| SUNDAY |
| 4:00 pm - 9:00 pm | Arrival and Check-in |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | INTEGRATION FROM CELLS TO THE PDB |
| 7:30 pm - 7:40 pm | Discussion Leader: Helen Saibil (Birkbeck College London)
Welcome and Introduction |
| 7:40 pm - 8:15 pm | Jacques Dubochet (Université de Lausanne)
"Will CryoEM fulfill its promise for Cell Biology?" |
| 8:15 pm - 8:35 pm | Discussion |
| 8:35 pm - 9:10 pm | Helen Berman (Rutgers University)
"How the History of the Protein Databank can inform the Future of Structural Biology" |
| 9:10 pm - 9:30 pm | Discussion |
| MONDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | SINGLE PARTICLES |
| 9:00 am - 9:10 am | Discussion Leader: Jose Carrascosa (Centro Nacional de Biotecnologia, Madrid)
Introduction and overview |
| 9:10 am - 9:35 am | Yifan Cheng (Harvard Medical School)
"Structures of proteasome-substrate Complexes" |
| 9:35 am - 9:50 am | Discussion |
| 9:50 am - 10:15 am | Elena Orlova (Birkbeck College London)
"Structure of p53 Tumour Suppressor Protein" |
| 10:15 am - 10:30 am | Discussion |
| 10:30 am - 11:10 am | Coffee Break / Group Photo |
| 11:10 am - 11:35 am | Bjorn Sander (Max Planck Institute for Biophysical Chemistry, Gottingen)
"Structural organization of the core spliceosomal component U4/U6.U5 tri-snRNP" |
| 11:35 am - 11:50 am | Discussion |
| 11:50 am - 12:15 pm | Derek Taylor (HHMI Wadsworth Center, Albany)
"Conformational changes in ribosome-bound eukaryotic elongation factor 2 induced by GTP hydrolysis" |
| 12:15 pm - 12:30 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 5:30 pm | Poster Session |
| 5:30 pm - 7:30 pm | ORDERED AND ICOSAHEDRAL ASSEMBLIES |
| 5:30 pm - 5:40 pm | Discussion Leader: David Stokes (Skirball Institute, NY)
Introduction and overview |
| 5:40 pm - 6:05 pm | Sarah Butcher (University of Helsinki)
"Salt of the earth, the archaeal virus SH1 challenge to cryo-EM and image processing" |
| 6:05 pm - 6:15 pm | Discussion |
| 6:15 pm - 6:40 pm | Vinoth Kumar (MRC Laboratory of Molecular Biology, Cambridge, UK)
"Conformational changes in sodium/proton antiporters: structure and mechanisms" |
| 6:40 pm - 6:50 pm | Discussion |
| 6:50 pm - 7:15 pm | Ed Egelman (University of Virginia)
"Order and Disorder in Helical Polymers: Insights from the IHRSR Method" |
| 7:15 pm - 7:30 pm | Discussion |
| 8:00 pm | Dinner |
| TUESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | RECONSTRUCTION METHODS AND HETEROGENEITY |
| 9:00 am - 9:10 am | Discussion Leader: Joachim Frank (Howard Hughes Medical Center, Albany)
Introduction and overview |
| 9:10 am - 9:35 am | Andres Leschziner (University of California, Berkeley)
"Getting more out of your tilt: addressing heterogeneity with the orthogonal tilt and random conical tilt methods" |
| 9:35 am - 9:50 am | Discussion |
| 9:50 am - 10:15 am | Sjors Scheres (Centro Nacional de Biotecnologia, Madrid)
"Treating Structural Heterogeneity using Maximum-likelihood Refinement" |
| 10:15 am - 10:30 am | Discussion |
| 10:30 am - 11:10 am | Coffee Break |
| 11:10 am - 11:35 am | Bruno Klaholz (Institut de Genetique et de Biologie Moleculaire et Cellulaire, France)
"Local multivariate statistical analysis in 2D and 3D to handle structural heterogeneity" |
| 11:35 am - 11:50 am | Discussion |
| 11:50 am - 12:15 pm | Marina Mapelli (FIMO - FIRC Institute of Molecular Oncology, Italy)
"Strategies for in vitro reconstitution of multi-component protein complexes" |
| 12:15 pm - 12:30 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 5:30 pm | Poster Session |
| 5:30 pm - 7:30 pm | POSTER PRESENTATIONS AND DISCUSSION I |
| Discussion Leader: Werner Kullbrandt (Max Planck Institute for Biophysics, Frankfurt) |
| 8 speakers to be chosen from poster presenters, 10 min presentation and 5 min discussion each. |
| 8:00 pm | Dinner |
| WEDNESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | TOMOGRAPHY AND CELL BIOLOGY |
| 9:00 am - 9:10 am | Discussion Leader: Ken Taylor (Florida State University)
Introduction and overview |
| 9:10 am - 9:35 am | Ohad Medalia (Ben-Gurion University, Beer-sheva)
"Improving the Resolution of Nuclear Pore Complex from Tomograms of Intact Nuclei" |
| 9:35 am - 9:50 am | Discussion |
| 9:50 am - 10:15 am | Kay Grünewald (Max Planck Institute of Biochemistry, Munich)
"Studying Cell Biology of Virus Infection by Electron Cryo Tomography" |
| 10:15 am - 10:30 am | Discussion |
| 10:30 am - 11:10 am | Coffee Break |
| 11:10 am - 11:35 am | Feng Ye (Florida State University)
"Visualizing conformational changes of integral membrane protein in a membrane environment by cryoelectron tomography" |
| 11:35 am - 11:50 am | Discussion |
| 11:50 am - 12:15 pm | Catarina Gadelha (University of Oxford)
"The trypanosome flagellar pocket: cytoarchitecture critical for cell morphogenesis and parasitism" |
| 12:15 pm - 12:30 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 5:30 pm | Poster Session |
| 5:30 pm - 7:30 pm | INSTRUMENTATION |
| 5:30 pm - 5:40 pm | Discussion Leader: Richard Henderson (Medical Research Council Laboratory of Molecular Biology, Cambridge)
Introduction and overview |
| 5:40 pm - 6:05 pm | Wasi Faruqi (Medical Research Council Laboratory of Molecular Biology, Cambridge)
"Semiconductor pixel detectors for electron microscopy" |
| 6:05 pm - 6:15 pm | Discussion |
| 6:15 pm - 6:40 pm | Rasmus Schroeder (Max Planck Institute for Biophysics, Frankfurt)
"Using an electrostatic (Boersch) phase plate for optimizing phase contrast" |
| 6:40 pm - 6:50 pm | Discussion |
| 6:50 pm - 7:15 pm | Rossana Cambie (Lawrence Berkeley National Laboratory)
"Design of a Microfabricated, Two-Electrode Phase-Contrast Element Suitable for Electron Microscopy" |
| 7:15 pm - 7:30 pm | Discussion |
| 7:30 pm - 8:00 pm | Business Meeting |
| (Nominations for the next Vice Chair; Fill out Conference Evaluation Forms; Discuss future Site & Scheduling preferences; Election of the next Vice Chair) |
| 8:00 pm | Dinner |
| THURSDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | HYBRID STRUCTURES AND DATABASES |
| 9:00 am - 9:10 am | Discussion Leader: Wah Chiu (Baylor College)
Introduction and overview |
| 9:10 am - 9:35 am | Kim Henrick (European Bioinformatics Institute)
"Macromolecular Structure Databases and Data Management" |
| 9:35 am - 9:50 am | Discussion |
| 9:50 am - 10:15 am | Florence Tama (University of Arizona)
"Flexible High-resolution of Cryo-EM Data using Normal Mode Analysis" |
| 10:15 am - 10:30 am | Discussion |
| 10:30 am - 11:10 am | Coffee Break |
| 11:10 am - 11:35 am | Maya Topf (California Institute for Quantitative Biomedical Research)
"Refinement of Protein Structures by Combining Comparative Modeling and CryoEM Density Fitting" |
| 11:35 am - 11:50 am | Discussion |
| 11:50 am - 12:15 pm | Trevor Sewell (University of Cape Town)
"Nitrilases: Versatile Helix Formers" |
| 12:15 pm - 12:30 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 5:30 pm | Poster Session |
| 5:30 pm - 7:30 pm | POSTER PRESENTATIONS AND DISCUSSION II |
| Discussion Leader: Phoebe Stewart (Vanderbilt University) |
| 8 speakers to be chosen from poster presenters, 10 min presentation and 5 min discussion each. |
| 8:00 pm | Dinner |
| FRIDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am | Depart |
