Saturday
1:00 pm - 5:00 pm
Arrival and Check-in
3:30 pm - 3:45 pm
Introductory Comments by GRC Staff / Welcome and Introduction from the Chairs
3:45 pm - 4:30 pm
Keynote Session: The Power of 3DEM in Cellular and Molecular Biology
Discussion Leader: Joshua Hutchings (University of California San Diego, United States)
3:45 pm - 3:50 pm
Introduction by Discussion Leader
3:50 pm - 4:05 pm
Elizabeth Wright (University of Wisconsin-Madison, United States)
"Developing Technologies for Correlative Cryo-Imaging Pipelines"
4:05 pm - 4:10 pm
Discussion
4:10 pm - 4:25 pm
Alexander Sobolevsky (Columbia University, United States)
"Structural Mechanism of Heat-Induced Opening of a Temperature-Sensitive TRP Channel"
4:25 pm - 4:30 pm
Discussion
4:30 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Revolutionizing Biology Using 3DEM
Discussion Leader: Dawid Zyla (La Jolla Institute for Immunology, United States)
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 7:50 pm
Iva Ganeva (University of Bern, Switzerland)
"Understanding Cidec-Mediated Neutral Lipid Transfer Between Lipid Droplets In situ and In vitro "
7:50 pm - 7:55 pm
Discussion
7:55 pm - 8:05 pm
Giedre Ratkeviciute (University of Birmingham, United Kingdom)
"Elucidating Lipid Transport Systems in Bdellovibrio Bacteriovorus"
8:05 pm - 8:10 pm
Discussion
8:10 pm - 8:20 pm
Yang Yang (MRC-LMB, United Kingdom)
"Structures of a-Synuclein Filaments from Human Brains with Lewy Pathology"
8:20 pm - 8:25 pm
Discussion
8:25 pm - 8:35 pm
Sabrina Berkamp (Forschungszentrum Jülich, Germany)
"In situ Structural Organization of an Autophagy Cargo Receptor Studied with a Correlative Light and Electron Microscopy Pipeline"
8:35 pm - 8:40 pm
Discussion
8:40 pm - 8:50 pm
Amanda Erwin (University of Michigan, United States)
"Investigating the Architecture of Tau Aggregates In situ with Cryo-CLEM and Cryo-ET"
8:50 pm - 8:55 pm
Discussion
8:55 pm - 9:05 pm
David Dai (University of Toronto, Canada)
"Structural Studies of A Dynamic Human Centrosomal Scaffold by Electron Microscopy"
9:05 pm - 9:10 pm
Discussion
9:10 pm - 9:20 pm
Margot Riggi (Department of Biochemistry, University of Utah, Salt Lake City, USA, United States)
"Integrating Structural and Dynamic Data within 3D Molecular Animations"
9:20 pm - 9:25 pm
Discussion
9:25 pm - 9:30 pm
General Discussion
Sunday
7:30 am - 8:30 am
Breakfast
9:00 am - 11:00 am
Novel Technical and Software Developments in 3DEM
Discussion Leader: Olivia Pfeil-Gardiner (Max Planck Institute of Biophysics, Germany)
9:00 am - 9:10 am
Introduction by Discussion Leader
9:10 am - 9:20 am
Behrouz Ghazi Esfahani (Florida State University, United States)
"Lipid Nanotubules Enable Optimized Specimen Preparation and Processing For Cryo-EM"
9:20 am - 9:25 am
Discussion
9:25 am - 9:35 am
Makaía Papasergi-Scott (Stanford University, United States)
"Time-Resolved Cryo-EM of G Protein Activation by a GPCR"
9:35 am - 9:40 am
Discussion
9:40 am - 9:50 am
Jingwei Xu (Institute of molecular biology and biophysics, ETH Zürich, Switzerland)
"FilamentID: An Integrated CryoEM Approach to Identify Metabolic Filament Assemblies"
9:50 am - 9:55 am
Discussion
9:55 am - 10:05 am
Herman Fung (EMBL Heidelberg, Germany)
"Genetically Encoded Multimeric (GEM) Tags for Intracellular Protein Localization in Cellular Cryo-ET"
10:05 am - 10:10 am
Discussion
10:10 am - 10:20 am
Petar Petrov (University of California, Berkeley, United States)
"Cryo-Electron Microscopy with a Laser Phase Plate"
10:20 am - 10:25 am
Discussion
10:25 am - 10:35 am
Bjoern Forsberg (Karolinska Institute, Sweden)
"OccuPy: Heterogeneity- and Occupancy-Aware Cryo-EM Analysis Based on Spatial Filtering"
10:35 am - 10:40 am
Discussion
10:40 am - 10:50 am
Rémi Vuillemot (CNRS - IMPMC, France)
"Hybrid Methods Combining MD Simulations & Cryo-EM"
10:50 am - 10:55 am
Discussion
10:55 am - 11:00 am
General Discussion
11:00 am - 12:30 pm
Poster Session
Coffee will be served in the poster area from 11:00 am - 11:30 am
12:30 pm - 1:30 pm
Lunch
1:30 pm - 2:30 pm
Mentorship Component: Career Paths in 3DEM
Discussion Leaders: Judy Huang (University of Massachusetts Chan Medical School, United States) and Sven Klumpe (Max-Planck-Institute for Biochemistry, Germany)
1:30 pm - 1:40 pm
Introduction by Discussion Leader
1:40 pm - 2:30 pm
Panel Discussion
Career Panel
Chris Arthur (Altos Labs, United States)
Alexander Myasnikov (DCI/EPFL, Switzerland)
Bridget Carragher (Chan Zuckerberg Imaging Institute, United States)
Sharon Wolf (Weizmann Institute of Science, Israel)
Juergen Plitzko (Max Planck Institute of Biochemistry, Germany)
Radostin Danev (The University of Tokyo, Japan)
2:30 pm - 3:00 pm
Evaluation Period
Complete the GRS Evaluation Forms; Election of Future Chair(s)
3:00 pm
Seminar Concludes
