| Session I |
The knock out/null phenotype of RBCM proteins |
| Sunday PM |
7:30 - 9:45 PM |
Chair: Mohandas Narla
- L. Peters (Jackson Labs)
"Protein 4.2 knockout mice"
- Barry Paw (Boston Children's Hospital)
"The zebrafish blood mutation, retsina: a genetic model for erythroid differentiation and dyserythropoiesis"
- A. Chishti (St. Elizabeth Hosp.-Boston)
"p55 knockout mice"
- P. Sims (Scripps Research Institute)
"Phospholipid scramblase: new gene family mediated Ca++-activated transbilayer transfer of plasma membrane phospholipids"
- Diane Gilligan (Jackson Labs)
"Adducin knockout mice"
| Session II |
Membrane structure and mechanics |
| Monday AM |
9:00 AM - 12:30 PM |
Chair: Joel Chasis
- R. McDonald (Northwestern U.)
"X-ray crystal structure of several two motif fragments of spectrin"
- Y. Takakuwa (Tokyo)
"Importance of lipids and their asymmetric distribution on red cell membrane mechanical properties"
- D. Speicher (Wistar Inst.)
"Spectrin functional domains and their regulation"
- R. Korenstein (Tel-Aviv)
"Receptor-mediated regulation of erythrocyte deformability"
- Dachuan Zhang (Purdue University)
"The crystal structure of the cytoplasmic domain of band 3"
- Dennis Discher (Univ. of Pennsylvania)
"Measures of actin orientation (and re-orientation) in the red cell membrane skeleton"
- Holger Schmidt-Schönbein (Univ. of Aachen)
"Membrane Tanktreading of Mammalian non-Nucleated RBC: Molecular Basis of Mechanical Membrane Fluidity in Shear as a Cause of Cellular Adaptivity at Bifurcations and of Macroscopic Viscosity (Pressure Flow Relationship) in Natural Vascular Networks"
| Session III |
Erythroid gene regulation I, LCR function and higher-order gene organization |
| Monday PM |
7:30 - 9:45 PM |
Chair: Mark Groudine
- M. Groudine (F. Hutchinson CRC, Seattle, WA)
"Mechanism of ß-globin locus regulation by the LCR"
- F. Grosveld (Erasmus Univ. Rotterdam, The Netherlands)
"LCR-directed transcription of the ß-globin gene locus"
- Emma Whitelaw (University of Sydney)
"Variable expressivity and epigenetic inheritance in mice"
- D. Bodine (NIH)
"Driving globin gene expression from spectrin and ankyrin gene promoters:
Studies of hemoglobin switching and gene therapy"
- Peter Frasier (Cambridge, UK)
"Developmental activation of chromatin in the human ß-globin locus)"
| Session IV |
Erythroid gene regulation II, regulation of transcription within the globin gene locus |
| Tuesday AM |
9:00 - 12:30 AM |
Chair: Doug Engel
- D. Engel (Northwestern Univ., Evanston, IL)
"Human ß globin locus transcriptional regulation"
- B. Emerson (Salk Institute, San Diego, CA)
"Chromatin remodeling complexes regulate stage-specific human ß-globin activation"
- J. Bieker (Mt. Sinai Sch. Med., New York, NY)
"The role of EKLF in erythroid-specific transcription"
- G. Stamatoyannopoulos (Univ. Washington, Seattle, WA)
"Control of human globin gene switching"
- Jay Chung (NIH)
"PINPOINT view of the EKLF and BRG1 recruitment to the ß-globin promoter"
- Gerd Blobel (Univ. of Pennsylvania)
"Acetylation of transcription factor GATA-1"
- Emery Bresnick (Univ. of Wisconsin)
"Coactivator requirements for ß-globin LCR function"
- John Cunningham (St. Jude's Children's Hospital, Memphis)
"Isolation and characterization of human NF-E4, the fetal-erythroid-specific component of the stage selector protein complex"
| Session V |
Erythrocyte proteins in nonerythroid cells |
| Tuesday PM |
7:30 - 9:45 PM |
Chair: Velia Fowler
- J. Morrow (Yale Univ.)
"Ankyrin in nonerythroid cells"
- V. Bennett (Duke Univ.)
"Adducin and ankyrin in nonerythroid cells"
- Graham Thomas (Penn State)
"Genetic and evolutionary approaches to the non-erythroid membrane skeleton"
- L. Walensky (Johns Hopkins)
"Protein 4.1 and its interaction with immunophilin"
- John Conboy (Lawrence Berkeley Labs)
"Regulation of an alternative splicing switch during erythropoiesis requires coordinated activities of splicing enhancer and silencer elements"
| Session VI |
O2, NO, and Fe+3 in RBC biology |
| Wednesday AM |
9:00 - 12:30 AM |
Chair: Mike Tanner
- J. Stamler (Duke Univ. Medical School)
"Nitric Oxide in the respiratory cycle"
- M. Hentze (Heidelberg)
"Molecular control of iron metabolism by the IRE/IRP regulatory system"
- Randy Sprague (St Louis )
"Communication between red cells and other cells"
- J. Kushner (Univ. Utah)
"Iron uptake and iron export in erythroid and nonerythroid cells"
- Nancy Andrews (Boston Children's Hospital)
"Iron and Erythropoiesis: Lessons from Anemic Mice"
- Robert Hebbel (Univ. of Minnesota)
"Activated endothelial cells: a novel therapeutic target in sickle cell disease"
| Session VII |
Hematopoiesis I: Origin of stem cells and lineage commitment in non-mammalian vertebrates |
| Wednesday PM |
7:30 - 9:45 PM |
Chair: Leonard Zon
- Leonard Zon (Children's Hosp. HHMI, Boston, MA)
"Dissecting Hematopoietic Development Using the Zebrafish"
- F. Dieterlen-Lievre (Institut d'Embryologie du Centre National de la Recherche Scientifique,
Nogent s/Marne, France)
"Hematopoietic and endothelial precursors in the avian allantois"
- J. Turpen (Univ. of Nebraska Med. Centre, Omaha, NE)
"A Role for Maternal Message in Xenopus Hematopoiesis"
- Roger Patient(King's College, London)
"Programming of blood cells before gastrulation in fish and frogs"
- Shuo Lin (Medical College of Georgia) - short
"Transgenic analysis of a novel hematopoietic death gene in zebrafish"
| Session VIII |
Hematopoiesis II: Origin of stem cells and lineage commitment in mammalian systems |
| Thursday AM |
9:00 AM - 12:30 PM |
Chair: Margaret Baron
- M. Baron (Mt. Sinai Sch. Med., New York, NY)
"The role of endoderm-mesoderm signaling in the induction of
embryonic hematopoiesis"
- M. Yoder (Indiana University Sch. Med., Indianapolis, IN)
"Hematopoietic stem cell origin in the early embryonic yolk sac"
- E. Dzierzac (Erasmus Univ., Rotterdam, The Netherlands)
"Hematopoietic stem cell initiation in the mouse embryo"
- S. Orkin (Children's Hosp. HHMI, Boston, MA)
"Regulatory circuitry in hematopoietic development"
- G. Keller (Natl. Jewish Med. & Res. Center, Denver, CO)
"A common precursor for hematopoietic and endothelial cells"
- Jeff Miller (NIH) - short
"Human erythroid cell genomics"
| Session IX |
Gene therapy and other late-breaking developments |
| Thursday PM |
7:00 - 9:30 PM |
Chair: Sam Lux
Keynote Lecture:
Inder Verma (UCSD)
"Gene Therapy: Prospects for the Future"
- K. Haldar (Northwestern University)
"Malarial entry and development in a caveolar invagination induced by lipid-based sorting in the mature red cell"
- Jean-Pierre Cartron (INSERM, Paris)
"Cloning of RBC cell surface antigens"
- David Daleke (Indiana University)
"Effect of diabetes on red cell lipid asymmetry and transport"
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