Sunday
2:00 pm - 9:00 pm
Arrival and Check-in
6:00 pm - 7:00 pm
Dinner
7:30 pm - 7:40 pm
Introductory Comments by GRC Site Staff / Welcome from the GRC Chair
7:40 pm - 9:30 pm
New Structural Techniques for the Study of Tetrapyrroles
This session is devoted to the application of new structural approaches (such as serial femtosecond X-ray crystallography) to the study of tetrpyrrole systems.
Discussion Leader: Syun-Ru Yeh (Albert Einstein College of Medicine, United States)
7:40 pm - 7:45 pm
Opening Remarks
7:45 pm - 8:10 pm
Brian Crane (Cornell University, United States)
"Bacterial Heme Proteins Involved in Sensing and Regulation"
8:10 pm - 8:20 pm
Discussion
8:20 pm - 8:45 pm
Allen Orville (Diamond Light Source, United Kingdom)
"Time-Resolved Serial Crystallography: Methods and Applications at Synchrotrons and XFELs"
8:45 pm - 8:55 pm
Discussion
8:55 pm - 9:20 pm
Hitomi Sawai (Nagasaki University (RIKEN SPring-8), Japan)
"Heme Controls the Structural Rearrangement of its Sensor Protein Mediating the Survival of Hemolytic Bacterial"
9:20 pm - 9:30 pm
Discussion
Monday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Trafficking, Signalling and Regulation by Heme
This session will focus on how tetrapyrroles, principally heme, are trafficked, can signal and play regulatory roles in eukaryotic cells.
Discussion Leader: Elizabeth Boon (Stony Brook University, United States)
9:00 am - 9:25 am
Emily Weinert (Pennsylvania State University, United States)
"Globin Coupled Sensors in Bacterial O2 and NO Signaling"
9:25 am - 9:35 am
Discussion
9:35 am - 10:00 am
Hiroaki Kitagishi (Doshisha University, Japan)
"Selective Depletion of Endogenous CO In Vitro and In Vivo by Hemoprotein Biomimetic Model Complexes"
10:00 am - 10:10 am
Discussion
10:10 am - 10:35 am
Coffee Break
10:35 am - 11:00 am
Dennis Stuehr (Cleveland Clinic, United States)
"GAPDH-Dependent Heme Trafficking and its Regulation"
11:00 am - 11:10 am
Discussion
11:10 am - 11:35 am
Andrew Hudson (University of Leicester, United Kingdom)
"Fluorescence Lifetime Imaging Microscopy Reveals the Mechanisms Controlling Heme Supply and Deman"
11:35 am - 11:45 am
Discussion
11:45 am - 11:55 am
Rebecca Donegan (Barnard College, United States)
"Heme and Hemoglobin Alter Biofilm Formation by Mycobacterium smegmatis "
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:10 pm
Xiaojing Yuan (University of Maryland, College Park, United States)
"Functional Adaption of Human HRG1 as an Intestinal Heme Transporter"
12:10 pm - 12:15 pm
Discussion
12:15 pm - 12:30 pm
Poster Previews
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
3:00 pm - 4:00 pm
The GRC Power Hour™
The GRC Power Hour™ is designed to address diversity and inclusion in the scientific workplace by providing a safe environment for informal and meaningful conversations amongst colleagues of all career stages. The program supports the professional growth of all members of our communities, including ethnicity, race and/or gender identity by providing an open forum for discussion and mentoring.
Organizers: Celia Goulding (University of California, Irvine, United States)
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
The Biogenesis and Functions of Bilins
Bilins are linear tetrapyrroles derived from the oxygenolytic turnover of heme found in most kingdoms of life. This session will focus on their biogenesis and diverse functions from light sensing, signaling to ROS mitigation.
Discussion Leader: J. Clark Lagarias (University of California, Davis, United States)
7:30 pm - 7:55 pm
Nicole Frankenberg-Dinkel (TU Kaiserslautern, Germany)
"Phycocyanobilin Biosynthesis in Eukaryotic Algae"
7:55 pm - 8:05 pm
Discussion
8:05 pm - 8:30 pm
Tammi Richardson (University of South Carolina, United States)
"Phycobiliproteins of Cryptophyte Microalgae"
8:30 pm - 8:40 pm
Discussion
8:40 pm - 9:05 pm
William Lanzilotta (University of Georgia, United States)
"Anaerobic Heme Degradation in Enteric Pathogens"
9:05 pm - 9:15 pm
Discussion
9:15 pm - 9:25 pm
Youzhong Guo (Virginia Commonwealth University, United States)
"TSPO Proteins Convert Protoporphyrin IX to Bilindigin, a Newly Discovered Tetrapyrrole"
9:25 pm - 9:30 pm
Discussion
Tuesday
7:30 am - 8:30 am
Breakfast
8:30 am - 9:00 am
Group Photo
9:00 am - 12:30 pm
Tetrapyrroles in Human Health and Disease
The session will explore the structure and reactivity of different tetrapyrrole systems, with an emphasis on mechanism.
Discussion Leader: Peter Meissner (University of Cape Town, South Africa)
9:00 am - 9:25 am
Makiko Yasuda (Icahn School of Medicine at Mount Sinai, United States)
"Investigation of Hepatocyte Heme Homeostasis In ALAS-Deficient Mice: The Dogma Revisited"
9:25 am - 9:35 am
Discussion
9:35 am - 10:00 am
Bruce Wang (University of California San Francisco, United States)
"Aminolevulinic Acid Synthase 2 (ALAS2) Inhibition to Treat Erythroporphyrias"
10:00 am - 10:10 am
Discussion
10:10 am - 10:35 am
Coffee Break
10:35 am - 11:00 am
Levi Wood (Georgia Institute of Technology, United States)
"Heme and Hemoglobin Down-Regulate Neuroinflammation and Glial Clearance of Amyloid Beta"
11:00 am - 11:10 am
Discussion
11:10 am - 11:35 am
Diane Ward (University of Utah School of Medicine, United States)
"The Role of Abcb10 in Mitochondrial Homeostasis"
11:35 am - 11:45 am
Discussion
11:45 am - 11:55 am
Matthew Dent (University of Pittsburgh, United States)
"From Microbial Sensor to Therapeutic Scavenger: Development of a Novel Treatment for Carbon Monoxide Poisoning Inspired by a Hemoprotein Biosensor"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:10 pm
Siva Sankari Iyer Mani Sankaran (Massachusetts Institute of Technology, United States)
"Heme-Sequestering Plant Peptide Promotes Iron Uptake in Symbiotic Bacteria-Significance and Potential Clinical Applications"
12:10 pm - 12:15 pm
Discussion
12:15 pm - 12:25 pm
Rick Homan (The Scripps Research Institute, United States)
"A Chemical Proteomic Map of Heme-Protein Interactions"
12:25 pm - 12:30 pm
Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
The Biology of Tetrapyrroles: Catalysis, Mechanism and Regulation
A session on the newest ideas of the role of heme proteins and heme metabolites in sensing and regulation.
Discussion Leader: Aimin Liu (University of Texas at San Antonio, United States)
7:30 pm - 7:55 pm
Gayan Wijeratne (The University of Alabama at Birmingham, United States)
"Modeling Mechanistic Subtleties of Nitric Oxide Synthase: A Missing Link in Understanding Human Pathogenesis"
7:55 pm - 8:05 pm
Discussion
8:05 pm - 8:30 pm
Peter Cherepanov (The Francis Crick Institute, United Kingdom)
"The Interaction of SARS-CoV-2 Spike Glycoprotein with Tetrapyrroles"
8:30 pm - 8:40 pm
Discussion
8:40 pm - 8:50 pm
Madeleine Roberts (University of Wisconsin-Madison, United States)
"Developing a Structural Model for the DNA Complex of Aerobic CO-Sensing Transcription Factor, PxRcoM-1"
8:50 pm - 8:55 pm
Discussion
8:55 pm - 9:20 pm
Matthew Liptak (University of Vermont, United States)
"The Product Distributions for Non-canonical Heme Oxygenases Depend Upon Enzyme-induced Heme Substrate Ruffling"
9:20 pm - 9:30 pm
Discussion
Wednesday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
B12: Biosynthesis, Transport, Pharmacology and Mechanistic Enzymology
This session will cover the recent discoveries of how B12 is assembled and then its involvement in important biomedical and biochemical processes. This includes its collaboration with radical SAM in a wide class of enzymatic reactions, its ability to transport RNA into cells in some cases by RNA-dependent processes, and its pharmacology.
Discussion Leader: Stephen Ragsdale (University of Michigan Medical School, United States)
9:00 am - 9:25 am
Dorota Gryko (Institute of Organic Chemistry, Polish Academy of Sciences, Poland)
"Vitamin B12 Derivatives and Conjugates for Biomedical Applications"
9:25 am - 9:35 am
Discussion
9:35 am - 10:00 am
Alison Smith (University of Cambridge, United Kingdom)
"B12 Metabolism and the Epigenome in Chlamydomonas "
10:00 am - 10:10 am
Discussion
10:10 am - 10:35 am
Coffee Break
10:35 am - 11:00 am
Ritimukta Sarangi (Stanford Synchrotron Radiation Lightsource, United States)
"X-ray Spectroscopy and Theoretical Investigations of the B12 Cofactor"
11:00 am - 11:10 am
Discussion
11:10 am - 11:35 am
Tessa Young (Durham University, United Kingdom)
"Acquisition of Cobalt for Vitamin B12 "
11:35 am - 11:45 am
Discussion
11:45 am - 11:55 am
Markus Ruetz (University of Michigan, United States)
"Inhibition of B12 Dependent Enzymes and Chaperons by Vitamin B12-Antimetabolites"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:10 pm
Yamini Mathur (Indian Institute of Science Education and Research, Pune (IISER Pune), India)
"Eukaryotic Homologs of CobT Activate Lower Ligands of Vitamin B12 and other Cobamides"
12:10 pm - 12:15 pm
Discussion
12:15 pm - 12:30 pm
Poster Previews
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:00 pm - 7:30 pm
Business Meeting
Nominations for the Next Vice Chair(s); Complete the GRC Evaluation Forms; Discuss Future Dates and Venue; Election of the Next Vice Chair(s)
7:30 pm - 9:30 pm
Regulation and Biosynthesis of Chlorophyll Pigments
This session is aimed at highlighting new details about how the primary chlorophyll pigments of photosynthesis are synthesized and understanding the regulation of photosynthetic gene expression.
Discussion Leader: Jennifer Bridwell-Rabb (University of Michigan, United States)
7:30 pm - 7:55 pm
Min Chen (University of Sydney, Australia)
"Regulation and Biosynthesis of Red-Shifted Chlorophylls: Current Status"
7:55 pm - 8:05 pm
Discussion
8:05 pm - 8:30 pm
Jean Alric (CNRS / CEA Cadarache, France)
"An Interaction Between Photosynthetic Electron Transport, Chlororespiration and Chlorophyll Biosynthesis in Chlamydomonas "
8:30 pm - 8:40 pm
Discussion
8:40 pm - 9:05 pm
Neil Hunter (University of Sheffield, United Kingdom)
"The Cyclase Step in Chlorophyll Biosynthesis"
9:05 pm - 9:15 pm
Discussion
9:15 pm - 9:30 pm
General Discussion
Thursday
7:30 am - 8:30 am
Breakfast
9:00 am - 12:30 pm
Microbial Tetrapyrrole Metabolism
This session will focus on microbial mechanisms of tetrapyrrole synthesis, uptake, utilisation, and degradation.
Discussion Leader: Mark Shepherd (University of Kent, United Kingdom)
9:00 am - 9:25 am
Celia Goulding (University of California, Irvine, United States)
"New Insights into Heme Degradation by IsdG-Type Proteins"
9:25 am - 9:35 am
Discussion
9:35 am - 10:00 am
Stefan Hofbauer (University of Natural Resources and Life Sciences, Vienna, Austria)
"Biochemistry of Enzymes Involved in the Coproporphyrin-Dependent Heme Biosynthesis Pathway of Gram-Positive Bacteria"
10:00 am - 10:10 am
Discussion
10:10 am - 10:35 am
Coffee Break
10:35 am - 11:00 am
Juliette Lecomte (Johns Hopkins University, United States)
"Taming the Heme: The Chemistry of Microbial Hemoglobins"
11:00 am - 11:10 am
Discussion
11:10 am - 11:35 am
Schara Safarian (Max-Planck-Institute of Biophysics, Germany)
"Structural and Functional Basis of Bacterial Heme Export Facilitated by CydDC"
11:35 am - 11:45 am
Discussion
11:45 am - 11:55 am
Heike Meyer (AG Layer, Pharmaceutical Biology, Albert-Ludwigs Universität Freiburg, Germany)
"Functional Characterization of the Radical SAM Enzyme NirJ Involved in Heme d1 Biosynthesis"
11:55 am - 12:00 pm
Discussion
12:00 pm - 12:10 pm
Jack Salisbury (University of Sheffield, United Kingdom)
"PufQ, a Ferrochelatase Binding Protein that Regulates Bacteriochlorophyll Biosynthesis"
12:10 pm - 12:15 pm
Discussion
12:15 pm - 12:30 pm
General Discussion
12:30 pm - 1:30 pm
Lunch
1:30 pm - 4:00 pm
Free Time
4:00 pm - 6:00 pm
Poster Session
6:00 pm - 7:00 pm
Dinner
7:30 pm - 9:30 pm
Keynote Session: Tetrapyrrole Regulation and Homeostasis
Discussion Leader: Samuel Beale (Brown University, United States)
7:30 pm - 7:40 pm
Introduction by Discussion Leader
7:40 pm - 8:20 pm
Angela Wilks (University of Maryland, United States)
"Biliverdin: A Green Light for Heme Utilization and Virulence in Pseudomonas aeruginosa "
8:20 pm - 8:35 pm
Discussion
8:35 pm - 9:15 pm
Mark O'Brian (University at Buffalo, United States)
"Heme: A Pigment on the Bacterial Palette and the Vegan Palate"
9:15 pm - 9:30 pm
Discussion
Friday
7:30 am - 8:30 am
Breakfast
9:00 am
Departure
