| SUNDAY |
| 2:00 pm - 9:00 pm | Arrival and Check-in (Office Closed 6:00 pm - 7:00 pm) |
| 6:00 pm | Dinner |
| 7:30 pm - 7:40 pm | Welcome / Introductory Comments by GRC Site Staff |
| 7:40 pm - 9:30 pm | Imaging and Information |
| 7:40 pm - 7:50 pm | Discussion Leader: Michael Radermacher (University of Vermont, Burlington,VT)
"Introduction and Overview" |
| 7:50 pm - 8:25 pm | Robert Glaeser (Lawrence Berkeley National Laboratory, Berkeley, CA)
"Reaching the Information Limit in Cryo-EM of Biological Macromolecules - Experimental Aspects" |
| 8:25 pm - 8:40 pm | Discussion |
| 8:40 pm - 9:15 pm | Reiner Hegerl (Max-Planck-Institute for Biochemistry, Martinsried, Germany)
"Structural Information: A new Criterion for Image Quality? An Attempt" |
| 9:15 pm - 9:30 pm | Discussion |
| MONDAY |
| 7:30 am - 8:30 am | Breakfast |
| 8:30 am | Group Photo |
| 9:00 am - 12:30 pm | 3D Reconstruction of Single Particles, Methods and Results I |
| 9:00 am - 9:10 am | Discussion Leader: John Rubinstein (The Hospital for Sick Children, Toronto, Canada)
"Introduction and Overview" |
| 9:10 am - 9:35 am | Giovanni Cardone (NIH, Laboratory of Structural Biology Research, Bethesda, MD)
"Local Variation in Resolution in Cryo-EM Density Maps" |
| 9:35 am - 9:50 am | Discussion |
| 9:50 am - 10:30 am | Coffee Break |
| 10:30 am - 10:55 am | David Barford (Institute of Cancer Research, London, UK)
"Structural Basis for the Subunit Assembly of the Anaphase Promoting Complex" |
| 10:55 am - 11:10 am | Discussion |
| 11:10 am - 11:35 am | Teresa Ruiz (University of Vermont, Burlington, VT)
"High Resolution Structure of S. cerevisiae Phosphofructokinase: A Combination of Cryo 3D Reconstruction, Modeling and Docking" |
| 11:35 am - 11:50 am | Discussion |
| 11:50 am - 12:15 pm | John Rubinstein (The Hospital for Sick Children, Toronto, Canada)
"Cryo-EM Map Refinement for Small Membrane Protein Complexes" |
| 12:15 pm - 12:30 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | Multiresolution: Fitting of X-ray Models to 3D EM Data |
| 7:30 pm - 7:45 pm | Discussion Leader: Edward Morris (Institute of Cancer Research, London, UK)
"Introduction and Overview" |
| 7:45 pm - 8:10 pm | Tom Goddard (University of California, San Francisco, CA)
"Packing Macromolecules in Medium Resolution (10-20Å) Maps with UCSF Chimera" |
| 8:10 pm - 8:20 pm | Discussion |
| 8:20 pm - 8:45 pm | Michael S. Chapman (Oregon Health & Science University, Portland, OR)
"Hybrid Structure Refinement: Choosing the Algorithm for Greatest Precision" |
| 8:45 pm - 8:55 pm | Discussion |
| 8:55 pm - 9:20 pm | Karen Lasker (University of California, San Francisco, CA)
"Structural Modeling of Macromolecular Assemblies by Alignment of Interaction Networks to Electron Microscopy Density Maps" |
| 9:20 pm - 9:30 pm | Discussion |
| TUESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | 3D Structure Analysis of Ordered Assemblies |
| 9:00 am - 9:20 am | Discussion Leader: Ingeborg Schmidt-Krey (Georgia Institute of Technology, Atlanta GA)
"Introduction and Overview" |
| 9:20 am - 9:45 am | Yoshinori Fujiyoshi (Kyoto University, Kyoto, Japan)
"Strong Points of Electron Crystallography Shown in Water and Ion Channels" |
| 9:45 am - 10:00 am | Discussion |
| 10:00 am - 10:30 am | Coffee Break |
| 10:30 am - 10:55 am | Tamir Gonen (University of Washington, Seattle, WA)
"Fragment Based Phase Extension in Electron Crystallography" |
| 10:55 am - 11:10 am | Discussion |
| 11:10 am - 11:35 am | Keiichi Namba (Osaka University, Osaka, Japan)
"High-resolution High-throughput Helical Image Analysis of Macromolecular Assemblies by Cryo-EM with In-Column Energy Filter and CCD" |
| 11:35 am - 11:50 am | Discussion |
| 11:50 am - 12:15 pm | Phoebe Stewart (Vanderbilt University, Nashville, TN)
"CryoEM Visualization and Modeling of Inserted Epitopes and Bound Peptides on the Adenovirus Capsid" |
| 12:15 pm - 12:30 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | Poster Presentations and Discussion I |
| Discussion Leader: Eva Nogales (University of California, Berkeley, CA) |
| TBA (8 speakers, 10 min presentation and 5 min discussion each) |
| WEDNESDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | 3D Reconstruction of Single Particles, Methods and Results II |
| 9:00 am - 9:20 am | Discussion Leader: Andres Leschziner (Harvard University, Cambridge, MA)
"Introduction and Overview" |
| 9:20 am - 9:45 am | Pawel Penczek (The University of Texas - Houston Medical School, Houston, TX)
"Stability and Reproducibility: an Objective Approach to 2D Alignment and Clustering" |
| 9:45 am - 10:00 am | Discussion |
| 10:00 am - 10:30 am | Coffee Break |
| 10:30 am - 10:55 am | Thomas Marlovits (Institute of Molecular Biotechnology, Austrian Academy of Sciences, Vienna, Austria)
"Dissecting the Injectisome for Sub-nanometer Resolution Structure Determination" |
| 10:55 am - 11:10 am | Discussion |
| 11:10 am - 11:35 am | Robert Langlois (Columbia University, New York, NY)
"Comparing the performance of semi-automated particle picking algorithms -- a clarification of machine-learning terms" |
| 11:35 am - 11:50 am | Discussion |
| 11:50 am - 12:15 pm | Helen Saibil (Birkbeck College, London, UK)
"Trajectory of Chaperonin Machine Movements Reconstructed from a Single Particle Data Set" |
| 12:15 pm - 12:30 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:00 pm - 7:30 pm | Business Meeting |
| Nominations for the next Vice Chair; Fill out Conference Evaluation Forms; Discuss future Site & Scheduling preferences; Election of the next Vice Chair |
| 7:30 pm - 9:30 pm | New Results with Recent Techniques |
| 7:30 pm - 7:45 pm | Discussion Leader: Anchi Cheng (The Scripps Research Institute, La Jolla, CA)
"Introduction and Overview" |
| 7:45 pm - 8:10 pm | Yannik Schwab (IGBMC, Illkirch, France)
"Correlative Light and Electron Microscopy: From Live Imaging to 3D Ultrastructure" |
| 8:10 pm - 8:20 pm | Discussion |
| 8:20 pm - 8:45 pm | Wah Chiu (Baylor College of Medicine, Houston, TX)
"Zernike Phase Contrast Cryo-EM Reveals Previously Unseen Structural Features in Nanomachines" |
| 8:45 pm - 8:55 pm | Discussion |
| 8:55 pm - 9:20 pm | Daniel Wolf (Technical University Dresden, Dresden, Germany)
"Holographic Electron Tomography: 3D Imaging Using Direct Phase Contrast" |
| 9:20 pm - 9:30 pm | Discussion |
| THURSDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am - 12:30 pm | Electron Tomography |
| 9:00 am - 9:20 am | Discussion Leader: Esther Bullit (Boston University School of Medicine, Boston, MA)
"Introduction and Overview" |
| 9:20 am - 9:45 am | Scott Stagg (Florida State University, Tallahassee, FL)
"Dealing with Heterogeneity in the COPII Coat" |
| 9:45 am - 10:00 am | Discussion |
| 10:00 am - 10:30 am | Coffee Break |
| 10:30 am - 10:55 am | Salvatore Lanzavecchia (Università degli Studi di Milano, Milan, Italy)
"3D Alignment and Averaging of Quasi Equivalent Volumes Extracted from Tomograms" |
| 10:55 am - 11:10 am | Discussion |
| 11:10 am - 11:35 am | Elisabeth Villa (Max-Planck-Institute for Biochemistry, Martinsried, Germany)
"Revealing the Molecular Architecture of the Nuclear Pore Complex using Cryo-Electron Tomography" |
| 11:35 am - 11:50 am | Discussion |
| 11:50 am - 12:15 pm | Daniela Nicastro (Brandeis University, Waltham, MA)
"Deconstructing Cilia and Flagella Function with Cryo-Electron Tomography and Structural Proteomics" |
| 12:15 pm - 12:30 pm | Discussion |
| 12:30 pm | Lunch |
| 1:30 pm - 4:00 pm | Free Time |
| 4:00 pm - 6:00 pm | Poster Session |
| 6:00 pm | Dinner |
| 7:30 pm - 9:30 pm | Poster Presentations and Discussion II |
| Discussion Leader: Montserrat Samsó (Virginia Commonwealth University, Richmond, VA) |
| TBA (8 speakers, 10 min presentation and 5 min discussion each) |
| FRIDAY |
| 7:30 am - 8:30 am | Breakfast |
| 9:00 am | Departure |
