| SUNDAY |
| 2:00 pm - 11:00 pm | Arrival and Check-in |
| 6:00 pm | Dinner |
| 7:30 pm - 7:40 pm | Welcome / Introductory Comments by GRC Site Staff |
| 7:40 pm - 9:30 pm | VISUALIZING BIOLOGICAL PROCESSES WITH 3D EM |
| 7:40 pm - 7:50 pm | Discussion Leader: Phoebe Stewart (Vanderbilt University Medical Center)
Introduction and Overview |
| 7:50 pm - 8:25 pm | Tony Crowther (MRC Laboratory of Molecular Biology)
"Cryomicroscopy of Viruses: Variations on a Theme" |
| 8:25 pm - 8:40 pm | Discussion |
| 8:40 pm - 9:15 pm | Ron Milligan (The Scripps Research Institute)
"Visualizing Mechanochemical Mechanisms in the Kinesins" |
| 9:15 pm - 9:30 pm | Discussion |
| MONDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | RNA AND RIBOSOME TRANSACTIONS |
| 9:00 am - 9:10 am | Discussion Leader: Michael Radermacher (University of Vermont)
Introduction and Overview |
| 9:10 am - 9:35 am | Jean-Francois Menetret (Boston University School of Medicine)
"Ribosome Binding of a Single Copy of the Secy/Sec61 Complex: Implications for the Initiation of Protein Translocation" |
| 9:35 am - 9:50 am | Discussion |
| 9:50 am - 10:15 am | Jan Giesebrecht (Charité - Universitätsmedizin Berlin)
"Analysis of Functional 80S Intermediates of the Mammalian Ribosomal Elongation Cycle by Multi-Particle Cryo-EM" |
| 10:15 am - 10:30 am | Discussion |
| 10:30 am | Coffee Break / Group Photo |
| 11:10 am - 11:35 am | Gregory Allen (HHMI / Wadsworth Center)
"Cryo-EM of a Chimeric Ribosome-Group II Intron Complex: a General Method for Imaging Large, Structured RNAs" |
| 11:35 am - 11:50 am | Discussion |
| 11:50 am - 12:15 pm | B.V.V. Prasad (Baylor College of Medicine)
"Structural Insights into Rotavirus Replication" |
| 12:15 pm - 12:30 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | DNA TRANSACTIONS |
| 7:30 pm - 7:40 pm | Discussion Leader: Niko Grigorieff (HHMI / Brandeis University)
Introduction and Overview |
| 7:40 pm - 8:05 pm | Francisco Asturias (The Scripps Research Institute)
"Structure of a RSC-Nucleosome Complex and Implications for the Chromatin Remodeling Mechanism" |
| 8:05 pm - 8:15 pm | Discussion |
| 8:15 pm - 8:40 pm | Arnaud Amzallag (Ecole Polytechnique Fédérale de Lausanne)
"3D Reconstruction and Analysis of Shapes of DNA Minicircles Observed by Cryo-Electron Microscopy" |
| 8:40 pm - 8:50 pm | Discussion |
| 8:50 pm - 9:15 pm | Takayuki Kato (Osaka University)
"Structural Analysis of DNA Tetrahedron" |
| 9:15 pm - 9:30 pm | Discussion |
| TUESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | ISOLATED PROTEINS AND PROTEIN FILAMENTS |
| 9:00 am - 9:10 am | Discussion Leader: Daniela Nicastro (Brandeis University)
Introduction and Overview |
| 9:10 am - 9:35 am | Deborah Kelly (Harvard Medical School)
"Monolayer Purification: A Rapid Method for Isolating Protein Complexes for Single Particle EM" |
| 9:35 am - 9:50 am | Discussion |
| 9:50 am - 10:15 am | Charles Sindelar (Lawrence Berkeley National Laboratory)
"Nanometer-Resolution Studies of Human Conventional Kinesin Complexed to the Microtubule" |
| 10:15 am - 10:30 am | Discussion |
| 10:30 am | Coffee Break |
| 11:10 am - 11:35 am | Grant Jensen (California Institute of Technology)
"Electron Cryotomography of the Bacterial Cytoskeleton" |
| 11:35 am - 11:50 am | Discussion |
| 11:50 am - 12:15 pm | Dorit Hanein (The Burnham Institute)
"Three-Dimensional Structure of the Arp2/3 Branch Junction" |
| 12:15 pm - 12:30 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | POSTER PRESENTATIONS AND DISCUSSION I |
| Discussion Leader: Ingeborg Schmidt-Krey (Georgia Institute of Technology) |
| TBA (8 speakers, 10 min presentation and 5 min discussion each) |
| WEDNESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | HIGHER ORDER ASSEMBLIES |
| 9:00 am - 9:10 am | Discussion Leader: James Conway (University of Pittsburgh School of Medicine)
Introduction and Overview |
| 9:10 am - 9:35 am | Beate Rockel (Max Planck Institute of Biochemistry)
"Electron Microscopy of a Giant Protease" |
| 9:35 am - 9:50 am | Discussion |
| 9:50 am - 10:15 am | Jack Johnson (The Scripps Research Institute)
"Late Stage HK97 Virus Particle Maturation: Visualizing a Brownian Ratchet with Crystallography and CryoEM" |
| 10:15 am - 10:30 am | Discussion |
| 10:30 am | Coffee Break |
| 11:10 am - 11:35 am | Thomas Marlovits (IMP-IMBA Research Center, Austrian Academy of Sciences)
"Reprogramming the Type III Injectisome" |
| 11:35 am - 11:50 am | Discussion |
| 11:50 am - 12:15 pm | Steven Ludtke (Baylor College of Medicine)
"Backbone Trace and Dynamics of GroEL by Single Particle Reconstruction" |
| 12:15 pm - 12:30 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:00 pm - 7:30 pm | Business Meeting |
| (Nominations and election for the Chair of the 2011 meeting in the US, who will also be the Poster Chair for the 2009 meeting in the US; Fill out Conference Evaluation Forms; Discuss future Site & Scheduling preferences) |
| 7:30 pm - 9:30 pm | MEMBRANE PROTEINS AND MEMBRANE PROXIMAL EVENTS |
| 7:30 pm - 7:40 pm | Discussion Leader: Werner Kuehlbrandt (Max Planck Institute of Biophysics)
Introduction and Overview |
| 7:40 pm - 8:05 pm | Bernard Heymann (National Institute of Arthritis and Musculoskeletal and Skin Diseases)
"Cryo-Electron Tomography of Coated Vesicles: Distribution of Particle Forms and Defining their Components" |
| 8:05 pm - 8:15 pm | Discussion |
| 8:15 pm - 8:40 pm | Mark Yeager (The Scripps Research Institute)
"Structure of Full-length HIV-1 CA by Electron Cryo-Crystallography: A Model for the Mature Capsid Lattice" |
| 8:40 pm - 8:50 pm | Discussion |
| 8:50 pm - 9:15 pm | Stephen Fuller (Wellcome Trust Centre for Human Genetics)
"Cryo-Electron Microscopy Provides Insights into how HIV Cheats at Assembly and Infection" |
| 9:15 pm - 9:30 pm | Discussion |
| THURSDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | TECHNICAL ADVANCES |
| 9:00 am - 9:10 am | Discussion Leader: Bridget Carragher (The Scripps Research Institute)
Introduction and Overview |
| 9:10 am - 9:35 am | Jens Meiler (Vanderbilt University)
"EM-Fold: A Novel Approach for Protein Structure Elucidation from Cryo-EM Density Maps" |
| 9:35 am - 9:50 am | Discussion |
| 9:50 am - 10:15 am | Radostin Danev (Okazaki Institute for Integrative Bioscience)
"Single Particle Analysis Based on Zernike Phase Contrast TEM" |
| 10:15 am - 10:30 am | Discussion |
| 10:30 am | Coffee Break |
| 11:10 am - 11:35 am | Harald Rose (Technical University Darmstadt)
"Criteria and Prospects for Realizing Optimum Electron Microscopes" |
| 11:35 am - 11:50 am | Discussion |
| 11:50 am - 12:15 pm | Sriram Subramaniam (National Cancer Institute)
"3D Imaging of Mammalian Cells with Ion-Abrasion Scanning Electron Microscopy" |
| 12:15 pm - 12:30 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | POSTER PRESENTATIONS AND DISCUSSION II |
| Discussion Leader: Esther Bullitt (Boston University School of Medicine) |
| TBA (8 speakers, 10 min presentation and 5 min discussion each) |
| FRIDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am | Depart |
