| SUNDAY |
| 2:00 pm - 9:00 pm | Arrival and check-in |
| 6:00 pm | Dinner |
| 7:30 pm - 7:40 pm | Welcome & Introductory Remarks
Brian Hales (Louisiana State University), Chair |
| 7:40 pm - 9:20 pm | REACTIVITY OF NITROGENASE |
| Discussion Leader: Larry Davis (Kansas State University) |
| 7:40 pm - 8:30 pm | Michael Dilworth (Murdoch University)
"Nitrogenase Substrate Reduction - Historical and Hysterical" |
| 8:30 pm - 9:20 pm | Robert Eady (John Innes Centre) &
Markus Ribbe (University of California, Irvine)
"Tributes to Past Colleagues" |
| 9:20 pm | Chair's Reception |
| MONDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | SUBSTRATE/COFACTOR INTERACTIONS |
| Discussion Leader: Robert Eady (John Innes Centre) |
| 9:00 am - 9:40 am | Suzy Mayer (Virginia Tech)
"Substrate Interaction with FeMo-Cofactor" |
| 9:40 am - 10:20 am | Lance Seefeldt (Utah State University)
"Protein Control of Substrate Access to FeMo-Cofactor" |
| 10:20 am | Coffee Break and Photo Session |
| 11:10 am - 11:50 am | Hong In Lee (Kyungpook National University)
"ENDOR Studies of Trapped Substrates" |
| 11:50 am - 12:30 pm | William Newton (Virginia Tech)
"Transient Species Detected by EPR Spectroscopy during Nitrogenase Turnover" |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | MODEL SYSTEMS |
| Discussion Leader: Paul Ludden (University of
Wisconsin) |
| 7:30 pm - 8:10 pm | Dimitri Coucouvanis (University of Michigan)
"An Examination of the Structure of the Nitrogenase Cofactor and Arguments Suggesting a Thermodynamic Metastable Species" |
| 8:10 pm - 8:50 pm | Bruce Averill (University of Toledo)
"Modeling the Structure of the NifEN Complex, a Putative Scaffold for the Biosynthesis of FeMo-co" |
| 8:50 pm - 9:30 pm | Richard Henderson (University of Newcastle)
"Rates and Mechanism of Binding Substrates to Fe-S Clusters" |
| TUESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | FE-S CLUSTER FUNCTION AND ASSEMBLY |
| Discussion Leader: Dennis Dean (Virginia Tech) |
| 9:00 am - 9:40 am | Catherine Drennen (Massachusetts Institute of Technology)
"The Ever Surprising Ni-Fe-S Clusters of Carbon Monoxide Dehydrogenase" |
| 9:40 am - 10:20 am | Vincent Huynh (Emory University)
"Fe-S Cluster Assembly in nif-Specific Scaffold Proteins: A Mössbauer Investigation" |
| 10:20 am | Coffee Break |
| 10:45 am - 11:25 am | James Cowan (Ohio State University)
"Molecular Pathways for Fe-S Assembly" |
| 11:25 am - 12:05 pm | Patricia Kiley (University of Wisconsin)
"Regulation of Fe-S Cluster Assembly" |
| 12:05 pm - 12:30 pm | Patrick Holland (University of Rochester)
"Synthetic Complexes of Three-Coordinate Iron with
Ligands of Relevance to FeMoco Function" |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | NITROGENASE Cofactor
|
| Discussion Leader: Lance Seefeldt (Utah State University) |
| 7:30 pm - 8:10 pm | Paul Ludden (University of Wisconsin)
"NifX, VnfX, NifY and NifH as Binding Proteins for
FeMo-co or its Precursors" |
| 8:10 pm - 8:50 pm | Stephan Cramer (University of California, Davis)
"(All Sorts of) X-Ray Spectroscopy of Nitrogenase and
Hydrogenase" |
| 8:50 pm - 9:30 pm | Kresimir Rupnik (Louisiana State University)
"Spectroscopic Studies of Turnover States" |
| WEDNESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | HYDROGENASE |
| Discussion Leader: Michael Johnson (University of Georgia) |
| 9:00 am - 9:40 am | Richard Cammack (King's College London)
"ENDOR Studies of Structure and Mechanism of Hydrogenases" |
| 9:40 am - 10:20 am | Juan Fontecilla-Champ (Institut de Biologie Structurale Jean-Pierre Ebel)
"Structural Bases of Hydrogen Biocatalysis" |
| 10:20 am | Coffee Break |
| 10:45 am - 11:25 | Irene Georgakaki (Texas A&M)
"Fundamental Properties of Diiron Complexes Related to Fe-Only Hydrogenase" |
| 11:25 am - 12:05 | Simon George (John Innes Centre)
"Stopped-Flow FTIR of Hydrogenase, Nitrogenase and Model Systems" |
| 12:05 pm - 12:30 pm | General Discussion of Posters |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | THEORETICAL MODELS |
| Discussion Leader: Kresimir Rupnik (Louisiana State
University) |
| 7:30 pm - 8:10 pm | Marcus Durrant (John Innes Centre)
"A Self-Consistent Theoretical Model for Nitrogenase Action on Dinitrogen, Protons, Dideuterium and Acetylene" |
| 8:10 pm - 8:50 pm | David Beratan (Duke University)
"Theory of ATP Driven Electron Transfer in Nitrogenase" |
| 8:50 pm - 9:30 pm | Lou Noodlemen (Scripps Research Institute)
"Insights into Properties and Energetics for the Nitrogenase Reaction Cycle from Density Function and Electrostatic Calculations" |
| THURSDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | STRUCTURE AND THE FE-PROTEIN |
| Discussion Leader: Simon George (John Innes Centre) |
| 9:00 am - 9:40 am | Markus Ribbe (University of California, Irvine)
"Structural and Biochemical Characterization of FeMoco-deficient MoFe proteins" |
| 9:40 am - 10:20 am | John Peters (Utah State University)
"Structural Characterization of the A. vinelandii Fe Protein L127 Deletion Variant" |
| 10:20 am | Coffee Break |
| 10:45 am - 11:25 am | Gary Watt (Brigham Young University)
"Reactivity of the Fe Protein in all Three of its Redox States, 2+, 1+ and 0" |
| 11:25 am - 12:05 pm | Larry Davis (Kansas State University)
"Nitrogenase Complex Formation by Fe Protein Mutants" |
| 12:05 pm - 12:30 pm | Nara Gavini (Bowling Green State University)
"The NifM Independent Fe-protein of Nitrogenase." |
| 12:30 pm | Lunch |
| 1:30 pm - 6:00 pm | Free Time |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | REACTIVITY OF NITROGENASE |
| Discussion Leader: Brian Hales (Louisiana State University) |
| 7:30 pm - 8:30 pm | Robert Eady (John Innes Centre)
"Kinetic Studies Probing the Half-Site Reactivity of Nitrogenase" |
| 8:30 pm - 9:00 pm | Participants
General Discussion |
| FRIDAY |
| 7:30 am - 8:30 pm | Breakfast |
| 9:00 am | Departure |
