Sunday evening: | New Challenges for Three-Dimensional Electron Microscopy |
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7:30 | Bridget Carragher, Welcome and chair's remarks. |
7:45 | Robert Glaeser, Technical progress in electron crystallography: Celebrating the present; anticipating the future. |
8:30 | Alasdair Steven, The Devil's in the Details: A Pragmatic Approach to Visualizing Macromolecular Machines at Higher Resolution. |
9:15 | Stephen Harrison, The synergy between 3DEM and X-ray crystallography. |
Monday morning | New Challenges in Specimen Preparation |
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8:45 | Yoshi Fujiyoshi, Opening remarks. |
9:00 | David E. Golan, Membrane Dynamics of the Water Transport Protein Aquaporin-1 in Intact Human Red Cells. |
9:20 | Daniel Levy, 2D crystallization of membrane proteins on lipid layers. |
9:50 | Elizabeth Wilson-Kubalek, Helical crystallization of proteins on preformed lipid tubules. |
10:10 | Michael Stowell, In search of tubular crystallization templates for membrane proteins. |
10:30 | Coffee |
11:00 | David Belnap, The Use of Biological Labels to Understand Poliovirus Cell-Entry. |
11:30 | Seth Darst, RNA polymerase: novel methods for locating functional sites. |
Monday evening: | Alternative Techniques and New Approaches |
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7:30 | Stephen Fuller, Opening remarks. |
7:45 | Alain Brisson, Observing dynamic changes in lipid monolayer crystals using AFM. |
8:15 | Jaques Dubochet, Cell and molecular structures from vitreous sections. |
8:45 | John Heuser, Deep-etch images. |
9:15 | Roger Wepf, Cryo-AFM and cryo-SEM reveals the beauty of macromolecular complexes in situ. |
Tuesday morning | New Challenges in Resolution |
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8:30 | Group photograph |
8:45 | David Stokes, Opening remarks. |
9:00 | Nigel Unwin, Acetylcholine receptor at 4.6 A resolution. |
9:30 | Nikolau Gregorieff, Electron Microscopy of Mtochondrial Complex I |
10:00 | Karen Williams, 3D structure of NhaA, a bacterial Na+/H+ antiporter at 7 Å resolution. |
10:30 | Coffee |
11:00 | Erika Mancini, SFV: Viewing a fusion machine at higher resolution |
11:30 | Wah Chiu, Herpesvirus capsid structure. |
Tuesday afternoon: | Computer Workshop Short Presentations |
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| Gina Sosinsky, Michael Schatz |
Tuesday evening: | Short platform presentations of selected posters: new technical methods |
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7:30 | Esther Bullitt, Opening remarks. |
Wednesday morning: | New Challenges in Imaging and Data Acquisition |
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8:45 | Michael Radermacher, Opening remarks. |
9:00 | Chikashi Toyoshima, Electron crystallography of thin protein crystals |
9:30 | Clint Potter, Automated data collection for cryoEM. |
10:00 | Brent Gowen, The First Single-Particle Results from the Philips' CM300-FEG-Helium Microscope |
10:20 | Coffee. |
10:40 | Carmen Manella, Cryo-tomography of whole mitochondria. |
11:00 | Teresa Riuz, The Centrosome: The Three-dimensional Structure of a Cell Organelle at 40 A Resolution. |
11:30 | Technical round table. |
Wednesday evening: | Short platform presentations of selected posters: new biological results |
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7:30 | Elizabeth Hewat, Opening remarks. |
Thursday morning: | New Challenges in Analysis and Interpretation |
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8:45 | Eva Nogales, Opening remarks. |
9:00 | Yizhi Tao, Morphogenesis of bacteriophage phi 29: from start to finish and more. |
9:30 | Niels Volkmann, Hybrid Crystallography using Correlative Fitting Distributions. |
9:50 | Dorit Hanein, Conformational variability in actomyosin complexes. |
10:20 | Coffee |
10:50 | Rajendra Agrawal, Cryo-electron microscopy and interpretation of functional complexes of the ribosome. |
11:20 | Willy Wriggers, Signal processing of multi-resolution structural data. |
11:40 | Jenny Hinshaw, Structural studies of dynamin help elucidate its role in membrane recycling. |
Thursday afternoon | Scanner Workshop |
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| Linda Melanson and Nicolau Gregorieff |
| Alastair Stuart, Klaus Neuman, Poul Kjear, Zeiss |
Thursday evening | Late-breaking results and final panel discussion |
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7:30 | Bridget Carragher and Bob Glaeser, Business meeting. |
8:14 | David DeRosier, Opening remark. |
8:15 | Holger Stark, The 3D Arrangement of RNA and Proteins in the Spliceosomal U1 snRNP. |
8:45 | Masahide Kikkawa, 15A Resolution Model of the Monomeric Kinesin Motor : KIF1A Head- Microtubule Complex |
9:15 | Stephan Nickell, Electron Tomography of Cellular Specimens |
9:45 | Open discussion on the future of 3DEM. |