| SUNDAY |
| 2:00 pm - 9:00 pm | Arrival and Check-in |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | 3DEM - THE CHALLENGE OF BRIDGING CELLULAR AND ATOMIC RESOLUTION |
| Discussion Leader: Kenneth Taylor (Florida State University) |
| 7:30 pm - 7:40 pm | Introduction and overview |
| 7:40 pm - 8:15 pm | Charles Brooks III (Scripps)
Dynamic modeling of low to medium resolution structural data using Gaussian network normal mode analysis |
| 8:15 pm - 8:35 pm | Discussion |
| 8:35 pm - 9:10 pm | Steven C. Almo (Albert Einstein)
The shape of things to come: Structural Genomics and Structural Proteomics |
| 9:10 pm - 9:30 pm | Discussion |
| MONDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | HIGH RESOLUTION STRUCTURES FROM SINGLE PARTICLES AND HELICAL ASSEMBLIES |
| Discussion Leader: Eva Nogales (UC Berkeley) |
| 9:00 am - 9:10 am | Introduction and overview |
| 9:10 am - 9:35 am | Bettina Böttcher (EMBL-Heidelberg)
Icosahedral scaffolding for structure determination |
| 9:35 am - 9:50 am | Discussion |
| 9:50 am - 10:15 am | Holger Stark (MPI Göttingen)
3D structures of spliceosomal complexes and snRNPs |
| 10:15 am - 10:30 am | Discussion |
| 10:30 am - 11:10 am | Coffee Break and Group Photo |
| 11:10 am - 11:35 am | Yifan Cheng (Harvard Medical School)
Cryo-electron Microscopy of the Human Transferrin Receptor - Transferrin Complex |
| 11:35 am - 11:50 am | Discussion |
| 11:50 am - 12:15 pm | Z Hong Zhou (UT - Houston Med. School)
Resolving the Protein Folds in Cytoplasmic Polyhedrosis Virus |
| 12:15 pm - 12:30 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | HIGH RESOLUTION FROM 2-D ARRAYS |
| Discussion Leader: Yoshi Fujiyoshi (Kyoto University) |
| 7:30 pm - 7:40 pm | Introduction and overview |
| 7:40 pm - 8:05 pm | Christine Ziegler (Max Planck Institute of Biophysics)
Structure and function of the osmoregulating sodium/glycine betaine symporter BetP of Corynebacterium glutamicum |
| 8:05 pm - 8:20 pm | Discussion |
| 8:20 pm - 8:45 pm | Kaoru Mitsuoka (Kyoto University)
2D crystallization and structural analysis of aquaporins |
| 8:45 pm - 9:00 pm | Discussion |
| 9:00 pm - 9:25 pm | Nigel Unwin (MRC Lab. of Molecular Biology, Cambridge)
Atomic model of the ACh receptor pore obtained from images of tubes |
| Discussion |
| TUESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | NEW RECONSTRUCTION TOOLS |
| Discussion Leader: David DeRosier (Brandeis) |
| 9:00 am - 9:10 am | Introduction and overview |
| 9:10 am - 9:35 am | Ed Egelman (U. Virginia)
A New Approach to Reconstructing Helical Filaments and Complexes |
| 9:35 am - 9:50 am | Discussion |
| 9:50 am - 10:15 am | Rasmus Schroeder (Max Planck-Heidelberg)
High resolution reconstruction of helical objects: 1 - least squares solution to axial tomography 2 - determination of projection angles by MSA |
| 10:15 am - 10:30 am | Discussion |
| 10:30 am - 11:00 am | Coffee Break |
| 11:00 am - 11:25 am | Tanvir Shaikh (NYSDH)
The free FSC: Examination of model bias in EM reconstructions using cross-validation |
| 11:25 am - 11:40 am | Discussion |
| 11:40 am - 12:05 pm | Niels Volkmann (Burnham Institute)
Interpreting EM maps: noise-reduction, segmentation, pattern recognition |
| 12:05 pm - 12:20 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | SELECTED POSTERS ON ELECTRON TOMOGRAPHY AND SINGLE PARTICLE ANALYSIS |
| Discussion Leader: Gina Sosinsky (UCSD Medical School) |
| TBN (8 speakers, 10 min presentation and 5 min discussion each) |
| WEDNESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | QUANTITATIVE METHODS FOR BUILDING, REFINING AND TESTING HYBRID STRUCTURES |
| Discussion Leader: Willy Wriggers (Scripps) |
| 9:00 am - 9:10 am | Introduction and overview |
| 9:10 am - 9:35 am | Wen Jiang (Baylor College of Medicine)
Fold and Structural Maturation of Bacteriophage P22 |
| 9:35 am - 9:50 am | Discussion |
| 9:50 am - 10:15 am | Michael Chapman (FSU)
Fitting known structures to EM maps - real-space refinement with stereochemical restraints |
| 10:15 am - 10:30 am | Discussion |
| 10:30 am - 11:00 am | Coffee Break |
| 11:00 am - 11:25 am | Dmitri Svergun (EMBL-Hamburg)
Analysis of quaternary structure of biological macromolecules with small-angle X-ray and neutron scattering |
| 11:25 am - 11:40 am | Discussion |
| 11:40 am - 12:05 pm | Andrej Sali (UCSF)
Modeling the structures of macromolecular assemblies by satisfaction of spatial restraints |
| 12:05 pm - 12:20 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:00 pm - 7:30 pm | Business Meeting |
| 7:30 pm - 9:30 pm | INSTRUMENTAL ADVANCES |
| Discussion Leader: David Agard (UCSF) |
| 7:30 pm - 7:40 pm | Introduction and overview |
| 7:40 pm - 8:05 pm | Ken Downing (UC Berkeley)
Experience with an electron decelerator for improved CCD performance in intermediate voltage electron microscopy |
| 8:05 pm - 8:20 pm | Discussion |
| 8:20 pm - 8:45 pm | Paul Midgley (University of Cambridge)
Z-contrast STEM HAADF Tomography |
| 8:45 pm - 9:00 pm | Discussion |
| 9:00 pm - 9:25 pm | Jacques Dubochet (Université de Lausanne)
Cryoelectron Microscopy of Frozen, Hydrated Thin Tissue Sections |
| Discussion |
| THURSDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | ELECTRON TOMOGRAPHY |
| Discussion Leader: Esther Bullitt (Boston University School of Medicine) |
| 9:00 am - 9:10 am | Introduction and overview |
| 9:10 am - 9:35 am | Daniela Nicastro (U Colorado, Boulder)
Cryo-electron tomography of quiescent and activated eukaryotic flagella |
| 9:35 am - 9:50 am | Discussion |
| 9:50 am - 10:15 am | Ohad Medalia (MPI Martinsried)
3-D actin networks in intact cells revealed by cryo-electron tomography |
| 10:15 am - 10:30 am | Discussion |
| 10:30 am - 11:00 am | Coffee Break |
| 11:00 am - 11:25 am | Sriram Subramanian (NIH)
Three-dimensional imaging of chemotaxis receptor networks in a bacterial cell |
| 11:25 am - 11:40 am | Discussion |
| 11:40 am - 12:05 pm | Sharon G. Wolf (Weizmann Institute of Science)
Chromatin protection by physical sequestration in starved bacteria: A structural study of intracellular protein/DNA ordered assemblies |
| 12:05 pm - 12:20 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | SELECTED POSTERS: STRUCTURAL ANALYSIS OF ORDERED ASSEMBLIES |
| Discussion Leader: Vinzenz Unger (Yale University) |
| TBN (speakers, 10 min presentation and 5 min discussion each) |
| FRIDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am | Depart |
