Saturday
1:00 pm - 5:00 pm
Arrival and Check-in
3:00 pm - 3:15 pm
Introductory Comments by GRC Site Staff / Welcome from the GRS Chair
3:15 pm - 4:00 pm
Structural Studies of Cryo-EM Telomerases
Discussion Leader: Cathy Spangler (Oregon Health & Science University, United States)
3:15 pm - 3:20 pm
Introduction by Discussion Leader
3:20 pm - 3:50 pm
Thi Hoang Duong (Kelly) Nguyen (MRC Laboratory of Molecular Biology, United Kingdom)
"Visualisation of Human Telomerase Holoenzyme by Cryo-EM"
3:50 pm - 4:00 pm
Discussion
4:00 pm - 5:30 pm
Poster Session
5:30 pm - 7:30 pm
Novel Sample and Grid Preparation Methods in Cryo-EM
Novel sample and grid preparation methods for cryo-EM
Discussion Leader: Shengjie Feng (UCSF, United States)
5:30 pm - 5:40 pm
Paul Sauer (UC Berkeley, United States)
"Using Streptavidin Affinity Grids to Visualize Difficult Biological Samples"
5:40 pm - 5:45 pm
Discussion
5:45 pm - 5:55 pm
Talya Levitz (Massachusetts Institute of Technology, United States)
"Use of the chamaleon for Cryo-EM Studies of Ribonucleotide Reductase"
5:55 pm - 6:00 pm
Discussion
6:00 pm - 6:10 pm
Emma Couves (Imperial College London, United Kingdom)
"Capturing Inhibited Assemblies of the Membrane Attack Complex"
6:10 pm - 6:15 pm
Discussion
6:15 pm - 6:25 pm
Eric Verbeke (Princeton University, United States)
"Don't Purify in Vein! Structural Insights from Red Blood Cell Lysate"
6:25 pm - 6:30 pm
Discussion
6:30 pm - 6:40 pm
Mykhailo Kopylov (New York Structural Biology Center - Simons Electron Microscopy Center, United States)
"FIB-Milling for Single Particle Tomography"
6:40 pm - 6:45 pm
Discussion
6:45 pm - 6:55 pm
Fabian Eisenstein (The University of Tokyo, Japan)
"Parallel Cryo-ET on in situ Lamellae"
6:55 pm - 7:00 pm
Discussion
7:00 pm - 7:10 pm
Bradley Guilliams (Colorado State University, United States)
"A Clonable Selenium Nanoparticle (cSeNP)"
7:10 pm - 7:15 pm
Discussion
7:15 pm - 7:30 pm
General Discussion
8:00 pm - 9:00 pm
Dinner
Sunday
7:30 am - 8:30 am
Breakfast
9:00 am - 11:00 am
Emerging Tools for Studying Conformational Variability by Cryo-EM
Emerging tools for studying conformational landscapes by cryo-EM and their biological applications
Discussion Leader: Roger Castells Graells (University of California Los Angeles, United States)
9:00 am - 9:10 am
Steve Bonilla (University of Colorado, School of Medicine, United States)
"Dissecting the Dynamic Conformational Landscape of Functional RNAs by Cryo-EM"
9:10 am - 9:15 am
Discussion
9:15 am - 9:25 am
Sepideh Valimehr (The University of Melbourne, Australia)
"Conformation and Dynamics of the AAA ATPase p97"
9:25 am - 9:30 am
Discussion
9:30 am - 9:40 am
Ottilie von Loeffelholz (CBI-IGBMC, France)
"Using Volta Phase Plate to Study Heterogenous Human Translation Initiation Complexes"
9:40 am - 9:45 am
Discussion
9:45 am - 9:55 am
Christopher JF Cameron (Yale University, United States)
"Particles of Confederation - Reliably Expanding Particle Sets by Machine-Learning Consensus"
9:55 am - 10:00 am
Discussion
10:00 am - 10:10 am
David Herreros (Spanish National Center for Biotechnology, CSIC, Spain)
"Zernikes3D: A Continuous Heterogeneity Tool for Cryo-EM Maps and Atomic Models"
10:10 am - 10:15 am
Discussion
10:15 am - 10:25 am
Mohamad Harastani (Sorbonne University - IMPMC, France)
"TomoFlow: A New Method for Analyzing Heterogeneous Subtomogram Datasets"
10:25 am - 10:30 am
Discussion
10:30 am - 10:40 am
Zunlong Ke (Max Planck Institute of Biochemistry, Germany)
"In-situ Analysis of SARS-CoV-2 Spike Proteins on Intact Virions"
10:40 am - 10:45 am
Discussion
10:45 am - 10:55 am
Eduardo Cruz-Chu (University of Wisconsin - Milwaukee, United States)
"Retrieving Biological Function from Time-Resolved Single-Particle Cryo-EM"
10:55 am - 11:00 am
Discussion
11:00 am - 12:30 pm
Poster Session
Coffee will be served in the poster area from 11:00 am - 11:30 am
12:30 pm - 1:30 pm
Lunch
1:30 pm - 2:30 pm
Mentorship Component: Scientific Publishing
Discussion Leader: Luiza Mendonca (University of Oxford, United Kingdom)
1:30 pm - 1:35 pm
Introduction by Discussion Leader
1:35 pm - 1:55 pm
Valda Vinson (Science, AAAS, United States)
"Behind the Scenes: An Editor's Perspective on Publishing"
1:55 pm - 2:00 pm
Discussion
2:00 pm - 2:30 pm
Panel Discussion
Scientific Publishing
Radostin Danev (The University of Tokyo, Japan)
Daniela Nicastro (University of Texas Southwestern Medical Center, United States)
Thi Hoang Duong (Kelly) Nguyen (MRC Laboratory of Molecular Biology, United Kingdom)
Valda Vinson (Science, AAAS, United States)
2:30 pm - 3:00 pm
Evaluation Period
Fill in GRS Evaluation Forms
3:00 pm
Seminar Concludes
