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Three Dimensional Electron Microscopy

Gordon Research Conference

Opening New Frontiers with Cryo-Electron Microscopy

June 11 - 16, 2017

Chair John Briggs

Vice Chair John L. Rubinstein

Les Diablerets Conference Center

Eurotel Victoria

Les Diablerets, Vaud (fr), Switzerland

Venue and Travel Information

Conference Description

The 2017 GRC on Three-Dimensional Electron Microscopy (3DEM) continues a tradition of more than 30 years of 3DEM GRCs.

Technical breakthroughs have led to period of explosive growth in 3DEM, which has become a powerful and popular tool for studying the structures of biological objects. This has brought new challenges in sample preparation, automation, access to equipment, training, validation and dissemination.

The revolution is far from over. Ongoing developments in hardware, software, and methods are opening new frontiers – studying conformation landscapes, gaining insights into protein complex dynamics, determining the structures of smaller and more flexible proteins, and using tomography to observe the structures, conformations and spatial relationships of molecules inside cells.

At the 2017 GRC, the latest developments and the future trends in electron cryo-microscopy and cryo-electron tomography will be presented and discussed. The focus of the meeting will be technological and methodological development, as well as new and innovative ways in which 3DEM is being applied.

Conference Program

Sunday
4:00 pm - 8:00 pm	Arrival and Check-in
6:00 pm - 7:00 pm	Dinner
7:30 pm - 7:40 pm	Introductory Comments by GRC Site Staff / Welcome from the GRC Chair
7:40 pm - 9:30 pm	Keynote Session: 3DEM as a Key Tool for Structural Biology Discussion Leader: John Rubinstein (Hospital for Sick Children, Canada)
7:40 pm - 7:45 pm	Opening Remarks
7:45 pm - 8:15 pm	Bridget Carragher (New York Structural Biology Center, USA) "3DEM Entering the Mainstream: Challenges and Opportunities"
8:15 pm - 8:30 pm	Discussion
8:30 pm - 8:50 pm	Yao Cong (Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, China) "ATP Triggered Opposite Modes of Action of the ATPases in Protein Quality Control Revealed by Cryo-EM"
8:50 pm - 9:00 pm	Discussion
9:00 pm - 9:20 pm	Yifan Cheng (Howard Hughes Medical Institute / University of California, San Francisco, USA) "Ion Channel by Single Particle Cryo-EM: Influence of Disordered Nanodisc Density on Image Alignment"
9:20 pm - 9:30 pm	Discussion
Monday
7:30 am - 8:30 am	Breakfast
9:00 am - 12:30 pm	Innovative Approaches to Sample Preparation for Proteins and Cells Discussion Leader: Debbie Kelly (Virginia Tech, USA)
9:00 am - 9:15 am	Introduction by Discussion Leader
9:15 am - 9:40 am	Thomas Braun (University of Basel, Switzerland) "Microfluidic Sample Preparation for Electron Microscopy: Opportunities, Challenges and 'Visual Proteomics'"
9:40 am - 9:55 am	Discussion
9:55 am - 10:20 am	Julia Mahamid (Max Planck Institute of Biochemistry, Germany) "Molecular Views into Cellular Function by In Situ Cryo-ET"
10:20 am - 10:35 am	Discussion
10:35 am - 11:05 am	Coffee Break
11:05 am - 11:30 am	Wen Jiang (Purdue University, USA) "Affinity Cryo-EM at Near-Atomic Resolutions"
11:30 am - 11:45 am	Discussion
11:45 am - 12:10 pm	Robert Glaeser (Lawrence Berkeley National Laboratory, USA) "Avoiding the Harmful Air-Water Interface Before Vitrification"
12:10 pm - 12:25 pm	Discussion
12:25 pm - 12:30 pm	General Discussion
12:30 pm - 1:30 pm	Group Photo / Lunch
1:30 pm - 4:30 pm	Free Time
3:00 pm - 4:00 pm	Power Hour The GRC Power Hour is an optional informal gathering open to all meeting participants. It is designed to help address the challenges women face in science and support the professional growth of women in our communities by providing an open forum for discussion and mentoring. Organizer: Giovanna Scapin (Merck & Co., Inc., USA)
4:30 pm - 6:00 pm	Poster Session
6:00 pm - 8:00 pm	Learning from, Comparing to, and Going Beyond Crystallography Discussion Leader: Ardan Patwardhan (European Bioinformatics Institute, European Molecular Biology Laboratory, United Kingdom)
6:00 pm - 6:15 pm	Introduction by Discussion Leader
6:15 pm - 6:35 pm	Peijun Zhang (University of Oxford, United Kingdom) "eBIC: A New Beamline Model for the UK National CryoEM Facility"
6:35 pm - 6:50 pm	Discussion
6:50 pm - 7:10 pm	Gira Bhabha (Skirball Institute, NYU School of Medicine, USA) "Optimizing a Family of Bacterial Lipid Transporters for Cryo EM"
7:10 pm - 7:25 pm	Discussion
7:25 pm - 7:45 pm	Holger Stark (Max-Planck Institute for Biophysical Chemistry, Germany) "The COW Image Processing Suite! A Novel Concept for Cryo-EM Image Processing"
7:45 pm - 8:00 pm	Discussion
8:00 pm - 9:00 pm	Dinner
Tuesday
7:30 am - 8:30 am	Breakfast
9:00 am - 12:30 pm	Innovations in Hardware Discussion Leader: Richard Henderson (MRC Laboratory of Molecular Biology, United Kingdom)
9:00 am - 9:15 am	Introduction by Discussion Leader
9:15 am - 9:35 am	Giorgios Skiniotis (University of Michigan, USA) "Membrane Protein Structure Determination Using Volta Phase Plates"
9:35 am - 9:50 am	Discussion
9:50 am - 10:10 am	Hans-Werner Fink (University of Zurich, Switzerland) "Holography with Low-Energy Electrons: A Tool for Single Molecule Structural Biology"
10:10 am - 10:25 am	Discussion
10:25 am - 10:55 am	Coffee Break
10:55 am - 11:15 am	Paul Mooney (Gatan, Inc., USA) "Improvements in Direct Detectors for Cryo-EM"
11:15 am - 11:25 am	Discussion
11:25 am - 11:45 am	Fei Sun (Chinese Academy of Sciences, China) "eHEC, an Algorithm to Process the Raw Frames from Falcon III Camera for Landau Noise Reduction"
11:45 am - 11:55 am	Discussion
11:55 am - 12:15 pm	Jan Pieter Abrahams (University of Basel, Switzerland) "Electron Diffraction and Crystallography for Structural Biology - A Quantitive Analysis of Recent Results"
12:15 pm - 12:30 pm	Discussion
12:30 pm - 1:30 pm	Lunch
1:30 pm - 4:30 pm	Free Time
4:30 pm - 6:00 pm	Poster Session
6:00 pm - 8:00 pm	Selected Poster Presentations: Recent Breakthroughs in 3DEM Discussion Leader: Daniela Nicastro (University of Texas Southwestern Medical Center, USA)
6:00 pm - 6:10 pm	Beata Turonova (European Molecular Biology Laboratory, Germany) "NovaCTF: An Efficient Implementation for 3D-CTF Correction in Cryo-Electron Tomography"
6:10 pm - 6:15 pm	Discussion
6:15 pm - 6:25 pm	Sahradha Albert (Max Planck Institute of Biochemistry, Germany) "Guardians at the Gate: An In Situ Cryo-Electron Tomography Study of Nuclear Proteasomes"
6:25 pm - 6:30 pm	Discussion
6:30 pm - 6:40 pm	Yong Zi Tan (New York Structural Biology Center / Columbia University, USA) "Addressing Preferred Specimen Orientation in Single-Particle Cryo-EM Through Tilting"
6:40 pm - 6:45 pm	Discussion
6:45 pm - 6:55 pm	Sjors Scheres (MRC Laboratory of Molecular Biology, United Kingdom) "Cryo-EM Structures of Tau Filaments from Alzheimer's Disease Brain"
6:55 pm - 7:00 pm	Discussion
7:00 pm - 7:10 pm	Nathaniel Talledge (University of California, San Francisco, USA) "The Structural Basis for ESCRT-III Assembly"
7:10 pm - 7:15 pm	Discussion
7:15 pm - 7:25 pm	David Veesler (University of Washington, USA) "Vitrification After Multiple Rounds of Sample Application and Blotting Improves Particle Density on Cryo-Electron Microscopy Grids"
7:25 pm - 7:30 pm	Discussion
7:30 pm - 7:40 pm	Preeti Gipson (Stanford University, USA) "Morphologies of Synaptic Protein Membrane Fusion Interfaces"
7:40 pm - 7:45 pm	Discussion
7:45 pm - 7:55 pm	Benoit Zuber (University of Bern, Switzerland) "Synaptic Vesicle Exocytosis and Rapid Modifications of the Presynaptic Cytomatrix Trapped by Time-Resolved Cryo-Electron Tomography"
7:55 pm - 8:00 pm	Discussion
8:00 pm - 9:00 pm	Dinner
Wednesday
7:30 am - 8:30 am	Breakfast
9:00 am - 12:30 pm	Structures In Situ: Tomography and Beyond Discussion Leader: Giulia Zanetti (Birkbeck, University of London, United Kingdom)
9:00 am - 9:15 am	Introduction by Discussion Leader
9:15 am - 9:35 am	Martin Pilhofer (ETH Zurich, Switzerland) "Bacterial Cell-Cell Interactions Studied by Cryo-Focused Ion Beam Milling and Electron Cryo-Tomography"
9:35 am - 9:50 am	Discussion
9:50 am - 10:10 am	Michael Elbaum (Weizmann Institute of Science, Israel) "New Ways to Acquire, Process, and Interpret Cryo-Tomograms Using STEM"
10:10 am - 10:25 am	Discussion
10:25 am - 10:55 am	Coffee Break
10:55 am - 11:15 am	Martin Beck (European Molecular Biology Laboratory, Germany) "The Human Nuclear Pore Complex Imaged in Focus"
11:15 am - 11:25 am	Discussion
11:25 am - 11:45 am	Cheri Hampton (Emory University, USA) " Targeting Cryo-EM Technologies to Investigate Enveloped Virus Structure"
11:45 am - 11:55 am	Discussion
11:55 am - 12:15 pm	Fred Sigworth (Yale University, USA) "Structure of the Kv1.2 Ion Channel in Liposomes"
12:15 pm - 12:30 pm	Discussion
12:30 pm - 1:30 pm	Lunch
1:30 pm - 4:30 pm	Free Time
4:30 pm - 6:00 pm	Poster Session
6:00 pm - 8:00 pm	Hybrid and Correlative Methods Discussion Leader: Ariane Briegel (Leiden University, The Netherlands)
6:00 pm - 6:15 pm	Introduction by Discussion Leader
6:15 pm - 6:35 pm	Tom Wirtz (Luxembourg Institute of Science and Technology, Luxembourg) "High-Resolution High-Sensitivity Nano-Analytics: Correlative Approach Combining Electron Microscopy and Secondary Ion Mass Spectrometry"
6:35 pm - 6:50 pm	Discussion
6:50 pm - 7:10 pm	Rainer Kaufmann (University of Oxford, United Kingdom) "Specimen-Friendly Super-Resolution Cryo-CLEM"
7:10 pm - 7:25 pm	Discussion
7:25 pm - 7:45 pm	Stephen Carter (California Institute of Technology, USA) "Development of Focused Ion Beam Milling and Cryo-CLEM Methods to Investigate a Novel Endoplasmic Reticulum Derived Vesicle"
7:45 pm - 8:00 pm	Discussion
8:00 pm - 9:00 pm	Dinner
Thursday
7:30 am - 8:30 am	Breakfast
8:30 am - 9:00 am	Business Meeting Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair
9:00 am - 12:30 pm	Innovative Approaches to Image Processing – Going Beyond a Structure Discussion Leader: Stefan Raunser (Max Planck Institute of Molecular Physiology, Germany)
9:00 am - 9:15 am	Introduction by Discussion Leader
9:15 am - 9:35 am	Nikolaus Grigorieff (Howard Hughes Medical Institute, USA) "Needles and Haystacks: A New Approach to Template Matching"
9:35 am - 9:50 am	Discussion
9:50 am - 10:10 am	Gunnar Schroeder (Forschungszentrum Juelich, Germany) "Principal Motions of Molecular Machines from Cryo-EM Data"
10:10 am - 10:25 am	Discussion
10:25 am - 10:55 am	Coffee Break
10:55 am - 11:15 am	Amit Singer (Princeton University, USA) "Revisiting Kam's Method for 3-D Ab-Initio Modelling, Validation, and Denoising"
11:15 am - 11:25 am	Discussion
11:25 am - 11:45 am	Jose-Jesus Fernandez (Spanish National Center for Biotechnology, Spain) "Alignment of Cryotomography Tilt-Series Taking the Beam-Induced Motion into Consideration"
11:45 am - 11:55 am	Discussion
11:55 am - 12:15 pm	Steven Ludtke (Baylor College of Medicine, USA) "Deep Learning for Efficient CryoET Segmentation and Heterogeneity Analysis"
12:15 pm - 12:30 pm	Discussion
12:30 pm - 1:30 pm	Lunch
1:30 pm - 4:30 pm	Free Time
4:30 pm - 6:00 pm	Poster Session
6:00 pm - 8:00 pm	Selected Poster Presentations: New Developments in 3DEM Discussion Leader: Shee-Mei Lok (Duke-NUS Medical School, Singapore)
6:00 pm - 6:10 pm	Gabriel Lander (The Scripps Research Institute, USA) "Beyond 3 Angstroms at 200 KeV"
6:10 pm - 6:15 pm	Discussion
6:15 pm - 6:25 pm	Hongwei Wang (Tsinghua University, China) "Atomic Resolution Structure Determination in Over-Focus Condition with Volta Phase Plate by Cs-Corrected Cryo-EM"
6:25 pm - 6:30 pm	Discussion
6:30 pm - 6:40 pm	Hans Elmlund (Monash University, Australia) "Improved Ab Initio 3D Reconstruction with SIMPLE/PRIME"
6:40 pm - 6:45 pm	Discussion
6:45 pm - 6:55 pm	Andres Leschziner (University of California, San Diego, USA) "Structural Basis for Eukaryotic Transcription-Coupled DNA Repair Initiation"
6:55 pm - 7:00 pm	Discussion
7:00 pm - 7:10 pm	Arjen Jakobi (European Molecular Biology Laboratory, Germany) "Model-Guided Local Sharpening of Cryo-EM Maps"
7:10 pm - 7:15 pm	Discussion
7:15 pm - 7:25 pm	Khanh Huy Bui (McGill University, Canada) "High-Resolution Structure of the Doublet Microtubule Tubulin Lattice Reveals Novel Architecture of the Outer Junction"
7:25 pm - 7:30 pm	Discussion
7:30 pm - 7:40 pm	Ana Casañal (MRC Laboratory of Molecular Biology, United Kingdom) "A Hub for 3'-End Processing: Structural Insights into mRNA Polyadenylation"
7:40 pm - 7:45 pm	Discussion
7:45 pm - 7:55 pm	Ricardo Righetto (University of Basel, Switzerland) "Classification Reveals Asymmetric Conformations of the MloK1 Potassium Channel from 2D Crystals"
7:55 pm - 8:00 pm	Discussion
8:00 pm - 9:00 pm	Dinner
Friday
7:30 am - 8:30 am	Breakfast
9:00 am	Departure

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