Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Staff / Welcome and Introduction from the Chairs
7:40 pm - 9:30 pm
Keynote Session: Key vEM Technologies
7:40 pm - 8:10 pm
"Visualizing Biology Across Scales using Enhanced FIB-SEM"
8:10 pm - 8:15 pm
Discussion
8:15 pm - 8:45 pm
"Volume Electron Microscopy on a Platform : From Large Volume to Cryo-NanoSIMs"
8:45 pm - 8:50 pm
Discussion
8:50 pm - 9:20 pm
"Cerebral Cortex Connectomics"
9:20 pm - 9:25 pm
Discussion
9:25 pm - 9:30 pm
General Discussion
Monday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Group Photo
9:00 am - 12:30 pm
9:00 am - 9:05 am
Introduction by Discussion Leader
9:05 am - 9:35 am
"Elucidation of Candidate Functional Nano Architectures in Metastatic Cancer Using vEM"
9:35 am - 9:45 am
Discussion
9:45 am - 10:00 am
"Volume EM Imaging at the Service of Infectious Diseases"
10:00 am - 10:05 am
Discussion
10:05 am - 10:20 am
"Applying Deep Learning Segmentation and vEM to Characterise the Male Reproductive System in a Murine Ciliopathy Model"
10:20 am - 10:25 am
Discussion
10:25 am - 11:00 am
Coffee Break
11:00 am - 11:30 am
"Volume Electron Microscopy to Study the in situ Structure of the Beta Cell Primary Cilium"
11:30 am - 11:40 am
Discussion
11:40 am - 11:55 am
"FIB-SEM 3D CLEM Analysis of Dystrophic Dopaminergic Axons in the Striatum of Mice Harboring the R258Q Parkinson Mutation in Synaptojanin 1"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:15 pm
"Elucidating the Function of MIM in Pre-Fusion Domains on Large Secretory Vesicles using 3D-Correlative FIB-SEM"
12:15 pm - 12:20 pm
Discussion
12:20 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
3:00 pm - 4:00 pm
The GRC Power Hour™
The GRC Power Hour™ is designed to address diversity and inclusion in the scientific workplace by providing a safe environment for informal and meaningful conversations amongst colleagues of all career stages. The program supports the professional growth of all members of our communities, including ethnicity, race and/or gender identity by providing an open forum for discussion and mentoring.
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Tackling Sample Preparation Challenges for vEM
7:30 pm - 7:35 pm
Introduction by Discussion Leader
7:35 pm - 8:05 pm
"How to Magnetically Collect Tissue Sections and What To Do With Them"
8:05 pm - 8:10 pm
Discussion
8:10 pm - 8:25 pm
"Possible Alternative Contrasting Agents for the SBF-SEM of Biological Samples"
8:25 pm - 8:30 pm
Discussion
8:30 pm - 9:00 pm
"Automated Sample Preparation for Pre-Clinical and Niche Applications of Volume Electron Microscopy"
9:00 pm - 9:05 pm
Discussion
9:05 pm - 9:20 pm
"Resin Comparison for SBF-SEM"
9:20 pm - 9:25 pm
Discussion
9:25 pm - 9:30 pm
General Discussion
Tuesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Molecular Labelling Strategies for vEM
9:00 am - 9:05 am
Introduction by Discussion Leader
9:05 am - 9:35 am
9:35 am - 9:45 am
Discussion
9:45 am - 10:15 am
"Correlative Light and Electron Microscopy for Mapping Cell Type-Specific Connectivity"
10:15 am - 10:25 am
Discussion
10:25 am - 11:00 am
Coffee Break
11:00 am - 11:30 am
"Enhancing the Chemical Stability of Fluorescent Proteins for Advanced Microscopy"
11:30 am - 11:40 am
Discussion
11:40 am - 11:55 am
"Multiplexed Volumetric CLEM Enabled by Antibody Derivatives Provides New Insights into the Cytology of the Mouse Cerebellar Cortex"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:15 pm
"Modernization of Golgi Staining Techniques for High-Resolution, 3-Dimensional Imaging of Individual Neurons"
12:15 pm - 12:20 pm
Discussion
12:20 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
High-Speed, High-Volume vEM Imaging
7:30 pm - 8:00 pm
"Increasing Acquisition Speed and Volume in FIB/SEM Tomography"
8:00 pm - 8:10 pm
Discussion
8:10 pm - 8:40 pm
"Large-Scale Connectomics with Multibeam Scanning Electron Microscopy"
8:40 pm - 8:50 pm
Discussion
8:50 pm - 9:20 pm
"Faster SEM for Volume Imaging"
9:20 pm - 9:30 pm
Discussion
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Tools for Acquiring, Processing and Analysing vEM Data
9:00 am - 9:05 am
Introduction by Discussion Leader
9:05 am - 9:35 am
"Toward Accessible, Scalable, and Interoperable Image Registration with BigWarp"
9:35 am - 9:45 am
Discussion
9:45 am - 10:00 am
"Mitochondrial Ultrastructure and its Regulation Revealed by Deep Learning-Based Analysis"
10:00 am - 10:05 am
Discussion
10:05 am - 10:20 am
"Software Tools for Assessment, Alignment, and Artefact Removal in Correlative Cryogenic Serial pFIB-SEM Imaging"
10:20 am - 10:25 am
Discussion
10:25 am - 11:00 am
Coffee Break
11:00 am - 11:30 am
"Unbiased Exploration of Cells, Tissues and Organs in VolumeEM"
11:30 am - 11:40 am
Discussion
11:40 am - 11:55 am
"Enabling VolumeEM: Building a Global Community and Resources"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:15 pm
"Democratizing Deep Learning for Image Segmentation with DeepMIB"
12:15 pm - 12:20 pm
Discussion
12:20 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Visualising and Sharing Big vEM Data
7:30 pm - 8:00 pm
"Public Archiving of vEM Data"
8:00 pm - 8:10 pm
Discussion
8:10 pm - 8:25 pm
"Aligning a 150 Teravoxel FIB-SEM Dataset of an Entire Fly Brain"
8:25 pm - 8:30 pm
Discussion
8:30 pm - 9:00 pm
"OME-Zarr: A Cloud-Optimized Bioimaging File Format with International Community Support"
9:00 pm - 9:10 pm
Discussion
9:10 pm - 9:25 pm
"Instance Segmentation of Organelles in 2D and 3D Cellular EM Images using Empanada"
9:25 pm - 9:30 pm
Discussion
Thursday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Business Meeting
Nominations for the Next Vice Chair(s); Complete the GRC Evaluation Forms; Discuss Future Dates and Venue; Election of the Next Vice Chair(s)
9:00 am - 12:30 pm
Volume Correlative Microscopy
9:00 am - 9:05 am
Introduction by Discussion Leader
9:05 am - 9:35 am
"Three Reasons to Correlate in VolumeEM"
9:35 am - 9:45 am
Discussion
9:45 am - 10:00 am
"Towards Achieving Quantitative 3D Super-Resolution Correlative Light-Electron Microscopy"
10:00 am - 10:05 am
Discussion
10:05 am - 10:20 am
"Unraveling Eyespot Development in Platynereis Dumerilii"
10:20 am - 10:25 am
Discussion
10:25 am - 11:00 am
Coffee Break
11:00 am - 11:30 am
"Targeted Volume EM using X-Ray Imaging and a Motorised Ultramicrotome"
11:30 am - 11:40 am
Discussion
11:40 am - 11:55 am
"Subcellular Structure-Function Insights in the MOR174/9 Glomerular Column Combining in Vivo 2-Photon Microscopy and Synchrotron X-Ray Holographic Nanotomography"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:15 pm
"Targeted Volume Correlative Light and Electron Microscopy of an Environmental Marine Microorganism"
12:15 pm - 12:20 pm
Discussion
12:20 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Latest Developments in the vEM Field
7:30 pm - 7:35 pm
Introduction by Discussion Leader
7:35 pm - 8:00 pm
"Array Tomography - A Flexible Volume EM Technique"
8:00 pm - 8:10 pm
Discussion
8:10 pm - 8:40 pm
"X-Ray Multiscale 3D Neuroimaging to Quantify Cellular Aging and Neurodegeneration"
8:40 pm - 8:45 pm
Discussion
8:45 pm - 9:15 pm
9:15 pm - 9:20 pm
Discussion
9:20 pm - 9:30 pm
Closing Remarks
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure