Sunday
2:00 pm - 8:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Site Staff / Welcome from the GRC Chair
7:40 pm - 9:30 pm
Keynote Session: Breakthroughs in the Studies of Biological Structures
Discussion Leader: Hongwei Wang (Tsinghua University, China)
7:40 pm - 7:50 pm
Opening Remarks
7:50 pm - 8:25 pm
Robert Glaeser (Lawrence Berkeley National Laboratory, USA)
"Aiming for 'Routinely Perfect' Single-Particle Grids"
8:25 pm - 8:40 pm
Discussion
8:40 pm - 9:15 pm
Xiaoliang Sunney Xie (Peking University, China)
"3D Structures of the Human Genome"
9:15 pm - 9:30 pm
Discussion
Monday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Cutting-Edge Technologies in Specimen Preparation and Data Acquisition
Discussion Leader: Gabriel Lander (The Scripps Research Institute, USA)
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:45 am
Yong Zi Tan (Columbia University / New York Structural Biology Center, USA)
"Many Ways to Skin a Cat: Strategies Used to Solve Structures of 3 Small Asymmetric Membrane Proteins"
9:45 am - 10:00 am
Discussion
10:00 am - 10:30 am
Group Photo / Coffee Break
10:30 am - 10:55 am
Elizabeth Kellogg (Cornell University, USA)
"Cryo-EM Structure of the P Element Transposase Strand Transfer Complex"
10:55 am - 11:10 am
Discussion
11:10 am - 11:35 am
Hailin Peng (Peking University, China)
"Scalable Fabrication of High-Quality Graphene Grids for Atomic-Resolution EM"
11:35 am - 11:50 am
Discussion
11:50 am - 12:15 pm
Rebecca Thompson (University of Leeds, United Kingdom)
"Trials and Tribulations of an EM Facility Manager"
12:15 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
3:00 pm - 4:00 pm
The GRC Power Hourâ„¢
The GRC Power Hourâ„¢ is designed to address challenges women face in science and issues of diversity and inclusion. The program supports the professional growth of all members of our communities by providing an open forum for discussion and mentoring.
Organizers: Sharon Wolf (Weizmann Institute of Science, Israel) and Isabelle Rouiller (University of Melbourne, Australia)
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Strategies to Push the Resolution Limit of Structural Determination
Discussion Leader: Nieng Yan (Princeton University, USA)
7:30 pm - 7:45 pm
Introduction by Discussion Leader
7:45 pm - 8:05 pm
Edward Egelman (University of Virginia, USA)
"Cryo-EM of Helical Polymers at Near-Atomic Resolution Yields Some Surprises"
8:05 pm - 8:20 pm
Discussion
8:20 pm - 8:40 pm
Koji Yonekura (RIKEN SPring-8 Center, Japan)
"A New Cryo-EM System for Single Particle Analysis and Electron 3D Crystallography"
8:40 pm - 8:55 pm
Discussion
8:55 pm - 9:15 pm
Tao Ni (University of Oxford, United Kingdom)
"CryoEM Structure of HIV-1 Capsid in Complex with Host Factor Cyclophilin A"
9:15 pm - 9:30 pm
Discussion
Tuesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Discoveries Beyond Three Dimensional Structures
Discussion Leader: Masahide Kikkawa (The University of Tokyo, Japan)
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:45 am
Huilin Li (Van Andel Research Institute, USA)
"Cryo-EM in DNA Replication: From Replication Forks to Replication Factories"
9:45 am - 10:00 am
Discussion
10:00 am - 10:25 am
John Rubinstein (The Hospital for Sick Children, Canada)
"Scarce and Unstable Membrane Protein Complexes"
10:25 am - 10:40 am
Discussion
10:40 am - 11:10 am
Coffee Break
11:10 am - 11:35 am
Hugues Nury (CNRS, France)
"Serotonin 5-HT3 Receptors: Structures and Transitions"
11:35 am - 11:50 am
Discussion
11:50 am - 12:15 pm
Melody Campbell (University of California, San Francisco, USA)
"Visualizing Integrin Signaling Dynamics"
12:15 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
2:00 pm - 4:00 pm
Poster Session
4:00 pm - 6:00 pm
Selected Poster Presentations: Recent Breakthroughs in 3DEM
Discussion Leader: Fred Sigworth (Yale University, USA)
4:00 pm - 4:10 pm
Eric Verbeke (University of Texas at Austin, USA)
"Single Particles from Single Cells Using Shotgun Electron Microscopy"
4:10 pm - 4:15 pm
Discussion
4:15 pm - 4:25 pm
Shabih Shakeel (MRC Laboratory of Molecular Biology, United Kingdom)
"Architecture of FA Core Complex"
4:25 pm - 4:30 pm
Discussion
4:30 pm - 4:40 pm
Donghyun Park (Yale University, USA)
"Assembly and Activation States of the Dot/Icm Type IVB Secretion System"
4:40 pm - 4:45 pm
Discussion
4:45 pm - 4:55 pm
Dovile Januliene (Max Planck Institute of Biophysics, Germany)
"Conformations of a Heterodimeric ABC Transporter Under Turnover Conditions"
4:55 pm - 5:00 pm
Discussion
5:00 pm - 5:10 pm
Patrick Hoffmann (MRC Laboratory of Molecular Biology, United Kingdom)
"Contacts Between the Endoplasmic Reticulum and the Plasma Membrane"
5:10 pm - 5:15 pm
Discussion
5:15 pm - 5:25 pm
Dmitry Lyumkis (The Salk Institute for Biological Studies, USA)
"A Relationship Between Sampling and Resolution in Single-Particle Cryo-EM"
5:25 pm - 5:30 pm
Discussion
5:30 pm - 5:40 pm
Christopher Russo (MRC Laboratory of Molecular Biology, United Kingdom)
"Energy Dependence of Radiation Damage in Electron Cryomicroscopy"
5:40 pm - 5:45 pm
Discussion
5:45 pm - 5:55 pm
Paul Mooney (Gatan, Inc., USA)
"Assessing the Performance of the K3 Camera"
5:55 pm - 6:00 pm
Discussion
6:00 pm - 7:00 pm
Dinner
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Innovations of Instruments and Hardware
Discussion Leader: Fei Sun (Chinese Academy of Sciences, China)
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:45 am
Peter Peters (Maastricht Multimodal Molecular Imaging Institute (M4I), Maastricht University, The Netherlands)
"Automated Cryo-EM Sample Preparation Employing Pin-Printing and Jet Vitrification"
9:45 am - 10:00 am
Discussion
10:00 am - 10:25 am
Sandip Kaledhonkar (Columbia University, USA)
"Time Resolved Cryo-EM"
10:25 am - 10:40 am
Discussion
10:40 am - 11:10 am
Coffee Break
11:10 am - 11:35 am
Holger Stark (Max-Planck Institute for Biophysical Chemistry, Germany)
"Are Further Improvements in Electron Microscopic Hardware Possible and Useful?"
11:35 am - 11:50 am
Discussion
11:50 am - 12:15 pm
Fu-Rong Chen (City University of Hong Kong, Hong Kong SAR China)
"Atomic Resolution 3D Dynamics of Soft Materials: From Low Dose Hologram to Interaction-Free Electron Microscopy"
12:15 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:00 pm - 7:30 pm
Business Meeting
Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair
7:30 pm - 9:30 pm
Advances of Algorithms and Software
Discussion Leader: Sjors Scheres (MRC Laboratory of Molecular Biology, United Kingdom)
7:30 pm - 7:45 pm
Introduction by Discussion Leader
7:45 pm - 8:05 pm
Carsten Sachse (Forschungszentrum Jülich, Germany)
"Interpretation of Cryo-EM Maps by Atomic Models"
8:05 pm - 8:20 pm
Discussion
8:20 pm - 8:40 pm
Carlos Oscar Sorzano (Spanish National Research Council (CSIC), Spain)
"Recent Developments in Scipion and Xmipp for the Analysis of Single Particles by Electron Microscopy"
8:40 pm - 8:55 pm
Discussion
8:55 pm - 9:15 pm
Amit Singer (Princeton University, USA)
"Towards Reconstruction Without Particle Picking"
9:15 pm - 9:30 pm
Discussion
Thursday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Correlative Methods and Large-Scale Imaging
Discussion Leader: Gaia Pigino (Max Planck Institute of Molecular Cell Biology and Genetics, Germany)
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:45 am
Giulia Zanetti (Birkbeck, University of London, United Kingdom)
"Molecular Determinants of Membrane Budding Revealed by Sub-Tomogram Averaging Studies of COPII"
9:45 am - 10:00 am
Discussion
10:00 am - 10:25 am
Guoqiang Bi (University of Science and Technology of China, China)
"Synaptic Vesicle Fusion Under Cryo-ET"
10:25 am - 10:40 am
Discussion
10:40 am - 11:10 am
Coffee Break
11:10 am - 11:35 am
Danielle Grotjahn (The Scripps Research Institute, USA)
"Visualizing Mitochondrial Fission Machinery In Situ by Cryo-Electron Tomography"
11:35 am - 11:50 am
Discussion
11:50 am - 12:15 pm
Manfred Auer (Lawrence Berkeley National Laboratory, USA)
"Cellular Signatures of a 3D Matrix Grown Human Organoid Breast Cancer Progression Model System"
12:15 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Selected Poster Presentations: New Discoveries by 3DEM
Discussion Leader: Sharon Wolf (Weizmann Institute of Science, Israel)
4:00 pm - 4:10 pm
Feng Wang (University of California, San Francisco, USA)
"Graphene Oxide Based Affinity Grids for Cryo-EM Sample Preparation"
4:10 pm - 4:15 pm
Discussion
4:15 pm - 4:25 pm
Lisa Eshun-Wilson (University of California, Berkeley, USA)
"Effects of Alpha-Tubulin Acetylation on Microtubule Structure and Stability"
4:25 pm - 4:30 pm
Discussion
4:30 pm - 4:40 pm
Xiaojie Zhang (European Molecular Biology Laboratory, Germany)
"Liquid-Like Cellular Compartments In Situ : Stress Granules"
4:40 pm - 4:45 pm
Discussion
4:45 pm - 4:55 pm
Mikhail Kudryashev (Max Planck Institute for Biophysics, Germany)
"Structure and Conformational Heterogeneity of RyR1 in Native Membranes"
4:55 pm - 5:00 pm
Discussion
5:00 pm - 5:10 pm
Ningning Li (Peking University, China)
"Structure and Assembly of PVC, an Extracellular Contractile Injection System"
5:10 pm - 5:15 pm
Discussion
5:15 pm - 5:25 pm
Matthias Wolf (Okinawa Institute of Science and Technology Graduate University, Japan)
"Superhelical Reconstruction"
5:25 pm - 5:30 pm
Discussion
5:30 pm - 5:40 pm
Radostin Danev (The University of Tokyo, Japan)
"Performance Characteristics and Practical Limitations of the Volta Phase Plate"
5:40 pm - 5:45 pm
Discussion
5:45 pm - 5:55 pm
Osip Schwartz (University of California, Berkeley, USA)
"Laser Phase Plate for Phase Contrast Transmission Electron Microscopy"
5:55 pm - 6:00 pm
Discussion
6:00 pm - 7:00 pm
Dinner
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure