| SUNDAY |
| 2:00 pm - 9:00 pm | Arrival and check-in |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | Breaking Even with Nitrogenase |
| 7:30 pm - 7:45 pm | Discussion Leader: L. Davis (K-State)
"Welcome and Introductory remarks" |
| 7:45 pm - 8:30 pm | P. W. Ludden (Berkeley)
"Contributions of R.H. Burris, in recognition of his 90th birthday" |
| 8:30 pm - 9:30 pm | D. Rees (Caltech)
"Breaking N2 to fix it: mechanistic challenges of nitrogenase"
[Alexander Cruickshank lecture in Chemistry] |
| 9:30 pm | Chair's Reception |
| MONDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | Chemical Models and Reactions |
| 9:00 am - 9:15 am | Discussion Leader: C. Pickett (John Innes)
"General information for atendees and introductory remarks" |
| 9:15 am - 10:00 am | R.H. Holm (Harvard)
"Building Better Clusters" |
| 10:00 am - 10:30 am | Coffee Break |
| 10:30 am - 11:00 am | L.-S. Wang (Pacific Northwest Nat'l Lab)
"Probing the intrinsic electronic structure of the Fe4S4 cubanes in the gas
phase" |
| 11:00 am - 11:30 am | P. Holland (U. of Rochester)
"Synthetic chemistry of low-coordinate iron: hydrides that break strong bonds" |
| 11:30 am - 12:00 pm | P. Chirik (Cornell)
"Extending the range of metals able to activate dinitrogen: the case of zirconium" |
| 12:00 pm - 12:30 pm | D. Coucouvanis (U. of Michigan)
"Experimental and theoretical explorations of structure-function relationships in nitrogenase" |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 5:00 pm | Poster Session (Even # presenters) |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | Nitrogenase Mechanism I |
| Discussion Leader: W.E. Newton (Virginia Tech)
"Introductory comments" |
| 7:40 pm - 8:15 pm | S.P. Cramer (UC-Davis)
From kilovolts to millivolts- characterization of nitrogenase and FeS model compounds using synchrotron vibrational M ssbauer spectroscopy" |
| 8:15 pm - 8:50 pm | B. Hoffman (Northwestern)
"Characterization of nitrogenase turnover intermediates by ENDOR spectroscopy" |
| 8:50 pm - 9:25 pm | B. Hales (Louisiana State U.)
"Role of the nitrogenase P cluster in electron transfer and substrate reduction" |
| 9:25 pm - 9:30 pm | Discussion |
| TUESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | Computational Approaches to the Chemistry of Clusters |
| Discussion Leader: T. Ichiye (Georgetown) |
| 9:00 am - 9:45 am | B. Hinnemann (Technical University of Denmark)
"Density functional studies of the FeMo cofactor structure and reactivity" |
| 9:45 am - 10:30 am | J. Kästner (Clausthal Univ. Tech.)
"Active role of the FeMoco cage: a comprehensive model of the mechanism from first principles calculations |
| 10:30 am - 11:00 am | Coffee Break |
| 11:00 am - 11:45 am | E.L. Bominaar (Carnegie-Mellon U.)
"M ssbauer/DFT studies of FeMoco" |
| 11:45 am - 12:30 pm | L. Noodleman (Scripps)
"Recent theoretical studies of the electronic states and reactions of the FeMo cofactor center in nitrogenase" |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 5:00 pm | Poster Session (Odd # presenters) |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | Nitrogenase Mechanism II |
| Discussion Leader: B. Hales (Louisiana State. U.)
"Introductory comments" |
| 7:40 pm - 8:15 pm | A. Kohen (U. of Iowa)
"Nitrogenase catalyzed N2 reduction: 15N isotope effects studies" |
| 8:15 pm - 8:50 pm | K. Fisher (John Innes)
"Kinetic and spectroscopic studies on mutants of the MoFe protein of nitrogenase" |
| 8:50 pm - 9:25 pm | R. Igarashi (Utah State)
"Localization of substrate reduction intermediates on the FeMocofactor within the a-70 Ala MoFe protein" |
| 9:25 pm - 9:30 pm | General Discussion |
| WEDNESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | Nitrogenase Fe Protein and Related Enzymes |
| Discussion leader: P. Ludden |
| 9:00 am - 9:45 am | M. Ribbe (UC-Irvine)
"P-cluster assembly of nitrogenase MoFe protein" |
| 9:45 am - 10:30 am | J. Peters (Montana State U.)
"Role of MgATP in nitrogenase catalysis" |
| 10:30 am - 11:00 am | Coffee Break |
| 11:00 am - 11:45 am | J. Jarrett (U. of Pennsylvania)
"Enzyme-catalyzed FeS cluster destruction: the unique structure and
mechanism and the AdoMet radical enzyme biotin synthase" |
| 11:45 am - 12:30 pm | W. B ckel (U. of Marburg)
"Electron transfer in the dehydration of 2-hydroxyacyl CoA and nitrogen
fixation" |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | Hydrogenase |
| Discussion Leader: B. Hoffman (Northwestern)
"Introductory comments" |
| 7:40 pm - 8:15 pm | S.J. George (Lawrence Berkeley Nat'l Lab)
"Activation and catalysis of Ni-Fe hydrogenase and stopped flow infrared
spectroscopy" |
| 8:15 pm - 8:50 pm | J. Fontecilla (CEA/CNRS-Grenoble)
"Procaryotic Fe hydrogenases and their eucaryotic homologs" |
| 8:50 pm - 9:25 pm | C. Pickett (John Innes)
"Synthesis of the H-cluster of Fe-only hydrogenase" |
| 9:25 pm - 9:30 pm | General discussion |
| THURSDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | FeS cluster biosynthesis |
| Discussion leader: D. Dean (Virginia Tech) |
| 9:00 am - 9:45 am | D. Johnson (Virginia Tech)
"Controlled expression of FeS biosynthetic proteins" |
| 9:45 am - 10:30 am | J.A. Cowan (Ohio State U.)
"Molecular mechanisms of iron-sulfur cluster assembly, transport and delivery" |
| 10:30 am - 11:00 am | Coffee Break |
| 11:00 am - 11:45 am | L. Rubio (UC - Berkeley)
"The iron-molybdenum cofactor insertase: characterization of the NafY protein from Azotobacter vinelandii" |
| 11:45 am - 12:30 pm | M. Newcomer (Louisiana State U.)
"Structural studies on IscA- a cluster carrier" |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | Evolution and the way ahead |
| Discussion Leader: J. Peters (Montana State U.)
"Introductory comments" |
| 7:40 pm - 8:00 pm | N. Gavini (Bowling Green U.)
"ChlL complementation of nifH minus strains of Azotobacter" |
| 8:00 pm - 8:35 pm | R. Lill (U. of Marburg)
"Iron sulfur protein maturation in eucaryotes" |
| 8:35 pm - 9:20 pm | R. N.F. Thorneley (John Innes)
"The future of nitrogenase in light of the past" |
| 9:20 pm - 9:30 pm | General Discussion |
| FRIDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am | Departure |
