Saturday
2:00 pm - 5:00 pm
Arrival and Check-in
3:30 pm - 3:45 pm
Introductory Comments by GRC Site Staff / Welcome from the GRS Chair
3:45 pm - 4:30 pm
Keynote Session: Stories from Newly Independent Cryo-EMers
3:45 pm - 3:50 pm
Introduction by Discussion Leader
3:50 pm - 4:05 pm
"Structural Basis of Lipopolysaccharide Extraction from the Bacterial Inner Membrane"
4:05 pm - 4:10 pm
Discussion
4:10 pm - 4:25 pm
"High-Resolution Cryo-EM Studies of Protein Complexes Involved in Cell Cycle Regulation"
4:25 pm - 4:30 pm
Discussion
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Challenges Within EM Sample Preparation
7:30 pm - 7:40 pm
"Giant Marine Virus Sample Preparation and Data Collection for Cryo-EM"
7:40 pm - 7:45 pm
Discussion
7:45 pm - 7:55 pm
"The Battle of the Titans: Phd Student vs. Mitochondrial ATP-Dependent Proteases"
7:55 pm - 8:00 pm
Discussion
8:00 pm - 8:10 pm
"In Situ Protein Micro-Crystal Fabrication by Cryo-FIB for Electron Diffraction"
8:10 pm - 8:15 pm
Discussion
8:15 pm - 8:25 pm
"The Molecular Architecture of the Cl-Transporter SLC26A9"
8:25 pm - 8:30 pm
Discussion
8:30 pm - 8:40 pm
"In Situ Structure and Arrangement of Influenza Virus Matrix Protein M1 by Cryo Electron Tomography"
8:40 pm - 8:45 pm
Discussion
8:45 pm - 8:55 pm
"Deciphering the Structural Basis of Liquid-Like Cellular Compartments In Situ : Stress Granules as a Case Study"
8:55 pm - 9:00 pm
Discussion
9:00 pm - 9:10 pm
"Linking Architecture and Function of Contacts Between the Endoplasmic Reticulum and the Plasma Membrane"
9:10 pm - 9:15 pm
Discussion
9:15 pm - 9:25 pm
"Problems and Solutions in Cryo-Electron Tomography of Cellular Samples"
9:25 pm - 9:30 pm
Discussion
Sunday
7:30 am - 8:30 am
Breakfast
9:00 am - 11:00 am
Challenges Within EM Data Collection and Processing
9:00 am - 9:10 am
"Robustness and Feasibility of Fast-Incremental Tilt Series Acquisition"
9:10 am - 9:15 am
Discussion
9:15 am - 9:25 am
"TOPAZ: Positive-Unlabeled Convolutional Neural Network Particle Picker that Can Pick Any Size and Shape Particle"
9:25 am - 9:30 am
Discussion
9:30 am - 9:40 am
"Cryo-EM Snapshots of the Injectisome Needle Complex Reveal the Basis of Assembly"
9:40 am - 9:45 am
Discussion
9:45 am - 9:55 am
"Architecture of FA Core Complex"
9:55 am - 10:00 am
Discussion
10:00 am - 10:10 am
"Structural Investigation into the HIV RNA Nuclear Export Complex"
10:10 am - 10:15 am
Discussion
10:15 am - 10:25 am
"Single Particle Cryo-EM Reconstruction of 52 kDa Streptavidin at 3.2 Angstrom Resolution"
10:25 am - 10:30 am
Discussion
10:30 am - 10:40 am
"Snapshots of Single Particles from Single Cells Using Shotgun Electron Microscopy"
10:40 am - 10:45 am
Discussion
10:45 am - 10:55 am
"Thresholding of Cryo-EM Density Maps by False Discovery Rate Control"
10:55 am - 11:00 am
Discussion
11:00 am - 12:30 pm
Poster Session
Coffee will be served in the poster area from 11:00 am - 11:30 am
12:30 pm - 1:30 pm
Lunch
1:30 pm - 2:30 pm
Mentorship Component: Career Pathways in Cryo-EM
1:30 pm - 2:30 pm
Panel Discussion
Career Pathways in Cryo-EM
2:30 pm - 3:00 pm
Evaluation Period
Fill in GRS Evaluation Forms
3:00 pm
Seminar Concludes
