Sunday |
4:00 pm - 8:00 pm | Arrival and Check-in |
6:00 pm | Dinner |
7:30 pm - 7:40 pm | Welcome / Introductory Comments by GRC Site Staff |
7:40 pm - 9:30 pm | Keynote Session: Light-Gated
Ion Channels: Function and Application |
| The advent of
optogenetics has come primarily from research on light-activated ion channels.
Channel rhodopsins (ChRs) have been studied widely at the biophysical and more
recently at the structural level. Peter Hegemann will lead this opening session and pioneered the earliest studies
of ChR, first demonstrating that it functions as a light-activated cation channel.
ChR was later exploited to control neural activity by light. This session is
supplemented with two groundbreaking advances in generating alternative tools
for controlling neural activity with possible applications in medicine. |
| Discussion Leader: Peter Hegemann (Humboldt-Universitaet zu Berlin, Germany) |
7:40 pm - 7:50 pm | Introduction by Discussion Leader |
7:50 pm - 8:10 pm | Ehud Isacoff (University of California, Berkeley, USA) "Engineering Photoreception into Neurotransmitter Receptors" |
8:10 pm - 8:20 pm | Discussion |
8:20 pm - 8:40 pm | Anna Moroni (University of Milan, Italy) "Engineering
and Characterization of a Light-Gated Potassium Channel" |
8:40 pm - 8:50 pm | Discussion |
8:50 pm - 9:20 pm | Peter Hegemann (Humboldt-Universitaet zu Berlin, Germany) "Enzyme-Rhodopsins" |
9:20 pm - 9:30 pm | Discussion |
Monday |
7:30 am - 8:30 am | Breakfast |
8:30 am | Group Photo |
9:00 am - 12:30 pm | Opsin Structure and Diversity |
| Visual
and sensory rhodopsins have been studied extensively from the biophysical,
structural, physiological and photobiological perspectives. This session integrates these aspects of this diverse photoreceptor class from microbial
to mammalian systems.
The session will also extend this focus to novel tools generated from opsin
research to create artificial reporters of membrane excitability. |
| Discussion Leader: Hideki Kandori (Nagoya Institute of Technology, Japan) |
9:00 am - 9:10 am | Introduction by Discussion Leader |
9:10 am - 9:30 am | John Spudich (University of Texas Medical School at Houston, USA) "Diversity of Sensory Rhodopsin Mechanisms" |
9:30 am - 9:40 am | Discussion |
9:40 am - 10:00 am | Oliver Ernst (University of Toronto, Canada) "Structure and Dynamics of Rhodopsin" |
10:00 am - 10:10 am | Discussion |
10:10 am - 10:25 am | Hoi Ling Luk (Bowling Green State University, USA) "Molecular Basis for Chromophore Selection in Animal Rhodopsins" |
10:25 am - 10:30 am | Discussion |
10:30 am - 11:00 am | Coffee Break |
11:00 am - 11:20 am | Robert Lucas (University of Manchester, United Kingdom) "Signaling Functions and Optogenetic Applications
of Animal Opsins" |
11:20 am - 11:30 am | Discussion |
11:30 am - 11:50 am | Adam Cohen (Harvard University, USA) "Photocycle Control of Microbial Rhodopsins for Voltage Imaging" |
11:50 am - 12:00 pm | Discussion |
12:00 pm - 12:20 pm | Hideki Kandori (Nagoya Institute of Technology, Japan) "Mechanism
of Active Ion-Transport by Light" |
12:20 pm - 12:30 pm | Discussion |
12:30 pm | Lunch |
1:30 pm - 4:00 pm | Free Time |
3:00 pm - 4:00 pm | Power Hour |
| The GRC Power Hour is an optional informal gathering open to all meeting participants. It is designed to help address the challenges women face in science and support the professional growth of women in our communities by providing an open forum for discussion and mentoring. |
| Organizers: Silvia Braslavsky (Max Planck Institute for Chemical Energy Conversion, Germany) and Aba Losi (University of Parma, Italy) |
4:00 pm - 6:00 pm | Poster Session |
6:00 pm | Dinner |
7:30 pm - 9:30 pm | Responses
to UV Irradiation |
| UV irradiation
can be damaging to mammals, but in plants and algae this light quality can be
utilized, through unique mechanisms, to control developmental processes in
addition to stimulating photoprotection responses. This session will integrate diverse researchers in the field to discuss the different
mechanisms that govern UV perception and signaling responses from biophysical,
structural and molecular levels. |
| Discussion Leader: Roman Ulm (University of Geneva, Switzerland) |
7:30 pm - 7:40 pm | Introduction by Discussion Leader |
7:40 pm - 8:00 pm | Takahiro Yamashita (Kyoto University, Japan) "Molecular Properties of a Universal UV-Light Sensitive Opsin in Vertebrates" |
8:00 pm - 8:10 pm | Discussion |
8:10 pm - 8:25 pm | Alfred Batschauer (Philipps-Universität Marburg, Germany) "Function and Evolution of DASH-Type Cryptochromes in Fungi" |
8:25 pm - 8:30 pm | Discussion |
8:30 pm - 8:50 pm | Xiaojing Yang (University of Illinois at Chicago, USA) "Molecular Mechanism of UV-B Perception in UVR8: A Dynamic Crystallographic Study" |
8:50 pm - 9:00 pm | Discussion |
9:00 pm - 9:20 pm | Roman Ulm (University of Geneva, Switzerland) "UV-B Photoreceptor Signaling in Plants" |
9:20 pm - 9:30 pm | Discussion |
Tuesday |
7:30 am - 8:30 am | Breakfast |
9:00 am - 12:30 pm | LOV to BLUF: Flavoprotein Photosensors |
| Flavin-based
blue-light receptors are widespread throughout nature, being highly prevalent
in bacteria, fungi and plants. Two versatile flavin-binding photosensory motifs
have been identified and this session focuses on comparing these photoreceptor
proteins from plants and microbes, spanning the biophysical to the
physiological. The session will also incorporate artificially engineered photosensors
that place biological processes under spatio-temporal control. |
| Discussion Leader: Kevin Gardner (CUNY Advanced Science Research Center, USA) |
9:00 am - 9:10 am | Introduction by Discussion Leader |
9:10 am - 9:30 am | Masamitsu Wada (Tokyo Metropolitan University, Japan) "How to Raise the Motive Force for Chloroplast Relocation Movement" |
9:30 am - 9:40 am | Discussion |
9:40 am - 10:00 am | Aba Losi (University of Parma, Italy) "A Home-Made
FRET Couple Composed of Flavin- and Bilin-Binding Photoreceptors" |
10:00 am - 10:10 am | Discussion |
10:10 am - 10:25 am | John Kennis (VU University Amsterdam, The Netherlands) "Photoactivation Mechanisms of Blue-Light Receptors
Assessed Through Time-Resolved Vibrational Spectroscopy" |
10:25 am - 10:30 am | Discussion |
10:30 am - 11:00 am | Coffee Break |
11:00 am - 11:10 am | Brian Zoltowski (Southern Methodist University, USA) "Integrating Stress Response Pathways with a Little LOV" |
11:10 am - 11:15 am | Discussion |
11:15 am - 11:25 am | Oskar Berntsson (University of Gothenburg, Sweden) "Resolving Signal Transduction in Photosensory Histidine Kinases by X-Ray Solution Scattering" |
11:25 am - 11:30 am | Discussion |
11:30 am - 11:50 am | Lukas Kapitein (Utrecht University, The Netherlands) "Using Light to Dissect and Direct Intracellular Transport" |
11:50 am - 12:00 pm | Discussion |
12:00 pm - 12:20 pm | Kevin Gardner (CUNY Advanced Science Research Center, USA) "Better Tools Through Synergy: Necessity of Integrating Basic and Applied Science for Optogenetics" |
12:20 pm - 12:30 pm | Discussion |
12:30 pm | Lunch |
1:30 pm - 4:00 pm | Free Time |
4:00 pm - 6:00 pm | Poster Session |
6:00 pm | Dinner |
7:30 pm - 9:30 pm | Cryptochrome Structure and Function |
| Cryptochrome was first identified in plants, but is also
integral to circadian entrainment in mammals and insects. The primary
photochemical events associated with these photoreceptors are still not fully
resolved nor is their responsiveness to magnetic fields. This session will
focus on structural and functional aspects of the cryptochrome class of UV/blue
light receptor in different organisms. |
| Discussion Leader: Brian Crane (Cornell University, USA) |
7:30 pm - 7:40 pm | Introduction by Discussion Leader |
7:40 pm - 8:00 pm | Margaret Ahmad (Pierre and Marie Curie University, France) "Cryptochrome Photoreceptor Mechanism of Activation" |
8:00 pm - 8:10 pm | Discussion |
8:10 pm - 8:30 pm | Todd Holmes (University of California, Irvine School of Medicine, USA) "Cryptochrome-Mediated Spectral Responses" |
8:30 pm - 8:40 pm | Discussion |
8:40 pm - 8:50 pm | Sophie Franz (Philipps University Marburg, Germany) "Insights into the Structure and Functionality of CraCRY" |
8:50 pm - 8:55 pm | Discussion |
8:55 pm - 9:05 pm | Alex Jones (University of Manchester, United Kingdom) "Cryptochrome-Dependent Magnetic Field Effects on Neuronal Activity" |
9:05 pm - 9:10 pm | Discussion |
9:10 pm - 9:25 pm | Brian Crane (Cornell University, USA) "Activation Mechanisms of Drosophila Cryptochrome" |
9:25 pm - 9:30 pm | Discussion |
Wednesday |
7:30 am - 8:30 am | Breakfast |
9:00 am - 12:30 pm | Bilin-Based
Photosensory Systems |
| Bilins are
heme-derived natural products widespread in nature, which intrinsically absorb
red light. They are tuned to harvest a broad spectral range of visible light
and to transfer excitation energy to photosynthetic reaction centers when
acting as chromophores of phycobiliprotein antennae. As chromophores of
phytochromes and cyanobacteriochromes, bilins function as photoactive switches
in protein environments that tune bilin spectra, facilitate reversible bilin
photoisomerization, and modulate stability of the isomerized photoproduct. This
session explores the spectral diversity, evolution, structure and synthetic
biological applications of representative members of this diverse superfamily
found in plants, algae, eubacteria, fungi and heterokonts. |
| Discussion Leader: J. Clark Lagarias (University of California, Davis, USA) |
9:00 am - 9:10 am | Introduction by Discussion Leader |
9:10 am - 9:30 am | Sarah Mathews (Commonwealth Scientific and Industrial Research Organisation, Australia) "Molecular Evolution of Phytochrome Signaling Pathways in Land Plants" |
9:30 am - 9:40 am | Discussion |
9:40 am - 10:00 am | Richard Vierstra (Washington University in St. Louis, USA) "Atomic Perspectives on Phytochrome Photoactivation" |
10:00 am - 10:10 am | Discussion |
10:10 am - 10:25 am | Emina Stojkovic (Northeastern Illinois University, USA) "Structure and Function of Phytochromes in Stigmatella
aurantiaca: Light-Controlled Morphogenesis of Myxobacteria" |
10:25 am - 10:30 am | Discussion |
10:30 am - 11:00 am | Coffee Break |
11:00 am - 11:10 am | Heikki Takala (University of Helsinki, Finland) "Light-Induced Changes and Dimerization of Deinococcus radiodurans Phytochrome" |
11:10 am - 11:15 am | Discussion |
11:15 am - 11:25 am | Heewhan Shin (University of Illinois at Chicago, USA) "Crystal Structure and Signaling Mechanism of Red/Green Absorbing Cyanobacteriochrome" |
11:25 am - 11:30 am | Discussion |
11:30 am - 11:50 am | Jeff Tabor (Rice University, USA) "Characterising and Controlling Gene Expression
Dynamics via Phytochrome-Family Two Component Systems" |
11:50 am - 12:00 pm | Discussion |
12:00 pm - 12:20 pm | J. Clark Lagarias (University of California, Davis, USA) "Illuminating Phytochrome Heterogeneity" |
12:20 pm - 12:30 pm | Discussion |
12:30 pm | Lunch |
1:30 pm - 4:00 pm | Free Time |
4:00 pm - 6:00 pm | Poster Session |
6:00 pm | Dinner |
7:00 pm - 7:30 pm | Business Meeting |
| Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair |
7:30 pm - 9:30 pm | Microbial Light Sensing, Function and Applications |
| Photoreceptor
systems in microbes include sensors of all wavelength ranges and provide facile
systems for genetic manipulation and rapid phenotypic characterization. Yet
other than for sensory opsins and regulation of photosynthetic capacity, the
overall functions of these proteins in bacteria remain poorly understood. This
session offers key examples of such systems and will
discuss how diverse modes of phototransduction regulate photosynthetic function, as well as their role in controlling stress responses
and pathogenicity in non-photosynthetic bacteria. This session will also highlight the diversity of photoreceptor processes in nature with, as yet untapped, potential as optogenetic switches or for fluorescent imaging in the near infrared. |
| Discussion Leader: Sean Crosson (University of Chicago, USA) |
7:30 pm - 7:40 pm | Introduction by Discussion Leader |
7:40 pm - 7:55 pm | Montserrat Elías-Arnanz (Universidad de Murcia, Spain) "Gene Regulation by the B12-Based CarH Photoreceptor: Molecular Mechanism at High Resolution" |
7:55 pm - 8:00 pm | Discussion |
8:00 pm - 8:20 pm | Beronda Montgomery (Michigan State University, USA) "Seeing the Light: Colour Vision and Developmental Acclimation in Cyanobacteria" |
8:20 pm - 8:30 pm | Discussion |
8:30 pm - 8:50 pm | Vladislav Verkhusha (Albert Einstein College of Medicine, USA) "Bacterial Phytochromes for Near-Infrared Imaging and Light-Control in Cells and Animals" |
8:50 pm - 9:00 pm | Discussion |
9:00 pm - 9:20 pm | Sean Crosson (University of Chicago, USA) "Coordinate Control of a Bacterial General Stress Response and Cell Adhesion by Blue Light" |
9:20 pm - 9:30 pm | Discussion |
Thursday |
7:30 am - 8:30 am | Breakfast |
9:00 am - 12:30 pm | Light Regulation of Signaling Networks |
| Plants,
photosynthetic bacteria and algae possess intricate, sophisticated
photoreceptor systems that program their development and photosynthetic
capacity. Understanding how these complex signaling systems are coordinated
represents a major challenge if knowledge is to be used for increasing biomass
production for agronomic gain or for efficient processing of biofuel sources.
This session focuses on the molecular mechanisms associated with regulating
such diverse processes and also integrates synthetic technologies used to place
complex mammalian transcriptional responses under multi-chromatic control. |
| Discussion Leader: Peter Quail (University of California, Berkeley, USA) |
9:00 am - 9:10 am | Introduction by Discussion Leader |
9:10 am - 9:30 am | Karen Halliday (University of Edinburgh, United Kingdom) "Phytochromes Modulate the Switch Between Biomass
Production and Stress Resistance" |
9:30 am - 9:40 am | Discussion |
9:40 am - 10:00 am | Chentao Lin (University of California, Los Angeles, USA) "Photoactivation and Desensitization Mechanisms of Arabidopsis Cryptochromes" |
10:00 am - 10:10 am | Discussion |
10:10 am - 10:25 am | Matias Zurbriggen (University of Düsseldorf, Germany) "Synthetic Light Signaling Switches" |
10:25 am - 10:30 am | Discussion |
10:30 am - 11:00 am | Coffee Break |
11:00 am - 11:15 am | Cheryl Kerfeld (Michigan State University / Lawrence Berkeley National Laboratory, USA) "Structural and Functional Modularity in the Evolution of the Cyanobacterial Orange Carotenoid Protein" |
11:15 am - 11:20 am | Discussion |
11:20 am - 11:35 am | Eirini Kaiserli (University of Glasgow, United Kingdom) "A Novel Integrator of Photomorphogenesis and Photoperiodic Flowering in Nuclear Photobodies" |
11:35 am - 11:40 am | Discussion |
11:40 am - 11:55 am | Enamul Huq (University of Texas at Austin, USA) "Splicing Factor for Light Signal (SFS) Promotes Photomorphogenesis by Regulating pre-mRNA Splicing of ELF3 in Arabidopsis" |
11:55 am - 12:00 pm | Discussion |
12:00 pm - 12:20 pm | Peter Quail (University of California, Berkeley, USA) "Dynamics of the Phy-PIF-Genome Signaling Interface" |
12:20 pm - 12:30 pm | Discussion |
12:30 pm | Lunch |
1:30 pm - 4:00 pm | Free Time |
4:00 pm - 6:00 pm | Poster Session |
6:00 pm | Dinner |
7:30 pm - 9:30 pm | Light Inputs to Biological Rhythms |
| Biological
rhythms are universal in nature but are diverse in their modes of optical
control and molecular circuitry between organisms.
In many cases, signaling
systems interact with one another to achieve complex outcomes. This session will compare and contrast the different modes of
regulatory control found in microbes, fungi, plants and animals. |
| Discussion Leader: Jennifer Loros (Geisel School of Medicine, Dartmouth College, USA) |
7:30 pm - 7:40 pm | Introduction by Discussion Leader |
7:40 pm - 8:00 pm | Stacey Harmer (University of California, Davis, USA) "Interactions Between Circadian and Photoreceptor
Signaling Networks to Produce Complex Behaviours" |
8:00 pm - 8:10 pm | Discussion |
8:10 pm - 8:25 pm | Ethan Buhr (University of Washington, USA) "OPN5-Mediated Photoentrainment of Retinal
Circadian Clocks" |
8:25 pm - 8:30 pm | Discussion |
8:30 pm - 8:50 pm | Michael Rust (University of Chicago, USA) "Seasonal Adaptation of Circadian Clocks In Vitro" |
8:50 pm - 9:00 pm | Discussion |
9:00 pm - 9:20 pm | Jennifer Loros (Geisel School of Medicine, Dartmouth College, USA) "PAS Domain Photoreceptors in Fungi" |
9:20 pm - 9:30 pm | Discussion |
Friday |
7:30 am - 8:30 am | Breakfast |
9:00 am | Departure |