association complexes in NMF solution" |
| 10:20-10:30 | Discussion |
| 10:30-11:00 | Coffee Break |
| 11:00-11:30 | Felix Tuczek, Christian Albrechts University, Kiel
"Understanding elementary steps of nitrogen fixation: theoretical and experimental results" |
| 11:30-11:40 | Discussion |
| 11:40-12:10 | Dimitri Coucouvanis, University of Michigan
"Is metal-metal bonding important in the function of nitrogenase?" |
| 12:10-12:20 | Discussion |
| 12:30-2:00 | Lunch |
| Monday Evening | Iron-molybdenum Cofactor Models and Reactivity |
| 7:30-7:40 | Chair: Robert Eady, John Innes Centre |
| 7:40-8:10 | Eckard Münck, Carnegie Mellon
"Recent Mössbauer studies of the MoFe and Fe proteins from Azotobacter vinelandii" |
| 8:10-8:20 | Discussion |
| 8:20-8:50 | Chris Pickett, John Innes Centre
"Electrochemically probing the interactions of small molecules with isolated FeMoco" |
| 8:50-9:00 | Discussion |
| 9:00-9:30 | Simon George, John Innes Centre
"Stopped-flow FTIR of the nitrogenase-CO complex" |
| 9:30-9:40 | Discussion |
| 9:40-10:10 | Brian Hoffman, Northwestern University
"ENDOR spectroscopy of nitrogenase reaction intermediates" |
| 10:10-10:20 | Discussion |
| Tuesday Morning | Nitrogenase Substrates and Ligands/Related Systems |
| 9:00-9:10 | Chair: Roger Thorneley, John Innes Centre |
| 9:10-9:40 | Dennis Dean, Virginia Tech
"Nitrogenase catalytic site" |
| 9:40-9:50 | Discussion |
| 9:50-10:20 | Jeannine Chan, Utah State University
"Substrate and inhibitor interactions with nitrogenase" |
| 10:20-10:30 | Discussion |
| 10:30-11:00 | Coffee Break and Group Photo |
| 11:00-11:30 | William Newton, Virginia Tech
"Insights into substrate reduction by Azotobacter vinelandii molybdenum-nitrogenase" |
| 11:30-11:40 | Discussion |
| 11:40-12:10 | Brian Hales, Louisiana State University
"Turnover states of Mo-nitrogenase with substrates or inhibitors bound" |
| 12:10-12:20 | Discussion |
| 12:30-2:00 | Lunch |
| Tuesday Evening | Fe Protein and Nitrogenase Complex Structures |
| 7:30-7:40 | Chair: Lance Seefeldt, Utah State University |
| 7:40-8:20 | John Peters, Utah State University
"Nucleotide interactions in the nitrogenase Fe protein" |
| 8:20-8:30 | Discussion |
| 8:30-9:10 | Robert Eady, John Innes Centre
"Properties and reactivity of stable nitrogenase complexes" |
| 9:10-9:20 | Discussion |
| 9:20-9:50 | Gary Watt, Brigham Young
"Comparison of characteristic nitrogenase reactions using the [Fe4S4]1+ and the [Fe4S4]2+/[Fe4S4]1+ redox couples" |
| 9:50-10:00 | Discussion |
| Wednesday Morning | Biosynthesis of Iron-Molybdenum Cofactor/Related System |
| 9:00-9:10 | Chair: Paul Ludden, University of Wisconsin |
| 9:10-9:40 | Barbara Burgess, University of California-Irvine
"Azotobacter vinelandii nitrogenase: identification of an Fe-protein residue (E146) that is specifically involved in FeMo cofactor insertion" |
| 9:40-9:50 | Discussion |
| 9:50-10:20 | Nara Gavini, Bowling Green State University
"Role of NifM in nitrogenase maturation" |
| 10:20-10:30 | Discussion |
| 10:30-11:00 | Coffee Break |
| 11:00-11:30 | Priya Rangaraj, University of Wisconsin
"Biosynthesis of iron-molybdenum & iron-vanadium cofactors of nitrogenase" |
| 11:30-11:40 | Discussion |
| 11:40-12:10 | Christina Kennedy, University of Arizona
"Evidence for an alternative V-containing nitrate reductase in Azotobacter vinelandii" |
| 12:10-12:20 | Discussion |
| 12:30-2:00 | Lunch |
| Wednesday Evening | Biosynthesis of Iron-Sulfur Clusters in Enzymes Other Than Nitrogenase |
| 7:30-7:40 | Chair: Dennis Dean, Virginia Tech |
| 7:40-8:20 | Yuichi Fujita, Osaka University
"Nitrogenase-like features of a (bacterio)chlorophyll biosynthesis enzyme" |
| 8:20-8:30 | Discussion |
| 8:30-9:10 | Michael K. Johnson, University of Georgia
"Iron-sulfur cluster biosynthesis" |
| 9:10-9:20 | Discussion |
| 9:20-9:50 | Roland Lill, Universität Marburg
"The essential role of mitochondria in the biogenesis of cellular Fe/S proteins" |
| 9:50-10:00 | Discussion |
| Thursday Morning | Regulation of Nitrogenase |
| 9:00-9:10 | Chair: Claudine Elmerich, Institut Pasteur |
| 9:10-9:40 | Ray Dixon, John Innes Centre
Signal transduction to the Azotobacter vinelandii NIFL-NIFA regulatory system in response to fixed nitrogen is influenced directly by interaction with
2-oxoglutarate and the PII regulatory protein" |
| 9:40-9:50 | Discussion |
| 9:50-10:20 | James Golden, Texas A & M
"PatS peptide influences heterocyst developmental pattern in Anabaena" |
| 10:20-10:30 | Discussion |
| 10:30-11:00 | Coffee Break |
| 11:00-11:30 | Stefan Nordlund, Stockholm University
"Regulation of nitrogenase in Rhodospirillum rubrum: on the role of the chromatophore membranes" |
| 11:30-11:40 | Discussion |
| 11:40-12:10 | Claudine Elmerich, Institut Pasteur
"Regulation of the synthesis and activity of the nif specific transcription activator NifA" |
| 12:10-12:20 | Discussion |
| 12:30-2:00 | Lunch |
| Thursday Evening | Banquet |
| Dennis Dean
"Reflections on Nitrogenase" |
