Sunday
2:00 pm - 8:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Site Staff / Welcome from the GRC Chair
7:40 pm - 9:30 pm
Keynote Session: Breakthroughs in the Studies of Biological Structures
7:40 pm - 7:50 pm
Opening Remarks
7:50 pm - 8:25 pm
"Aiming for 'Routinely Perfect' Single-Particle Grids"
8:25 pm - 8:40 pm
Discussion
8:40 pm - 9:15 pm
"3D Structures of the Human Genome"
9:15 pm - 9:30 pm
Discussion
Monday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Cutting-Edge Technologies in Specimen Preparation and Data Acquisition
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:45 am
"Many Ways to Skin a Cat: Strategies Used to Solve Structures of 3 Small Asymmetric Membrane Proteins"
9:45 am - 10:00 am
Discussion
10:00 am - 10:30 am
Group Photo / Coffee Break
10:30 am - 10:55 am
"Cryo-EM Structure of the P Element Transposase Strand Transfer Complex"
10:55 am - 11:10 am
Discussion
11:10 am - 11:35 am
"Scalable Fabrication of High-Quality Graphene Grids for Atomic-Resolution EM"
11:35 am - 11:50 am
Discussion
11:50 am - 12:15 pm
"Trials and Tribulations of an EM Facility Manager"
12:15 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
3:00 pm - 4:00 pm
The GRC Power Hourâ„¢
The GRC Power Hourâ„¢ is designed to address challenges women face in science and issues of diversity and inclusion. The program supports the professional growth of all members of our communities by providing an open forum for discussion and mentoring.
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Strategies to Push the Resolution Limit of Structural Determination
7:30 pm - 7:45 pm
Introduction by Discussion Leader
7:45 pm - 8:05 pm
"Cryo-EM of Helical Polymers at Near-Atomic Resolution Yields Some Surprises"
8:05 pm - 8:20 pm
Discussion
8:20 pm - 8:40 pm
"A New Cryo-EM System for Single Particle Analysis and Electron 3D Crystallography"
8:40 pm - 8:55 pm
Discussion
8:55 pm - 9:15 pm
"CryoEM Structure of HIV-1 Capsid in Complex with Host Factor Cyclophilin A"
9:15 pm - 9:30 pm
Discussion
Tuesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Discoveries Beyond Three Dimensional Structures
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:45 am
"Cryo-EM in DNA Replication: From Replication Forks to Replication Factories"
9:45 am - 10:00 am
Discussion
10:00 am - 10:25 am
"Scarce and Unstable Membrane Protein Complexes"
10:25 am - 10:40 am
Discussion
10:40 am - 11:10 am
Coffee Break
11:10 am - 11:35 am
"Serotonin 5-HT3 Receptors: Structures and Transitions"
11:35 am - 11:50 am
Discussion
11:50 am - 12:15 pm
"Visualizing Integrin Signaling Dynamics"
12:15 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
2:00 pm - 4:00 pm
Poster Session
4:00 pm - 6:00 pm
Selected Poster Presentations: Recent Breakthroughs in 3DEM
4:00 pm - 4:10 pm
"Single Particles from Single Cells Using Shotgun Electron Microscopy"
4:10 pm - 4:15 pm
Discussion
4:15 pm - 4:25 pm
"Architecture of FA Core Complex"
4:25 pm - 4:30 pm
Discussion
4:30 pm - 4:40 pm
"Assembly and Activation States of the Dot/Icm Type IVB Secretion System"
4:40 pm - 4:45 pm
Discussion
4:45 pm - 4:55 pm
"Conformations of a Heterodimeric ABC Transporter Under Turnover Conditions"
4:55 pm - 5:00 pm
Discussion
5:00 pm - 5:10 pm
"Contacts Between the Endoplasmic Reticulum and the Plasma Membrane"
5:10 pm - 5:15 pm
Discussion
5:15 pm - 5:25 pm
"A Relationship Between Sampling and Resolution in Single-Particle Cryo-EM"
5:25 pm - 5:30 pm
Discussion
5:30 pm - 5:40 pm
"Energy Dependence of Radiation Damage in Electron Cryomicroscopy"
5:40 pm - 5:45 pm
Discussion
5:45 pm - 5:55 pm
"Assessing the Performance of the K3 Camera"
5:55 pm - 6:00 pm
Discussion
6:00 pm - 7:00 pm
Dinner
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Innovations of Instruments and Hardware
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:45 am
"Automated Cryo-EM Sample Preparation Employing Pin-Printing and Jet Vitrification"
9:45 am - 10:00 am
Discussion
10:00 am - 10:25 am
10:25 am - 10:40 am
Discussion
10:40 am - 11:10 am
Coffee Break
11:10 am - 11:35 am
"Are Further Improvements in Electron Microscopic Hardware Possible and Useful?"
11:35 am - 11:50 am
Discussion
11:50 am - 12:15 pm
"Atomic Resolution 3D Dynamics of Soft Materials: From Low Dose Hologram to Interaction-Free Electron Microscopy"
12:15 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:00 pm - 7:30 pm
Business Meeting
Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair
7:30 pm - 9:30 pm
Advances of Algorithms and Software
7:30 pm - 7:45 pm
Introduction by Discussion Leader
7:45 pm - 8:05 pm
"Interpretation of Cryo-EM Maps by Atomic Models"
8:05 pm - 8:20 pm
Discussion
8:20 pm - 8:40 pm
"Recent Developments in Scipion and Xmipp for the Analysis of Single Particles by Electron Microscopy"
8:40 pm - 8:55 pm
Discussion
8:55 pm - 9:15 pm
"Towards Reconstruction Without Particle Picking"
9:15 pm - 9:30 pm
Discussion
Thursday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Correlative Methods and Large-Scale Imaging
9:00 am - 9:20 am
Introduction by Discussion Leader
9:20 am - 9:45 am
"Molecular Determinants of Membrane Budding Revealed by Sub-Tomogram Averaging Studies of COPII"
9:45 am - 10:00 am
Discussion
10:00 am - 10:25 am
"Synaptic Vesicle Fusion Under Cryo-ET"
10:25 am - 10:40 am
Discussion
10:40 am - 11:10 am
Coffee Break
11:10 am - 11:35 am
"Visualizing Mitochondrial Fission Machinery In Situ by Cryo-Electron Tomography"
11:35 am - 11:50 am
Discussion
11:50 am - 12:15 pm
"Cellular Signatures of a 3D Matrix Grown Human Organoid Breast Cancer Progression Model System"
12:15 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Selected Poster Presentations: New Discoveries by 3DEM
4:00 pm - 4:10 pm
"Graphene Oxide Based Affinity Grids for Cryo-EM Sample Preparation"
4:10 pm - 4:15 pm
Discussion
4:15 pm - 4:25 pm
"Effects of Alpha-Tubulin Acetylation on Microtubule Structure and Stability"
4:25 pm - 4:30 pm
Discussion
4:30 pm - 4:40 pm
"Liquid-Like Cellular Compartments In Situ : Stress Granules"
4:40 pm - 4:45 pm
Discussion
4:45 pm - 4:55 pm
"Structure and Conformational Heterogeneity of RyR1 in Native Membranes"
4:55 pm - 5:00 pm
Discussion
5:00 pm - 5:10 pm
"Structure and Assembly of PVC, an Extracellular Contractile Injection System"
5:10 pm - 5:15 pm
Discussion
5:15 pm - 5:25 pm
"Superhelical Reconstruction"
5:25 pm - 5:30 pm
Discussion
5:30 pm - 5:40 pm
"Performance Characteristics and Practical Limitations of the Volta Phase Plate"
5:40 pm - 5:45 pm
Discussion
5:45 pm - 5:55 pm
"Laser Phase Plate for Phase Contrast Transmission Electron Microscopy"
5:55 pm - 6:00 pm
Discussion
6:00 pm - 7:00 pm
Dinner
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure