Saturday
2:00 pm - 5:00 pm
Arrival and Check-in
3:30 pm - 3:45 pm
Introductory Comments by GRC Site Staff / Welcome from the GRS Chair
3:45 pm - 4:30 pm
Keynote Session: Stories from Newly Independent Cryo-EMers
Discussion Leader: Lisa Eshun-Wilson (University of California, Berkeley, USA)
3:45 pm - 3:50 pm
Introduction by Discussion Leader
3:50 pm - 4:05 pm
Yanyan Li (Westlake University, China)
"Structural Basis of Lipopolysaccharide Extraction from the Bacterial Inner Membrane"
4:05 pm - 4:10 pm
Discussion
4:10 pm - 4:25 pm
Andreas Boland (University of Geneva, Switzerland)
"High-Resolution Cryo-EM Studies of Protein Complexes Involved in Cell Cycle Regulation"
4:25 pm - 4:30 pm
Discussion
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Challenges Within EM Sample Preparation
Discussion Leader: Ann Varano (Pennsylvania State University, USA)
7:30 pm - 7:40 pm
Chuan Xiao (University of Texas at El Paso, USA)
"Giant Marine Virus Sample Preparation and Data Collection for Cryo-EM"
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
Cristina Puchades (Scripps Research, USA)
"The Battle of the Titans: Phd Student vs. Mitochondrial ATP-Dependent Proteases"
7:55 pm - 8:00 pm
Discussion
8:00 pm - 8:10 pm
Xinmei Li (Institute of Biophysics, Chinese Academy of Sciences, China)
"In Situ Protein Micro-Crystal Fabrication by Cryo-FIB for Electron Diffraction"
8:10 pm - 8:15 pm
Discussion
8:15 pm - 8:25 pm
Marta Sawicka (University of Zurich, Switzerland)
"The Molecular Architecture of the Cl-Transporter SLC26A9"
8:25 pm - 8:30 pm
Discussion
8:30 pm - 8:40 pm
Julia Peukes (MRC Laboratory of Molecular Biology, United Kingdom)
"In Situ Structure and Arrangement of Influenza Virus Matrix Protein M1 by Cryo Electron Tomography"
8:40 pm - 8:45 pm
Discussion
8:45 pm - 8:55 pm
Xiaojie Zhang (European Molecular Biology Laboratory, Germany)
"Deciphering the Structural Basis of Liquid-Like Cellular Compartments In Situ : Stress Granules as a Case Study"
8:55 pm - 9:00 pm
Discussion
9:00 pm - 9:10 pm
Patrick Hoffmann (MRC Laboratory of Molecular Biology, United Kingdom)
"Linking Architecture and Function of Contacts Between the Endoplasmic Reticulum and the Plasma Membrane"
9:10 pm - 9:15 pm
Discussion
9:15 pm - 9:25 pm
Aygul Ishemgulova (CEITEC, Masaryk University, Czech Republic)
"Problems and Solutions in Cryo-Electron Tomography of Cellular Samples"
9:25 pm - 9:30 pm
Discussion
Sunday
7:30 am - 8:30 am
Breakfast
9:00 am - 11:00 am
Challenges Within EM Data Collection and Processing
Discussion Leader: Zhaoming Su (Stanford University, USA)
9:00 am - 9:10 am
Fabian Eisenstein (ETH Zurich, Switzerland)
"Robustness and Feasibility of Fast-Incremental Tilt Series Acquisition"
9:10 am - 9:15 am
Discussion
9:15 am - 9:25 am
Alex Noble (Simons Electron Microscopy Center, New York Structural Biology Center, USA)
"TOPAZ: Positive-Unlabeled Convolutional Neural Network Particle Picker that Can Pick Any Size and Shape Particle"
9:25 am - 9:30 am
Discussion
9:30 am - 9:40 am
Jinhong Hu (University of British Columbia, Canada)
"Cryo-EM Snapshots of the Injectisome Needle Complex Reveal the Basis of Assembly"
9:40 am - 9:45 am
Discussion
9:45 am - 9:55 am
Shabih Shakeel (MRC Laboratory of Molecular Biology, United Kingdom)
"Architecture of FA Core Complex"
9:55 am - 10:00 am
Discussion
10:00 am - 10:10 am
Amber Smith (University of California, San Francisco, USA)
"Structural Investigation into the HIV RNA Nuclear Export Complex"
10:10 am - 10:15 am
Discussion
10:15 am - 10:25 am
Xiao Fan (Tsinghua University, China)
"Single Particle Cryo-EM Reconstruction of 52 kDa Streptavidin at 3.2 Angstrom Resolution"
10:25 am - 10:30 am
Discussion
10:30 am - 10:40 am
Eric Verbeke (University of Texas at Austin, USA)
"Snapshots of Single Particles from Single Cells Using Shotgun Electron Microscopy"
10:40 am - 10:45 am
Discussion
10:45 am - 10:55 am
Maximilian Beckers (European Molecular Biology Laboratory, Germany)
"Thresholding of Cryo-EM Density Maps by False Discovery Rate Control"
10:55 am - 11:00 am
Discussion
11:00 am - 12:30 pm
Poster Session
Coffee will be served in the poster area from 11:00 am - 11:30 am
12:30 pm - 1:30 pm
Lunch
1:30 pm - 2:30 pm
Mentorship Component: Career Pathways in Cryo-EM
Discussion Leader: Henry Nguyen (University of California, San Francisco, USA)
1:30 pm - 2:30 pm
Panel Discussion
Career Pathways in Cryo-EM
Yifan Cheng (Howard Hughes Medical Institute / University of California, San Francisco, USA)
Karin Kuehnel (Nature Communications, United Kingdom)
Lionel Rouge (Genentech, USA)
Rebecca Thompson (University of Leeds, United Kingdom)
Hongwei Wang (Tsinghua University, China)
Sharon Wolf (Weizmann Institute of Science, Israel)
Shaoxia Chen (MRC Laboratory of Molecular Biology, United Kingdom)
2:30 pm - 3:00 pm
Evaluation Period
Fill in GRS Evaluation Forms
3:00 pm
Seminar Concludes
