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Three Dimensional Electron Microscopy (GRS)

Gordon-Kenan Research Seminar

Novel Techniques and Breakthroughs in 3DEM of Biological Structures

June 8 - 9, 2019

Chairs

and

Conference Description

The Gordon Research Seminar on Three Dimensional Electron Microscopy is a unique forum for graduate students, post-docs, and other scientists with comparable levels of experience and education to present and exchange new data and cutting edge ideas.

The aim of this meeting is to provide an ample opportunity to the junior investigators to network and spark future collaborations. As the three dimensional electron microscopy is moving forward in a very fast pace, the young scientists with diverse backgrounds from X-ray crystallography to correlative microscopy attending the GRS will spearhead the cryo-EM field and shape future GRCs as well.

Related Meeting

This GRS will be held in conjunction with the "Three Dimensional Electron Microscopy" Gordon Research Seminar (GRS). Those interested in attending both meetings must submit an application for the GRC in addition to an application for the GRS. Refer to the associated GRC program page for more information.

Conference Program

Saturday
2:00 pm - 5:00 pm	Arrival and Check-in
3:30 pm - 3:45 pm	Introductory Comments by GRC Site Staff / Welcome from the GRS Chair
3:45 pm - 4:30 pm	Keynote Session: Stories from Newly Independent Cryo-EMers Discussion Leader:
3:45 pm - 3:50 pm	Introduction by Discussion Leader
3:50 pm - 4:05 pm	"Structural Basis of Lipopolysaccharide Extraction from the Bacterial Inner Membrane"
4:05 pm - 4:10 pm	Discussion
4:10 pm - 4:25 pm	"High-Resolution Cryo-EM Studies of Protein Complexes Involved in Cell Cycle Regulation"
4:25 pm - 4:30 pm	Discussion
4:30 pm - 6:00 pm	Poster Session
6:00 pm - 7:00 pm	Dinner
7:30 pm - 9:30 pm	Challenges Within EM Sample Preparation Discussion Leader:
7:30 pm - 7:40 pm	"Giant Marine Virus Sample Preparation and Data Collection for Cryo-EM"
7:40 pm - 7:45 pm	Discussion
7:45 pm - 7:55 pm	"The Battle of the Titans: Phd Student vs. Mitochondrial ATP-Dependent Proteases"
7:55 pm - 8:00 pm	Discussion
8:00 pm - 8:10 pm	"In Situ Protein Micro-Crystal Fabrication by Cryo-FIB for Electron Diffraction"
8:10 pm - 8:15 pm	Discussion
8:15 pm - 8:25 pm	"The Molecular Architecture of the Cl-Transporter SLC26A9"
8:25 pm - 8:30 pm	Discussion
8:30 pm - 8:40 pm	"In Situ Structure and Arrangement of Influenza Virus Matrix Protein M1 by Cryo Electron Tomography"
8:40 pm - 8:45 pm	Discussion
8:45 pm - 8:55 pm	"Deciphering the Structural Basis of Liquid-Like Cellular Compartments In Situ: Stress Granules as a Case Study"
8:55 pm - 9:00 pm	Discussion
9:00 pm - 9:10 pm	"Linking Architecture and Function of Contacts Between the Endoplasmic Reticulum and the Plasma Membrane"
9:10 pm - 9:15 pm	Discussion
9:15 pm - 9:25 pm	"Problems and Solutions in Cryo-Electron Tomography of Cellular Samples"
9:25 pm - 9:30 pm	Discussion
Sunday
7:30 am - 8:30 am	Breakfast
9:00 am - 11:00 am	Challenges Within EM Data Collection and Processing Discussion Leader:
9:00 am - 9:10 am	"Robustness and Feasibility of Fast-Incremental Tilt Series Acquisition"
9:10 am - 9:15 am	Discussion
9:15 am - 9:25 am	"TOPAZ: Positive-Unlabeled Convolutional Neural Network Particle Picker that Can Pick Any Size and Shape Particle"
9:25 am - 9:30 am	Discussion
9:30 am - 9:40 am	"Cryo-EM Snapshots of the Injectisome Needle Complex Reveal the Basis of Assembly"
9:40 am - 9:45 am	Discussion
9:45 am - 9:55 am	"Architecture of FA Core Complex"
9:55 am - 10:00 am	Discussion
10:00 am - 10:10 am	"Structural Investigation into the HIV RNA Nuclear Export Complex"
10:10 am - 10:15 am	Discussion
10:15 am - 10:25 am	"Single Particle Cryo-EM Reconstruction of 52 kDa Streptavidin at 3.2 Angstrom Resolution"
10:25 am - 10:30 am	Discussion
10:30 am - 10:40 am	"Snapshots of Single Particles from Single Cells Using Shotgun Electron Microscopy"
10:40 am - 10:45 am	Discussion
10:45 am - 10:55 am	"Thresholding of Cryo-EM Density Maps by False Discovery Rate Control"
10:55 am - 11:00 am	Discussion
11:00 am - 12:30 pm	Poster Session Coffee will be served in the poster area from 11:00 am - 11:30 am
12:30 pm - 1:30 pm	Lunch
1:30 pm - 2:30 pm	Mentorship Component: Career Pathways in Cryo-EM Discussion Leader:
1:30 pm - 2:30 pm	Panel Discussion Career Pathways in Cryo-EM
2:30 pm - 3:00 pm	Evaluation Period Fill in GRS Evaluation Forms
3:00 pm	Seminar Concludes

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Three Dimensional Electron Microscopy

June 15 - 20, 2025

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