Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Site Staff / Welcome from the GRC Chair
7:40 pm - 9:30 pm
Keynote Session: Contributions of 3DEM to Research on Coronavirus
7:40 pm - 7:45 pm
Opening Remarks
7:45 pm - 8:00 pm
Introduction by Discussion Leader
8:00 pm - 8:20 pm
"Structure-Guided Coronavirus Vaccine Design"
8:20 pm - 8:30 pm
Discussion
8:30 pm - 8:50 pm
"Effect of Natural and Engineered Variations on Structure and Biophysics of SARS-CoV-2 Spike"
8:50 pm - 9:00 pm
Discussion
9:00 pm - 9:20 pm
"In Situ Architecture and Neutralization Mechanism of Coronaviruses"
9:20 pm - 9:30 pm
Discussion
Monday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Innovations in Algorithms and Software
9:00 am - 9:15 am
Introduction by Discussion Leader
9:15 am - 9:35 am
"Free Energies from Cryo-EM Particles"
9:35 am - 9:50 am
Discussion
9:50 am - 10:10 am
"Visualizing the Assembly and Dynamics of Massive Molecular Machines"
10:10 am - 10:25 am
Discussion
10:25 am - 10:50 am
Group Photo / Coffee Break
10:50 am - 11:10 am
"Supervised Classification of Single Molecules in Cells From 2D Images"
11:10 am - 11:20 am
Discussion
11:20 am - 11:40 am
"Measuring Resolvability of Atomic Features in CryoEM Maps Using Q-Scores"
11:40 am - 11:55 am
Discussion
11:55 am - 12:15 pm
"Energy Landscapes: Why Bother?"
12:15 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
3:00 pm - 4:00 pm
The GRC Power Hourâ„¢
The GRC Power Hourâ„¢ is designed to address challenges women face in science and issues of diversity and inclusion. The program supports the professional growth of all members of our communities by providing an open forum for discussion and mentoring.
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Shattering Resolution Limits in Cryo-EM
7:30 pm - 7:45 pm
Introduction by Discussion Leader
7:45 pm - 8:05 pm
"Solving Structures Inside Cells"
8:05 pm - 8:20 pm
Discussion
8:20 pm - 8:40 pm
"Single-Particle Cryo-EM at Atomic Resolution"
8:40 pm - 8:55 pm
Discussion
8:55 pm - 9:15 pm
"Atomic Resolution Cryo-EM: Where Are the Limits?"
9:15 pm - 9:30 pm
Discussion
Tuesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Cellular and Molecular Tomography
9:00 am - 9:15 am
Introduction by Discussion Leader
9:15 am - 9:35 am
"Travels Through Virus-Infected Cells with In Situ Cryo-ET"
9:35 am - 9:50 am
Discussion
9:50 am - 10:10 am
"CryoCARE: Content-Aware Image Restoration for Cryo-Tomography: Where We Stand and How It Might Move Forward"
10:10 am - 10:25 am
Discussion
10:25 am - 10:55 am
Coffee Break
10:55 am - 11:15 am
"Blobology 2.0: Harnessing the Full Potential of In Situ Cryo-ET"
11:15 am - 11:25 am
Discussion
11:25 am - 11:45 am
"Multi-Scale Model of a Novel Contractile Injection System"
11:45 am - 11:55 am
Discussion
11:55 am - 12:15 pm
"Visualizing Virus Infection with Correlative Multi-Scale Multi-Modal Cryo-Imaging"
12:15 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Selected Poster Presentations: Breakthrough Methods and Workflows
7:30 pm - 7:40 pm
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
"How Much Can Inelastically Scattered Electrons Contribute to Electron Cryomicroscopy of Biological Specimens?"
7:55 pm - 8:00 pm
Discussion
8:00 pm - 8:10 pm
"Nucleotide State Influences the Equilibrium of the Origin Recognition Complex"
8:10 pm - 8:15 pm
Discussion
8:15 pm - 8:25 pm
"Structural and Functional Insights into a Peroxisomal Fatty-Acid Transporter"
8:25 pm - 8:30 pm
Discussion
8:30 pm - 8:40 pm
"Structural Parasitology of Malaria Parasites"
8:40 pm - 8:45 pm
Discussion
8:45 pm - 8:55 pm
"Quantitative Analysis of Influenza A Virus Using Convolutional Neural Networks and Cryo-Electron Tomography"
8:55 pm - 9:00 pm
Discussion
9:00 pm - 9:10 pm
"Dual-Axis CSTET: A Clearer Window into the Native Cell"
9:10 pm - 9:15 pm
Discussion
9:15 pm - 9:25 pm
"Benefits of Accurate Modeling of Electrostatic Potential Maps of Proteins"
9:25 pm - 9:30 pm
Discussion
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Sample Preparation and Workflows
9:00 am - 9:15 am
Introduction by Discussion Leader
9:15 am - 9:35 am
"Rapid Melting and Revitrification of Cryo Samples: Towards Microsecond Time-Resolved Cryo-EM"
9:35 am - 9:50 am
Discussion
9:50 am - 10:10 am
"The Potential of Movement-Free Cryo-EM"
10:10 am - 10:25 am
Discussion
10:25 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
"Light-Up Lamellae: Integrative Correlative CryoFIB Milling"
11:10 am - 11:25 am
Discussion
11:25 am - 11:45 am
"Spot the Difference: DNA Origami as Molecular Markers for CryoET"
11:45 am - 11:55 am
Discussion
11:55 am - 12:15 pm
"Solving Structures of Small Membrane Proteins"
12:15 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Hardware and Instrument Developments
7:30 pm - 7:45 pm
Introduction by Discussion Leader
7:45 pm - 8:05 pm
"Laser-Assisted Electron Microscopy: The Laser Phase Plate and Beyond"
8:05 pm - 8:20 pm
Discussion
8:20 pm - 8:40 pm
"Optimisation of Electron Detection, Electron Energy and Specimen Temperature"
8:40 pm - 8:55 pm
Discussion
8:55 pm - 9:15 pm
"Real-Time Defocus Modulation for Better Information Extraction in Cryo-EM"
9:15 pm - 9:30 pm
Discussion
Thursday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Business Meeting
Nominations for the Next Vice Chair; Fill in Conference Evaluation Forms; Discuss Future Site and Scheduling Preferences; Election of the Next Vice Chair
9:00 am - 12:30 pm
3DEM in Translational Research
9:00 am - 9:15 am
Introduction by Discussion Leader
9:15 am - 9:35 am
"Structure and Function of the Mammalian Mitochondrial Supercomplexes"
9:35 am - 9:50 am
Discussion
9:50 am - 10:10 am
"Streamlined Workflow for High Throughput EM to Facilitate Pharmaceutical Research"
10:10 am - 10:25 am
Discussion
10:25 am - 10:50 am
Coffee Break
10:50 am - 11:10 am
"Analysis of Continuous Conformational Dynamics of TMEM16 Proteins Reveals Mechanisms of Inhibitor Drugs"
11:10 am - 11:20 am
Discussion
11:20 am - 11:40 am
"Structural Basis of Substrate Shuttling in Type I Fatty Acid Synthases"
11:40 am - 11:55 am
Discussion
11:55 am - 12:15 pm
"Go Fishing: Capturing Native Structures and Multiple Functional States by the CryoID Approach"
12:15 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Selected Poster Presentations: Solving Challenging Structures
7:30 pm - 7:40 pm
"Strategies for DNA Readout in a Chromatinized Genome"
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
"In Situ Architecture of Bacterial Cell Envelope During Division"
7:55 pm - 8:00 pm
Discussion
8:00 pm - 8:10 pm
"Current Developments in CCP-EM: Servalcat and the CCP-EM Pipeliner"
8:10 pm - 8:15 pm
Discussion
8:15 pm - 8:25 pm
"Methanogenesis Megacomplex"
8:25 pm - 8:30 pm
Discussion
8:30 pm - 8:40 pm
"Solving Challenging Structures: XKR9"
8:40 pm - 8:45 pm
Discussion
8:45 pm - 8:55 pm
"Mechanism of Chromatin Regulation by Histone Variants"
8:55 pm - 9:00 pm
Discussion
9:00 pm - 9:10 pm
"In Situ Cryo-Electron Tomography of the ER-Mitochondria Encounter Structure in Yeast"
9:10 pm - 9:15 pm
Discussion
9:15 pm - 9:30 pm
General Discussion
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure